A Characterization of Substrates and Factors Involved in Yeast Nonsense-mediated MRNA Decay PDF Download
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Author: Jonathan Philip Belk
Publisher:
ISBN:
Category :
Languages : en
Pages : 370
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Author: Jonathan Philip Belk
Publisher:
ISBN:
Category :
Languages : en
Pages : 370
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Author: Ying Cui
Publisher:
ISBN:
Category :
Languages : en
Pages : 422
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Author:
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Category :
Languages : en
Pages :
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Author: Lynne E. Maquat
Publisher: CRC Press
ISBN: 1498713394
Category : Science
Languages : en
Pages : 277
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Book Description
Nonsense-Mediated mRNA Decay is the first book devoted to nonsense-mediated mRNA decay (NMD). The rationale for such a book is the enormous information that studies of NMD have provided on the intricacies of post-transcriptional gene expression. The first five sections of the book are divided according to organism and begin with chapters on S. cere
Author: Alan Baer Maderazo
Publisher:
ISBN:
Category :
Languages : en
Pages : 354
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Author:
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Category :
Languages : en
Pages : 222
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Author: Lucas D. Serdar
Publisher:
ISBN:
Category : Gene expression
Languages : en
Pages : 147
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The fidelity of gene expression depends on quality control pathways that act to detect and resolve errors in the transmission of genetic information. Premature termination of translation at nonsense codons within mRNA open reading frames leads to the production of C-terminally truncated polypeptides with potentially deleterious functions in the cell. The nonsense-mediated mRNA decay (NMD) pathway is a conserved quality control pathway in eukaryotes that limits accumulation of these aberrant protein products by recognizing the nonsense-containing mRNA and targeting it to accelerated degradation. Recognition and rapid destabilization of NMD substrates requires a conserved machinery consisting of the proteins UPF1, UPF2, and UPF3. Recent transcriptome-wide studies have shown that the RNA helicase UPF1 associates with both nonsense-containing and normal mRNAs, suggesting that the mechanistic distinction between normal and aberrant mRNAs occurs after UPF1 associates with the transcript. To investigate the requirements for NMD at steps downstream of UPF1 association with mRNA, we applied tethered function analysis in yeast cells and found that destabilization of mRNAs by tethered UPF1 requires ATP hydrolysis by UPF1, and is enhanced by premature termination. Surprisingly, the activity of tethered UPF1 was robust upon inhibition of translation, and in cells lacking UPF2 and UPF3. Next, we provide evidence that ATP hydrolysis by UPF1 is required for efficient translation termination and ribosome release at a premature termination codon. Ribosome stalling at or near premature termination codons in UPF1 ATPase mutants impedes the progress of the exonuclease XRN1, leading to the accumulation of 3’ RNA decay fragments. We show also that the ability of UPF1 to impinge upon premature termination requires NMD co-factors UPF2 and UPF3. Unexpectedly, ribosome stalling in cells expressing ATPase-deficient UPF1 occurs at a position downstream of the premature stop codon. The position of stalling is dependent on the mRNA sequence downstream of the stop codon, and transit of ribosomes to these positions did not require ongoing polypeptide synthesis. Our results reveal that ATP hydrolysis by UPF1 modulates a functional interaction between the NMD machinery and terminating ribosomes necessary for targeting substrates to accelerated degradation.
Author: Lynne E. Maquat
Publisher: Academic Press
ISBN: 9780123745842
Category : Science
Languages : en
Pages : 464
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Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. * Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents
Author:
Publisher: Academic Press
ISBN: 0128024887
Category : Science
Languages : en
Pages : 412
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Book Description
Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods in visualizing RNA dynamics in the cell - Contains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe
Author: Michelle Fleur Page
Publisher:
ISBN:
Category :
Languages : en
Pages : 428
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