Volatile Biomarkers from Cultured Human Immune Cells, Cultured Human Acute Lymphoblastic Leukemia Cells and the Exhaled Breath of Patients with Acute Myelolgenous Leukemia PDF Download
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Author: Brandon James Umber
Publisher:
ISBN: 9781267171078
Category :
Languages : en
Pages : 168
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Book Description
Presented here are the volatile organic compounds (VOCs) that were observed to be produced or consumed by healthy and malignant human immune cells as well as those observed in the exhaled breath of patients with acute myelogenous leukemia (AML). Two lineages of white cells were investigated: the lymphoid and the myeloid line of cellular differentiation. The lymphoid lineage was investigated using cultured human peripheral blood mononuclear cells (PBMCs) from healthy donors and cryogenically preserved leukemic cells collected from patients with acute lymphocytic leukemia (ALL). Likewise, the myeloid lineage was investigated using mature neutrophils from healthy donors, the HL60 cell line (malignant immature neutrophils), and the whole air collection of the exhaled breath of patients with AML. Cell cultures were grown in hermetically sealable glass culture flasks and the headspace above the culture was collected for analysis. The exhaled breath of patients with AML and the exhalant of healthy controls were collected in evacuated stainless steel canisters. Analysis of the samples was performed using gas chromatography. Cultured PBMCs were shown to reduce the concentration of acetaldehyde in the headspace relative to controls. Acetaldehyde in the headspace was 1.9±1 ppbv less than what was present in the media. Likewise hexanaldehyde was lower by 0.10±0.05 ppbv. Acute lymphocytic leukemia cells produced greater amounts of acetaldehyde and dimethyl sulfide relative to controls: 14±5 ppbv and 200±50 ppbv above controls. Healthy nuetrophils in culture produced a concentration of acetaldehyde of 4.5±5.4 ppbv above control and had a level of hexanaldehyde 80±60 pptv below controls. The HL60 cells produced 11±3 ppbv of acetaldehyde and 200±70 pptv of hexanladehyde. The exhaled breath of patients with AML contained levels of five VOCs that were significantly different from those found in the healthy controls: acetaldehyde, butane, carbon disulfide, carbonyl sulfide, and heptane. Carbon disulfide and butane were present in levels above the healthy controls and the ambient room concentrations: 15±17 pptv and 700±1,300 pptv. With the goal of diagnostic efficacy in mind it is hoped that the nascent VOC profile of leukemia discussed in this work may provide a foundation for future studies.
Author: Brandon James Umber
Publisher:
ISBN: 9781267171078
Category :
Languages : en
Pages : 168
Get Book Here
Book Description
Presented here are the volatile organic compounds (VOCs) that were observed to be produced or consumed by healthy and malignant human immune cells as well as those observed in the exhaled breath of patients with acute myelogenous leukemia (AML). Two lineages of white cells were investigated: the lymphoid and the myeloid line of cellular differentiation. The lymphoid lineage was investigated using cultured human peripheral blood mononuclear cells (PBMCs) from healthy donors and cryogenically preserved leukemic cells collected from patients with acute lymphocytic leukemia (ALL). Likewise, the myeloid lineage was investigated using mature neutrophils from healthy donors, the HL60 cell line (malignant immature neutrophils), and the whole air collection of the exhaled breath of patients with AML. Cell cultures were grown in hermetically sealable glass culture flasks and the headspace above the culture was collected for analysis. The exhaled breath of patients with AML and the exhalant of healthy controls were collected in evacuated stainless steel canisters. Analysis of the samples was performed using gas chromatography. Cultured PBMCs were shown to reduce the concentration of acetaldehyde in the headspace relative to controls. Acetaldehyde in the headspace was 1.9±1 ppbv less than what was present in the media. Likewise hexanaldehyde was lower by 0.10±0.05 ppbv. Acute lymphocytic leukemia cells produced greater amounts of acetaldehyde and dimethyl sulfide relative to controls: 14±5 ppbv and 200±50 ppbv above controls. Healthy nuetrophils in culture produced a concentration of acetaldehyde of 4.5±5.4 ppbv above control and had a level of hexanaldehyde 80±60 pptv below controls. The HL60 cells produced 11±3 ppbv of acetaldehyde and 200±70 pptv of hexanladehyde. The exhaled breath of patients with AML contained levels of five VOCs that were significantly different from those found in the healthy controls: acetaldehyde, butane, carbon disulfide, carbonyl sulfide, and heptane. Carbon disulfide and butane were present in levels above the healthy controls and the ambient room concentrations: 15±17 pptv and 700±1,300 pptv. With the goal of diagnostic efficacy in mind it is hoped that the nascent VOC profile of leukemia discussed in this work may provide a foundation for future studies.
