Author: Randy Horwitz
Publisher:
ISBN: 9780530018584
Category : Science
Languages : en
Pages : 226
Book Description
Abstract: The purpose of this study was to understand the control of synthesis of Tdt at a molecular level, using a murine pre B-cell line inducible for the enzyme. FLEl-4, an Abelson virus transformed fetal liver cell line, when treated with dibutyryl cAMP and caffeine (or other phosphodiesterase inhibitors), shows a three- to tenfold induction of Tdt, as measured by immunoprecipitation of pulse labelled enzyme, activity assays, and steady-state northern blot. The study also revealed Tdt expression (mRNA induction) by a group of agents which inhibit DNA synthesis by various mechanisms. This finding implies that Tdt expression is unlinked to clonal proliferation of pre-B cells, and suggests a multistage model for B-cell differentiation and the generation of immunoglobulin somatic variation. At a molecular level, the agents that induce Tdt were also shown to increase intracellular cAMP levels shortly before Tdt transcription. Finally, an analysis of histone phosphorylation patterns in induced cells reveal a marked enhancement of histone HI phosphorylation, a finding which may be related to the induction event. Dissertation Discovery Company and the University of Florida are dedicated to making scholarly works more discoverable and accessible throughout the world. This dissertation, "The Regulation of Expression of Terminal Deoxynucleotidyl Transferase in an Inducible Murine Pre-B Cell Line" by Randy Jonathan Horwitz, was obtained from the University of Florida and is being sold with permission from the author. A free digital copy of this work may also be found in the university's institutional repository, the IR@UF. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation.
The Regulation of Expression of Terminal Deoxynucleotidyl Transferase in an Inducible Murine Pre-B Cell Line
Author: Randy Horwitz
Publisher:
ISBN: 9780530018584
Category : Science
Languages : en
Pages : 226
Book Description
Abstract: The purpose of this study was to understand the control of synthesis of Tdt at a molecular level, using a murine pre B-cell line inducible for the enzyme. FLEl-4, an Abelson virus transformed fetal liver cell line, when treated with dibutyryl cAMP and caffeine (or other phosphodiesterase inhibitors), shows a three- to tenfold induction of Tdt, as measured by immunoprecipitation of pulse labelled enzyme, activity assays, and steady-state northern blot. The study also revealed Tdt expression (mRNA induction) by a group of agents which inhibit DNA synthesis by various mechanisms. This finding implies that Tdt expression is unlinked to clonal proliferation of pre-B cells, and suggests a multistage model for B-cell differentiation and the generation of immunoglobulin somatic variation. At a molecular level, the agents that induce Tdt were also shown to increase intracellular cAMP levels shortly before Tdt transcription. Finally, an analysis of histone phosphorylation patterns in induced cells reveal a marked enhancement of histone HI phosphorylation, a finding which may be related to the induction event. Dissertation Discovery Company and the University of Florida are dedicated to making scholarly works more discoverable and accessible throughout the world. This dissertation, "The Regulation of Expression of Terminal Deoxynucleotidyl Transferase in an Inducible Murine Pre-B Cell Line" by Randy Jonathan Horwitz, was obtained from the University of Florida and is being sold with permission from the author. A free digital copy of this work may also be found in the university's institutional repository, the IR@UF. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation.
Publisher:
ISBN: 9780530018584
Category : Science
Languages : en
Pages : 226
Book Description
Abstract: The purpose of this study was to understand the control of synthesis of Tdt at a molecular level, using a murine pre B-cell line inducible for the enzyme. FLEl-4, an Abelson virus transformed fetal liver cell line, when treated with dibutyryl cAMP and caffeine (or other phosphodiesterase inhibitors), shows a three- to tenfold induction of Tdt, as measured by immunoprecipitation of pulse labelled enzyme, activity assays, and steady-state northern blot. The study also revealed Tdt expression (mRNA induction) by a group of agents which inhibit DNA synthesis by various mechanisms. This finding implies that Tdt expression is unlinked to clonal proliferation of pre-B cells, and suggests a multistage model for B-cell differentiation and the generation of immunoglobulin somatic variation. At a molecular level, the agents that induce Tdt were also shown to increase intracellular cAMP levels shortly before Tdt transcription. Finally, an analysis of histone phosphorylation patterns in induced cells reveal a marked enhancement of histone HI phosphorylation, a finding which may be related to the induction event. Dissertation Discovery Company and the University of Florida are dedicated to making scholarly works more discoverable and accessible throughout the world. This dissertation, "The Regulation of Expression of Terminal Deoxynucleotidyl Transferase in an Inducible Murine Pre-B Cell Line" by Randy Jonathan Horwitz, was obtained from the University of Florida and is being sold with permission from the author. A free digital copy of this work may also be found in the university's institutional repository, the IR@UF. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation.
The Regulation of Expression of Terminal Deoxynucleotidyl Transferase in an Inducible Murine Pre-B Cell Line
Author: Randy Jonathan Horwitz
Publisher:
ISBN:
Category :
Languages : en
Pages : 422
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 422
Book Description
Cumulated Index Medicus
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1160
Book Description
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1160
Book Description
Advances in Immunology
Author:
Publisher: Academic Press
ISBN: 0080578179
Category : Medical
Languages : en
Pages : 485
Book Description
Advances in Immunology
Publisher: Academic Press
ISBN: 0080578179
Category : Medical
Languages : en
Pages : 485
Book Description
Advances in Immunology
Annual Review of Immunology
Author:
Publisher:
ISBN:
Category : Cellular immunity
Languages : en
Pages : 764
Book Description
Publisher:
ISBN:
Category : Cellular immunity
Languages : en
Pages : 764
Book Description
Dissertation Abstracts International
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 548
Book Description
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 548
Book Description
Annual Review of Immunology
Author: William E. Paul
Publisher:
ISBN: 9780824330071
Category : Medical
Languages : en
Pages : 756
Book Description
Publisher:
ISBN: 9780824330071
Category : Medical
Languages : en
Pages : 756
Book Description
Index to American Doctoral Dissertations
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1252
Book Description
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1252
Book Description
Philosophical Transactions of the Royal Society of London
Author:
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 640
Book Description
Each issue of Transactions B is devoted to a specific area of the biological sciences, including clinical science. All papers are peer reviewed and edited to the highest standards. Published on the 29th of each month, Transactions B is essential reading for all biologists.
Publisher:
ISBN:
Category : Biology
Languages : en
Pages : 640
Book Description
Each issue of Transactions B is devoted to a specific area of the biological sciences, including clinical science. All papers are peer reviewed and edited to the highest standards. Published on the 29th of each month, Transactions B is essential reading for all biologists.
The Journal of Immunology
Author:
Publisher:
ISBN:
Category : Immunology
Languages : en
Pages : 1604
Book Description
Publisher:
ISBN:
Category : Immunology
Languages : en
Pages : 1604
Book Description