The Influences of Stretching and Decorin on the Structural and Mechanical Properties of Self-assembled Collagen Fibers PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Influences of Stretching and Decorin on the Structural and Mechanical Properties of Self-assembled Collagen Fibers PDF full book. Access full book title The Influences of Stretching and Decorin on the Structural and Mechanical Properties of Self-assembled Collagen Fibers by George Daniel Pins. Download full books in PDF and EPUB format.
Author: George Daniel Pins
Publisher:
ISBN:
Category :
Languages : en
Pages : 300
Get Book Here
Book Description
Author: George Daniel Pins
Publisher:
ISBN:
Category :
Languages : en
Pages : 300
Get Book Here
Book Description
Author: Peter Fratzl
Publisher: Springer Science & Business Media
ISBN: 0387739068
Category : Technology & Engineering
Languages : en
Pages : 516
Get Book Here
Book Description
Not only does this book provide a comprehensive review of current research advances in collagen structure and mechanics, it also explores this biological macromolecule’s many applications in biomaterials and tissue engineering. Readers gain an understanding of the structure and mechanical behavior of type I collagen and collagen-based tissues in vertebrates across all length scales, from the molecular (nano) to the organ (macro) level.
Author:
Publisher:
ISBN:
Category : Chemistry
Languages : en
Pages : 2566
Get Book Here
Book Description
Author: Naghmeh Rezaei
Publisher:
ISBN:
Category :
Languages : en
Pages : 97
Get Book Here
Book Description
Collagen is a key component of the extracellular matrix and is the most abundant protein in vertebrates. Collagen is found in almost every connective tissue of the body including skin, bone, tendon, cartilage, arteries and cornea, where it plays a crucial role in providing structural support. Collagen molecules self-assemble to form hierarchical structures, from single molecules to fibrils to fibers and tissues. Structural and mechanical changes at the molecular level may affect self-assembly of the molecules and the resulting tissue. Despite its significance, the mechanics of collagen and its flexibility at the molecular level remain contentious, and collagen has been variously described as a flexible polymer to a semi-rigid rod. In this thesis, I present my work developing and utilizing experimental and analytical tools to study the mechanical proprieties of molecular collagen. I carefully designed and controlled a wide variety of experimental conditions, such as different collagen types and sources, solution pH and salt concentrations, and analysed the results in search of potential reasons for inconsistency in reported results of collagen flexibility at the basic molecular level. Atomic force microscopy (AFM) imaging is used to study effect of environmental factors such as ionic strength and pH on molecular conformations and flexibility of single collagen molecules. In addition, molecular conformations of different types of collagen from different sources are compared using AFM imaging. I measure persistence length of collagen molecules, a measure of flexibility, arising due to the conformational sampling of collagen. My results link the bending energy of collagen molecules to how tightly the helix is wound. In order to analyse AFM images of collagen, I developed an image and statistical analysis algorithm, SmarTrace, optimized for my images of collagen. The program was validated using images of DNA with known persistence length, then applied to collagen molecules. Analysis of different types of collagen in two different solutions and type I collagen in solutions of different ionic strength and pH show that collagen's flexibility depends strongly on ionic strength and pH. In addition, it shows that different types of collagen show similar average conformational characteristics in a given solution environment. In addition, mechanical properties and force-response of single collagen and procollagen molecules are studied using optical tweezers. I discuss the challenges of stretching single collagen proteins, whose length is much less than the size of the microspheres used as manipulation handles, and show how instrumental design and biochemistry can be used to overcome these challenges. The result of this work is an improved understanding of the sensitivity of molecular flexibility, stability and response of collagen to environmental factors. This can shed light on identifying underlying mechanisms of collagen-related diseases as well as designing and producing improved engineered biomaterials with tunable properties.
Author: Amit Gefen
Publisher: Springer Science & Business Media
ISBN: 3642364829
Category : Technology & Engineering
Languages : en
Pages : 397
Get Book Here
Book Description
This book reviews the state-of-the-art in multiscale computer modeling, in terms of both accomplishments and challenges. The information in the book is particularly useful for biomedical engineers, medical physicists and researchers in systems biology, mathematical biology, micro-biomechanics and biomaterials who are interested in how to bridge between traditional biomedical engineering work at the organ and tissue scales, and the newer arenas of cellular and molecular bioengineering.
Author: Norbert Pallua
Publisher: Springer Science & Business Media
ISBN: 3642028241
Category : Medical
Languages : en
Pages : 632
Get Book Here
Book Description
Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. This abundantly illustrated and well-structured guide serves as a reference for all clinicians and researchers dealing with tissue engineering issues in their daily practice.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
Get Book Here
Book Description
Author: Jieling Zhu
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Finally, Chapter 6 describes the development, evaluation, and redesign of a Microsoft Excel-based activity where data analysis from contemporary research (published in Chapter 3) was adapted into a training exercise for students taking an introductory General Chemistry Laboratory course. This activity humanized the research enterprise for the undergraduate students and can serve as a model for future collaborations between research and instructional laboratories.
Author: Bela Suki
Publisher: Academic Press
ISBN: 0128226056
Category : Technology & Engineering
Languages : en
Pages : 284
Get Book Here
Book Description
Structure and Function of the Extracellular Matrix: A Multiscale Quantitative Approach introduces biomechanics and biophysics with applications to understand the biological function of the extracellular matrix in health and disease. A general multiscale approach is followed by investigating behavior from the scale of single molecules, through fibrils and fibers, to tissues of various organ systems. Through mathematical models and structural information, quantitative description of the extracellular matrix function is derived with tissue specific details. The book introduces the properties and organization of extracellular matrix components and quantitative models of the matrix, and guides the reader through predicting functional properties. This book integrates evolutionary biology with multiscale structure to quantitatively understand the function of the extracellular matrix. This approach allows a fresh look into normal functioning as well as the pathological alterations of the extracellular matrix. Professor Suki's book is written to be useful to undergraduates, graduate students, and researchers interested in the quantitative aspects of the extracellular matrix. Researchers working in mechanotransduction, respiratory and cardiovascular mechanics, and multiscale biomechanics of tendon, cartilage, skin, and bone may also be interested in this book. - Examines the evolutionary origins and consequences of the extracellular matrix - Delivers the first book to quantitatively treat the extracellular matrix as a multiscale system - Presents problems and a set of computational laboratory projects in various chapters to aid teaching and learning - Provides an introduction to the properties and organization of the extracellular matrix components
Author: Robert Mecham
Publisher: Springer Science & Business Media
ISBN: 3642165559
Category : Science
Languages : en
Pages : 429
Get Book Here
Book Description
Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.
