The Influence of Estrogen on Breast Cancer Cell Proliferation PDF Download
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Author: Virginia A. Spencer
Publisher:
ISBN:
Category :
Languages : en
Pages : 15
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Book Description
Estrogen affects both the cytoskeletal and chromatin structure of breast cancer cells. The treatment of breast cancer cells with 10 nM estradiol causes the estrogen receptor to recruit histone acetyltransferases to the promoter of the estrogen-responsive PS2 gene within 60 min, while exposure to estrogen for 72 h increases the levels of DNA-associated cytokeratins. To determine the effect of estrogen on regions downstream of estrogen- responsive promoters, quantitative PCR was performed on DNA isolated from chromatin immunoprecipitations using anti-acetylated 113 and H4 antibodies. Treatment with 10 nM estradiol induced a transient state of 113 and 114 acetylation along the PS2 promoter, exon 2, and exon 3, that was maximal after 60-120 min. After three hours, this hyperacetylation decreased to levels above those observed along the PS2 gene in untreated MCF-7 cells. To determine how estrogen influences DNA-associated cytokeratins, the levels of total, intermediate filament-assembled and DNA-associated cytokeratins were determined by Western Blotting and two dimension gel electrophoresis. The level of DNA-associated cytokeratins increased after 2 h of 10 nM estradiol treatment, while the levels of total and filament cytokeratins remained the same. This suggests that early estrogen exposure (i.e. 2 h) promotes interactions between cytokeratins and DNA.
Author: Virginia A. Spencer
Publisher:
ISBN:
Category :
Languages : en
Pages : 15
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Book Description
Estrogen affects both the cytoskeletal and chromatin structure of breast cancer cells. The treatment of breast cancer cells with 10 nM estradiol causes the estrogen receptor to recruit histone acetyltransferases to the promoter of the estrogen-responsive PS2 gene within 60 min, while exposure to estrogen for 72 h increases the levels of DNA-associated cytokeratins. To determine the effect of estrogen on regions downstream of estrogen- responsive promoters, quantitative PCR was performed on DNA isolated from chromatin immunoprecipitations using anti-acetylated 113 and H4 antibodies. Treatment with 10 nM estradiol induced a transient state of 113 and 114 acetylation along the PS2 promoter, exon 2, and exon 3, that was maximal after 60-120 min. After three hours, this hyperacetylation decreased to levels above those observed along the PS2 gene in untreated MCF-7 cells. To determine how estrogen influences DNA-associated cytokeratins, the levels of total, intermediate filament-assembled and DNA-associated cytokeratins were determined by Western Blotting and two dimension gel electrophoresis. The level of DNA-associated cytokeratins increased after 2 h of 10 nM estradiol treatment, while the levels of total and filament cytokeratins remained the same. This suggests that early estrogen exposure (i.e. 2 h) promotes interactions between cytokeratins and DNA.
Author: Michael Tsung-Ju Yang
Publisher:
ISBN:
Category :
Languages : en
Pages : 136
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Book Description
Author: Fritz F. Parl
Publisher: IOS Press
ISBN: 9780967335544
Category : Breast
Languages : en
Pages : 280
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Book Description
Estrogens have been implicated to play a role in the development of breast cancer. The purpose of this book is to provide a comprehensive analysis of experimental, clinical and epidemiological evidence in support of the carcinogenicity of estrogens.
Author: William L. McGuire
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 308
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Book Description
Author: Shyam Narayan Sundar
Publisher:
ISBN:
Category :
Languages : en
Pages : 438
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 23
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Book Description
Macrophage function can be regulated by breast cancer cells to sustain an inflammatory microenvironment that promotes cancer cell proliferation and survival. MCF-7 cells suppress IL-10 expression by co-cultured macrophages. Activated and resting THP-1 macrophages were able to induce SNAIL expression in MCF-7 cells. SNAIL has been implicated in the epithelial-mesenchymal transition which precedes metastasis. These observations suggest that tumor-associated macrophages may promote progression-related gene expression at discrete steps in the progression cascade. Both Faslodex(TradeMark) and tamoxifen were effective in up-regulating pigment epithelium-derived factor, a potent anti-angiogenic cytokine, and in down-regulating SNAIL in MCF-7 cells.
Author: Marc E. Lippman
Publisher: Springer Science & Business Media
ISBN: 1461539404
Category : Medical
Languages : en
Pages : 455
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Book Description
In Breast Cancer: Cellular and Molecular Biology [Kluwer Academic Pub lishers, 1988], we tried to present an introduction to the emerging basic studies on steroid receptors, oncogenes, and growth factors in the regulation of normal and malignant mammary epithelium. The response to this volume was superb, indicating a tremendous interest in basic growth regulatory mechanisms governing breast cancer and controlling its malignant progres sion. In the two years since its publication, much new and exciting in formation has been published and the full interplay of regulatory mechanisms is now beginning to emerge. We have divided this book into four sections that we hope will unify important concepts and help to crystallize areas of consensus and/or disagreement among a diverse group of basic and clinical scientists working on the disease. The first section is devoted to studies on oncogenes, antioncogenes, proliferation, and tumor prognosis. The first chapter, by Sunderland and McGuire, introduces the characteristics of breast cancer as studied by patho logists to establish prognostic outcome. Of particular interest is a new proto oncogene called HER-2 (or neu), which is rapidly becoming accepted as a valuable new tumor marker of poor prognosis. The second chapter, by Lee Bookstein and Lee, introduces the best known antioncogene, the retinoblas toma antioncogene, whose expression is sometimes lost in breast cancer. Malignant progression appears to be influenced by the balance of proto oncogene and antioncogene expression.
Author: W. R. Miller
Publisher: Landes Bioscience
ISBN:
Category : Medical
Languages : en
Pages : 234
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Book Description
Author: Virgil Craig Jordan
Publisher: Univ of Wisconsin Press
ISBN: 9780299104801
Category : Breast
Languages : en
Pages : 564
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Book Description
This book marks one of the achievements of the "Estrogen and Antiestrogen Action: Basic and Clinical Aspects" Wisconsin Clinical Cancer Center conference in June 1984. It is not intended to be a recount of the meeting proceedings but is, rather, a historical review of the development of this field of endeavor during the past 30 years. The chapters have been written by many of the leading experts in the field who were asked to recount the development of a particular area or laboratory idea in which they had been personally involved. The book is intended to provide both scientists and clinicians with a single-volume overview of both the basic principles of hormonal control of breast cancer and the recent clinical results from cooperative groups around the world.
Author: Jose Russo
Publisher: World Scientific
ISBN: 9813271213
Category : Medical
Languages : en
Pages : 688
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Book Description
This narrative of a cancer researcher spans a period in which biomedicine research has been so revolutionary. The educational background and socioeconomic circumstances of the author make the story unique, shedding light on many important intellectual achievements. The author also provides an insightful view on how decisions at the upper echelon of scientific institutions affect cancer researchers. The vivid account of scientific discovery and intellectual evolution provides a fine example for the next generation of cancer researchers.
