The Function of E. Coli Single Stranded DNA Binding Protein PDF Download
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Author: Jane M. Weisemann
Publisher:
ISBN:
Category : Amino acids
Languages : en
Pages : 140
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Book Description
Author: Jane M. Weisemann
Publisher:
ISBN:
Category : Amino acids
Languages : en
Pages : 140
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Book Description
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Mark R. Kelley
Publisher: Academic Press
ISBN: 0128035994
Category : Science
Languages : en
Pages : 466
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Book Description
DNA Repair and Cancer Therapy: Molecular Targets and Clinical Applications, Second Edition provides a comprehensive and timely reference that focuses on the translational and clinical use of DNA repair as a target area for the development of diagnostic biomarkers and the enhancement of cancer treatment. Experts on DNA repair proteins from all areas of cancer biology research take readers from bench research to new therapeutic approaches. This book provides a detailed discussion of combination therapies, in other words, how the inhibition of repair pathways can be coupled with chemotherapy, radiation, or DNA damaging drugs. Newer areas in this edition include the role of DNA repair in chemotherapy induced peripheral neuropathy, radiation DNA damage, Fanconi anemia cross-link repair, translesion DNA polymerases, BRCA1-BRCA2 pathway for HR and synthetic lethality, and mechanisms of resistance to clinical PARP inhibitors. - Provides a comprehensive overview of the basic and translational research in DNA repair as a cancer therapeutic target - Includes timely updates from the earlier edition, including Fanconi Anemia cross-link repair, translesion DNA polymerases, chemotherapy induced peripheral neuropathy, and many other new areas within DNA repair and cancer therapy - Saves academic, medical, and pharma researchers time by allowing them to quickly access the very latest details on DNA repair and cancer therapy - Assists researchers and research clinicians in understanding the importance of the breakthroughs that are contributing to advances in disease-specific research
Author: Donald E. Johnson
Publisher:
ISBN:
Category :
Languages : en
Pages : 242
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Author: Andrei Kuzminov
Publisher: Landes Bioscience
ISBN:
Category : Medical
Languages : en
Pages : 234
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 120
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Book Description
The DNA replication machinery (replisome) is a dynamic multi-protein complex that efficiently achieves the task of genome duplication. However, DNA replication is a vulnerable process to organisms due to the generation of single-stranded (ss) DNA intermediates. In addition, the DNA replication fork must overcome obstacles, such as template damage or frozen protein complexes, that threaten accurate and complete genome duplication. Cells encode specialized ssDNA-binding proteins (SSBs) to bind ssDNA and ensure the genomic template is maintained. The Escherichia coli (E. coli) SSB has also been found to interact with over a dozen proteins involved in the major genome maintenance pathways. The RNase HI enzyme specifically hydrolyzes the RNA in RNA:DNA hybrids and is involved in processing DNA replication lagging strand RNA primers as well as removing transcription-dependent R-loops that block replication fork progression. In this work I have confirmed and characterized the interaction between E. coli RNase HI and SSB. The protein complex is maintained through SSB’s highly conserved C-terminal tail (SSB-Ct). I located the SSB-Ct docking site on RNase HI and identified the binding pocket residues that are essential for maintaining the interaction in vitro. The RNase HI/SSB interaction stimulates RNase HI-mediated RNA:DNA hybrid hydrolysis by lowering the reaction’s Km, suggesting that SSB recruits RNase HI to its substrate. I have tested this hypothesis in cells by incorporating an SSB binding mutant identified from in vitro experiments, rnhAK60E, into the chromosome. The SSB interaction mediates the localization of RNase HI to the DNA replication fork but does not impact the role of RNase HI in lagging strand RNA primer processing. However, combining the point mutant with a Rep (accessory helicase localized to the replication fork) null mutant (rnhAK60E rep) renders cells sensitive to rich media and increases DNA damage. The rnhAK60E rep phenotype is influenced by mutations in RNA Polymerase likely arising from changes to levels of transcription-dependent R-loops. From the data presented in this thesis we have generated a model for the function of the RNase HI/SSB complex that provides a mechanism for localizing the cellular activity of the RNase HI enzyme.
Author: Fumio Hanaoka
Publisher: Springer
ISBN: 443155873X
Category : Science
Languages : en
Pages : 548
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Book Description
This book is a comprehensive review of the detailed molecular mechanisms of and functional crosstalk among the replication, recombination, and repair of DNA (collectively called the "3Rs") and the related processes, with special consciousness of their biological and clinical consequences. The 3Rs are fundamental molecular mechanisms for organisms to maintain and sometimes intentionally alter genetic information. DNA replication, recombination, and repair, individually, have been important subjects of molecular biology since its emergence, but we have recently become aware that the 3Rs are actually much more intimately related to one another than we used to realize. Furthermore, the 3R research fields have been growing even more interdisciplinary, with better understanding of molecular mechanisms underlying other important processes, such as chromosome structures and functions, cell cycle and checkpoints, transcriptional and epigenetic regulation, and so on. This book comprises 7 parts and 21 chapters: Part 1 (Chapters 1–3), DNA Replication; Part 2 (Chapters 4–6), DNA Recombination; Part 3 (Chapters 7–9), DNA Repair; Part 4 (Chapters 10–13), Genome Instability and Mutagenesis; Part 5 (Chapters 14–15), Chromosome Dynamics and Functions; Part 6 (Chapters 16–18), Cell Cycle and Checkpoints; Part 7 (Chapters 19–21), Interplay with Transcription and Epigenetic Regulation. This volume should attract the great interest of graduate students, postdoctoral fellows, and senior scientists in broad research fields of basic molecular biology, not only the core 3Rs, but also the various related fields (chromosome, cell cycle, transcription, epigenetics, and similar areas). Additionally, researchers in neurological sciences, developmental biology, immunology, evolutionary biology, and many other fields will find this book valuable.
Author: Duo Lu
Publisher:
ISBN:
Category :
Languages : en
Pages : 149
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Book Description
Author: Robert D. Wells
Publisher: Springer
ISBN: 9781461415299
Category : Science
Languages : en
Pages : 0
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Book Description
Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences.
Author:
Publisher: Academic Press
ISBN: 0128234695
Category : Science
Languages : en
Pages : 370
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Book Description
Viral Replication Enzymes and their Inhibitors Part A, Volume 49, the latest release in the Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in The Enzymes series
