The ELISA Guidebook PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The ELISA Guidebook PDF full book. Access full book title The ELISA Guidebook by John R. Crowther. Download full books in PDF and EPUB format.
Author: John R. Crowther
Publisher: Springer Science & Business Media
ISBN: 1592590497
Category : Science
Languages : en
Pages : 426
Get Book Here
Book Description
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author: John R. Crowther
Publisher: Springer Science & Business Media
ISBN: 1592590497
Category : Science
Languages : en
Pages : 426
Get Book Here
Book Description
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author: John R. Crowther
Publisher: Humana
ISBN: 9781617378843
Category : Medical
Languages : en
Pages : 0
Get Book Here
Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 1603273751
Category : Science
Languages : en
Pages : 1105
Get Book Here
Book Description
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Author: Elisa Amado
Publisher: Groundwood Books Ltd
ISBN: 155498288X
Category : Juvenile Fiction
Languages : en
Pages : 28
Get Book Here
Book Description
A picture book that captures a child’s discovery of the power of reading. Before he leaves for his first day of school, Chepito runs outside to play. He comes across all kinds of people in his neighborhood who are reading. “Why, why, why?” he sings, and they each have a different answer for him, whether it’s a man reading a newspaper, a young girl enjoying a comic, a couple of tourists consulting a guidebook or an archeologist studying hieroglyphics. Later that day, Chepito discovers the fun of reading for himself, and he even brings home a book to share with his little sister. Elisa Amado has written a perfect introduction to the pleasures and uses of the written word. Set in a delightfully retro world by illustrator Manuel Monroy, this book is a true celebration of reading. Correlates to the Common Core State Standards in English Language Arts: CCSS.ELA-LITERACY.RL.K.4 Ask and answer questions about unknown words in a text. CCSS.ELA-LITERACY.RL.1.2 Retell stories, including key details, and demonstrate understanding of their central message or lesson. CCSS.ELA-LITERACY.RL.2.4 Describe how words and phrases (e.g., regular beats, alliteration, rhymes, repeated lines) supply rhythm and meaning in a story, poem, or song.
Author: Elisa Velasquez
Publisher: Createspace Independent Publishing Platform
ISBN: 9781541329089
Category :
Languages : en
Pages :
Get Book Here
Book Description
Excerpts from Elisa's personal diary of her conversations with God. I'm always writing, God is constantly talking and I'm always writing. This book is uniquely wonderful and penned with my heart. I love sharing what I write and the words that God whispers in my ear. It's important to build people up and inspire and even correct. This diary does just that. Elisa spraks to your spirit heart and delivers God's Messages purely and honest. Listen to God's word. Elisa's Diary are excerpts from her daily personal God diary. Keeping a diary is most rewarding and memorable. Your thoughts are probably the best legacies to leave behind on earth.
Author: Dr. Elisa Port
Publisher: Ballantine Books
ISBN: 1101883146
Category : Health & Fitness
Languages : en
Pages : 322
Get Book Here
Book Description
From an expert in the field comes the definitive guide to managing breast cancer in the information age—a comprehensive resource for diagnosis, treatment, and peace of mind. The breast cancer cure rate is at an all-time high, and so is the information, to say nothing of the misinformation, available to patients and their families. Online searches can lead to unreliable sources, leaving even the most resilient patient feeling uneasy and uncertain about her diagnosis, treatment options, doctors, side effects, and recovery. Adding to a patient’s anxiety is input from well-meaning friends and family, with stories, worries, and opinions to share, sometimes without knowing the details of her particular case, when in reality breast cancer treatment has gone well beyond a “one size fits all” approach. Elisa Port, MD, FACS, chief of breast surgery at The Mount Sinai Hospital and co-director of the Dubin Breast Center in Manhattan, offers an optimistic antidote to the ocean of Web data on screening, diagnosis, prognosis, and treatment. Inside you’ll discover • the various scenarios when mammograms indicate the need for a biopsy • the questions to ask about surgery, chemotherapy, radiation, and breast reconstruction • the important things to look for when deciding where to get care • the key to deciphering complicated pathology reports and avoiding confusion • the facts on genetic testing and the breast cancer genes: BRCA-1 and BRCA-2 • the best resources and advice for those supporting someone with breast cancer From innovations in breast cancer screening and evaluating results to post-treatment medications and living as a breast cancer survivor, Dr. Elisa Port describes every possible test and every type of doctor visit, providing a comprehensive, empathetic guide that every newly diagnosed woman (and her family) will want to have at her side. Praise for The New Generation Breast Cancer Book “One book you need . . . If you’re considering your options for treatment or know someone who is, this step-by-step guide, The New Generation Breast Cancer Book, is essential reading.”—InStyle “Elisa Port, M.D., is the doctor every patient deserves: brilliant and compassionate. Her book will be a sanity saver and, quite possibly, a life saver.”—Geralyn Lucas, author of Why I Wore Lipstick to My Mastectomy “As up-to-date as one can get, with lots to offer people facing a cancer diagnosis or hoping to support someone with the disease.”—Library Journal (starred review) “The New Generation Breast Cancer Book helps you sort through all the information you’ve gathered, clarify the terminology, consider the options, and make the right decisions for your unique case.”—Edie Falco “A lifeline for many women in need of today’s most up-to-date choices for treatment . . . Everyone should read this book for themselves, their mothers, grandmothers, daughters, and friends.”—Kara DioGuardi, Grammy-nominated songwriter, music executive, and Arthouse Entertainment co-founder “The book is teeming with easy-to-understand medical explanations, tips, takeaways, and pro-and-con discussions of various courses of action. Port also includes two extremely useful appendices that respectively take on common myths and answer questions frequently asked by friends and family. This is a vital read that will empower men and women alike.”—Publishers Weekly
Author: Donald Bloxham
Publisher: OUP Oxford
ISBN: 0191613614
Category : History
Languages : en
Pages : 2698
Get Book Here
Book Description
Genocide has scarred human societies since Antiquity. In the modern era, genocide has been a global phenomenon: from massacres in colonial America, Africa, and Australia to the Holocaust of European Jewry and mass death in Maoist China. In recent years, the discipline of 'genocide studies' has developed to offer analysis and comprehension. The Oxford Handbook of Genocide Studies is the first book to subject both genocide and the young discipline it has spawned to systematic, in-depth investigation. Thirty-four renowned experts study genocide through the ages by taking regional, thematic, and disciplinary-specific approaches. Chapters examine secessionist and political genocides in modern Asia. Others treat the violent dynamics of European colonialism in Africa, the complex ethnic geography of the Great Lakes region, and the structural instability of the continent's northern horn. South and North America receive detailed coverage, as do the Ottoman Empire, Nazi-occupied Europe, and post-communist Eastern Europe. Sustained attention is paid to themes like gender, memory, the state, culture, ethnic cleansing, military intervention, the United Nations, and prosecutions. The work is multi-disciplinary, featuring the work of historians, anthropologists, lawyers, political scientists, sociologists, and philosophers. Uniquely combining empirical reconstruction and conceptual analysis, this Handbook presents and analyses regions of genocide and the entire field of 'genocide studies' in one substantial volume.
Author: Elisa Camahort Page
Publisher: Ten Speed Press
ISBN: 0399581650
Category : Political Science
Languages : en
Pages : 209
Get Book Here
Book Description
A handbook for effective activism, advocacy, and social justice for people of all ages and backgrounds. Are you ready to take action and make your voice heard, but don't know how to go about it? This hands-on, hit-the-ground-running guide delivers lessons on practical tactics for navigating and protecting one's personal democracy in a gridlocked, heavily surveilled, and politically volatile country. If you want to start making a difference but don’t know what to do next, Road Map for Revolutionaries provides the resources needed to help you feel safer, more empowered, invested in, and intrinsic to the American experiment. The book addresses timely topics such as staying safe at protests, supporting marginalized communities, online privacy, and how to keep up the fight for the long term, breaking down key issues and outlining action steps for local, state, and federal levels of government.
Author: Paolo Palange
Publisher: European Respiratory Society
ISBN: 1849840806
Category : Medical
Languages : en
Pages : 891
Get Book Here
Book Description
The European Respiratory Society (ERS) Handbook of Respiratory Medicine, now in its third edition, is a concise, compact and easy-to-read guide to each of the key areas in respiratory medicine. Its 20 sections, written by clinicians and researchers at the forefront of the field, explain the structure and function of the respiratory system, its disorders and how to treat them. The Handbook is a must-have for anyone who intends to remain up to date in the field, and to have within arm's reach a reference that covers everything from the basics to the latest developments in respiratory medicine.
Author: Tim Wing Yam
Publisher: John Wiley & Sons
ISBN: 1119187060
Category : Science
Languages : en
Pages : 2363
Get Book Here
Book Description
Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about: Culture media and vessels Techniques and procedures for both orchids which were previously cultured and for those which were not Plant hormones and growth regulators Media components Methods for tissue decontamination Historical information Procedures for the cultivation for plantlets which have been removed from flasks Sources of light and illumination methods Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids.
