The Delivery of Biologicals Across Endosomal Membranes by PH-responsive Polymers PDF Download
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Author: Franziska Hausig-Punke
Publisher:
ISBN:
Category :
Languages : de
Pages : 0
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Book Description
The efficient and safe delivery of complex biological agents offers great potential for the challenges of our time. Polymers offer considerable advantages in this regard, not only because of their low risk for immunogenic reactions, their comparatively inexpensive large-scale production and their long shelf life. The biological properties of organisms, organs, tissues and cells determine the design of safe and efficient delivery systems and should be considered as inspiration for new attempts to create effective materials. In addition to the balance between hydrophobicity and hydrophilicity, the degree of charge of a nanocarrier is also critical for efficient delivery and overcoming the endosome. Cationic charges do not only enhance cellular uptake but can also mediate a crossing of the endosomal barrier due to membrane interactions. The introduction of hydrophobic or even lipophilic components can further significantly increase membrane interactions. Besides the interaction with the cell membrane, it is important to consider that both the degree of charge and the hydrophobic-hydrophilic balance also affect the packaging and protection of sensitive biological cargo such as genetic material. However, cationic charges and hydrophobic components introduce some disadvantages, as electrostatic and hydrophobic interactions may disrupt the integrity of other cellular membranes, triggering toxic effects. Fine adjustment of the degree of charge and the hydrophobic-hydrophilic balance offer the possibility of balancing the requirements for a safe, yet efficient delivery process. In the present dissertation, various polymer systems with adjustable charge density and hydrophobic-hydrophilic balance were investigated with respect to their delivery efficiencies. A particular focus was set on the key step in delivery, the endosomal release.
Author: Franziska Hausig-Punke
Publisher:
ISBN:
Category :
Languages : de
Pages : 0
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Book Description
The efficient and safe delivery of complex biological agents offers great potential for the challenges of our time. Polymers offer considerable advantages in this regard, not only because of their low risk for immunogenic reactions, their comparatively inexpensive large-scale production and their long shelf life. The biological properties of organisms, organs, tissues and cells determine the design of safe and efficient delivery systems and should be considered as inspiration for new attempts to create effective materials. In addition to the balance between hydrophobicity and hydrophilicity, the degree of charge of a nanocarrier is also critical for efficient delivery and overcoming the endosome. Cationic charges do not only enhance cellular uptake but can also mediate a crossing of the endosomal barrier due to membrane interactions. The introduction of hydrophobic or even lipophilic components can further significantly increase membrane interactions. Besides the interaction with the cell membrane, it is important to consider that both the degree of charge and the hydrophobic-hydrophilic balance also affect the packaging and protection of sensitive biological cargo such as genetic material. However, cationic charges and hydrophobic components introduce some disadvantages, as electrostatic and hydrophobic interactions may disrupt the integrity of other cellular membranes, triggering toxic effects. Fine adjustment of the degree of charge and the hydrophobic-hydrophilic balance offer the possibility of balancing the requirements for a safe, yet efficient delivery process. In the present dissertation, various polymer systems with adjustable charge density and hydrophobic-hydrophilic balance were investigated with respect to their delivery efficiencies. A particular focus was set on the key step in delivery, the endosomal release.
Author: David James Peeler
Publisher:
ISBN:
Category :
Languages : en
Pages : 176
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Book Description
Biomacromolecules such as nucleic acids and peptides have great potential as therapeutics but must overcome many challenging biological barriers to succeed in the clinic. In this work, we present several investigations of stimulus-responsive polymers developed to navigate both extracellular and intracellular barriers to biologic drug delivery. We begin with a review of past applications of pH-responsive chemistries in nucleic acid delivery (Chapter 1). After screening a panel of lytic peptides in a pH-sensitive polymer conjugate system that mediates endosomal escape of plasmid cargo (Chapter 2), we evaluate the most promising gene delivery vector in a mouse model of traumatic brain injury (Chapter 3). Our efforts to develop engineered stem cells transplants as alternatives to gene delivery are then discussed (Chapter 4). Finally, we adapt our pH-responsive polymer for the delivery of peptide vaccine cargo (Chapter 5) and detail future directions to further improve antigen and adjuvant delivery with polymers (Chapter 6).
Author: William R Wagner
Publisher: Academic Press
ISBN: 0128161388
Category : Technology & Engineering
Languages : en
Pages : 1612
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Book Description
The revised edition of the renowned and bestselling title is the most comprehensive single text on all aspects of biomaterials science from principles to applications. Biomaterials Science, fourth edition, provides a balanced, insightful approach to both the learning of the science and technology of biomaterials and acts as the key reference for practitioners who are involved in the applications of materials in medicine.This new edition incorporates key updates to reflect the latest relevant research in the field, particularly in the applications section, which includes the latest in topics such as nanotechnology, robotic implantation, and biomaterials utilized in cancer research detection and therapy. Other additions include regenerative engineering, 3D printing, personalized medicine and organs on a chip. Translation from the lab to commercial products is emphasized with new content dedicated to medical device development, global issues related to translation, and issues of quality assurance and reimbursement. In response to customer feedback, the new edition also features consolidation of redundant material to ensure clarity and focus. Biomaterials Science, 4th edition is an important update to the best-selling text, vital to the biomaterials’ community. The most comprehensive coverage of principles and applications of all classes of biomaterials Edited and contributed by the best-known figures in the biomaterials field today; fully endorsed and supported by the Society for Biomaterials Fully revised and updated to address issues of translation, nanotechnology, additive manufacturing, organs on chip, precision medicine and much more. Online chapter exercises available for most chapters
Author: Paul Ducheyne
Publisher: Elsevier
ISBN: 0080552943
Category : Technology & Engineering
Languages : en
Pages : 3659
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Book Description
Comprehensive Biomaterials brings together the myriad facets of biomaterials into one, major series of six edited volumes that would cover the field of biomaterials in a major, extensive fashion: Volume 1: Metallic, Ceramic and Polymeric Biomaterials Volume 2: Biologically Inspired and Biomolecular Materials Volume 3: Methods of Analysis Volume 4: Biocompatibility, Surface Engineering, and Delivery Of Drugs, Genes and Other Molecules Volume 5: Tissue and Organ Engineering Volume 6: Biomaterials and Clinical Use Experts from around the world in hundreds of related biomaterials areas have contributed to this publication, resulting in a continuum of rich information appropriate for many audiences. The work addresses the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, and strategic insights for those entering and operational in diverse biomaterials applications, research and development, regulatory management, and commercial aspects. From the outset, the goal was to review materials in the context of medical devices and tissue properties, biocompatibility and surface analysis, tissue engineering and controlled release. It was also the intent both, to focus on material properties from the perspectives of therapeutic and diagnostic use, and to address questions relevant to state-of-the-art research endeavors. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance as well as future prospects Presents appropriate analytical methods and testing procedures in addition to potential device applications Provides strategic insights for those working on diverse application areas such as R&D, regulatory management, and commercial development
Author: Deepa H. Patel
Publisher: CRC Press
ISBN: 1000517594
Category : Science
Languages : en
Pages : 228
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Book Description
This volume, Bioresponsive Polymers: Design and Application in Drug Delivery, focuses on recent advancements in bioresponsive polymers and their design, characterization, and applications in varied fields, such as drug delivery and gene delivery. It looks at several carriers for drug delivery and biological molecules using different bioresponsive polymers. To address the many difficulties in existing dosage forms, this book provides information on recent developments to overcome drawbacks of conventional forms of the drug delivery. The chapters cover most areas of bioresponsive polymers, starting with a basic introduction to bioresponsive polymers, followed by chapters on design, characterization, and mechanism of bioresponsive polymers; and applications of drug and gene delivery using bioresponsive polymers via oral, topical, nasal, ocular, and parenteral methods. The book also reviews recent advancements in bioresponsive polymers and advanced applications, such as engineering particulate moieties, biomedical applications, hydrogels as emerging therapy, and electrochemical responses, bioresponsive nanoparticles, and bioresponsive hydrogels.
Author: Lara Scheherazade Milane
Publisher: CRC Press
ISBN: 1000505936
Category : Science
Languages : en
Pages : 511
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Book Description
We have surpassed the omics era and are truly in the Age of Molecular Therapeutics. The fast-paced development of SARS-CoV-2 vaccines, such as the mRNA vaccines encoding the viral spike protein, demonstrated the need for and capability of molecular therapy and nanotechnology-based solutions for drug delivery. In record speed, the SARS-CoV-2 viral RNA genome was sequenced and shared with the scientific community, allowing the rapid design of molecular therapeutics. The mRNA vaccines exploit the host cell endoplasmic reticulum to produce viral spike proteins for antigen presentation and recognition by the innate and adaptive immune system. Lipid nanoparticles enable the delivery of the fragile, degradation-sensitive nucleic acid payloads. Molecular-based therapeutics and nanotechnology solutions continue to drive the scientific and medical response to the COVID-19 pandemic as new mRNA, DNA, and protein-based vaccines are developed and approved and the emergency use approved vaccines are rapidly manufactured and distributed throughout the globe. The need for molecular therapies and drug delivery solutions is clear, and as these therapies progress and become more specialized there will be important advancements in organelle targeting. For example, using organelle targeting to direct lipid nanoparticles with mRNA payloads to the endoplasmic reticulum would increase the efficacy of mRNA vaccines, reducing the required dose and therefore the biomanufacturing demand. Likewise, improving the delivery of DNA therapeutics to the nucleus would improve efficacy. Organelles and molecules have always been drug targets, but until recently we have not had the tools or capability to design and develop such highly specific therapeutics. Organelle targeting has far-reaching implications. For example, mitochondria are central to both energy production and intrinsic apoptosis. Effectively targeting and manipulating mitochondria has therapeutic applications for diseases such as myopathies, cancer, neurodegeneration, progerias, diabetes, and the natural aging process. The SARS-CoV-2 vaccines that exploit the endoplasmic reticulum (for mRNA vaccines) and the nucleic translational process (DNA vaccines) attest to the need for organelle and molecular therapeutics. This book covers the status, demand, and future of organelle- and molecularly targeted therapeutics that are critical to the advancement of modern medicine. Organelle and molecular targeting is the drug design and drug delivery approach of today and the future; understanding this approach is essential for students, scientists, and clinicians contributing to modern medicine.
Author:
Publisher:
ISBN:
Category : Chemical engineering
Languages : en
Pages : 1000
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Book Description
Author: Jorge Coelho
Publisher: Springer Science & Business Media
ISBN: 9400760108
Category : Medical
Languages : en
Pages : 433
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Book Description
This book is part of a series dedicated to recent advances on preventive, predictive and personalised medicine (PPPM). It focuses on the theme of “Drug delivery systems: advanced technologies potentially applicable in personalised treatments”. The critical topics involving the development and preparation of effective drug delivery systems, such as: polymers available, self-assembly, nanotechnology, pharmaceutical formulations, three dimensional structures, molecular modeling, tailor-made solutions and technological tendencies, are carefully discussed. The understanding of these areas constitutes a paramount route to establish personalised and effective solutions for specific diseases and individuals.
Author: Nandakumar Kalarikkal
Publisher: CRC Press
ISBN: 1498726429
Category : Medical
Languages : en
Pages : 548
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Book Description
This book focuses on the recent advances in nanomedicine and tissue engineering. It outlines the basic tools and novel approaches that are becoming available in nanomedicine and tissue engineering and considers the full range of nanomedical applications which employ molecular nanotechnology inside the human body, from the perspective of a future pr
Author: Ambikanandan Misra
Publisher: Elsevier
ISBN: 0128226684
Category : Technology & Engineering
Languages : en
Pages : 594
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Book Description
Applications of Polymers in Drug Delivery, Second Edition, provides a comprehensive resource for anyone looking to understand how polymeric materials can be applied to current, new, and emerging drug delivery applications. Polymers play a crucial role in modulating drug delivery and have been fundamental in the successful development of many novel drug delivery systems. This book describes the development of polymeric systems, ranging from conventional dosage forms to the most recent smart systems. Regulatory and intellectual property aspects as well as the clinical applicability of polymeric drug delivery systems are also discussed. The chapters are organized by specific delivery route, offering methodical and detailed coverage throughout. This second edition has been thoroughly revised to include the latest developments in the field. This is an essential book for researchers, scientists, and advanced students, in polymer science, drug delivery, pharmacology/pharmaceuticals, materials science, tissue engineering, nanomedicine, chemistry, and biology. In industry, this book supports scientists, R&D, and other professionals, working on polymers for drug delivery applications. - Explains how polymers can be prepared and utilized for all major drug delivery routes - Presents the latest advances, including drug targeting, polymeric micelles and polymersomes, and the delivery of biologicals and nucleic acid therapeutics - Includes appendices with in-depth information on pharmaceutical properties of polymers and regulatory aspects
