Structural Studies Involving the Escherichia Coli Catabolite Gene Activator Protein PDF Download
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Author: Jonathan Michael Passner
Publisher:
ISBN:
Category :
Languages : en
Pages : 204
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Author: Jonathan Michael Passner
Publisher:
ISBN:
Category :
Languages : en
Pages : 204
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Author: Richard High Ebright
Publisher:
ISBN:
Category : Allosteric proteins
Languages : en
Pages : 630
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Author: Ramya Rangesh Rao
Publisher:
ISBN:
Category : Cobalt
Languages : en
Pages : 37
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The Escherichia Coli Catabolite Activator Protein (CAP) activates DNA transcription at more than a hundred promoters. Cytidine Repressor (CytR), in conjunction with two CAP dimers, acts as a repressor of DNA transcription at the deoP2 promoter. In the first part of this work, we describe a method by which a (CAP)2-CytR-DNA complex can be prepared for structural studies. In the second part of this project, we describe the crystallization of what was initially intended to be a (CAP)2-DNA complex in order to study the effects of two CAP dimers on transcription at an artificially constructed promoter containing tandem CAP binding sites. Upon structure determination of the crystal, we observed that while there was no DNA present, the protein had bound multiple Co2+ and SO2-/4 ligands. We provide an analysis of the crystal structure and present a possible explanation for the absence of DNA in the structure.
Author: Thomas A. Steitz
Publisher: CUP Archive
ISBN: 9780521414890
Category : Science
Languages : en
Pages : 108
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In this 1993 text, Nobel Prize winner Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response.
Author: Angelo Hermanjie Gunasekera
Publisher:
ISBN:
Category :
Languages : en
Pages : 476
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Author: Jennifer Marie Thorn
Publisher:
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Category :
Languages : en
Pages :
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Author: K. Shimizu
Publisher: Elsevier
ISBN: 1908818204
Category : Science
Languages : en
Pages : 497
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The metabolic regulation of a cell system is of critical importance in systems biology, and a robust model of these mechanisms is essential in predicting the effects on the metabolism of both the culture environment and the knockout of specific genes. Bacterial cellular metabolic systems focuses on this highly topical subject in relation to culture environment and provides a detailed analysis from gene level to metabolic level regulation, as well as offering a discussion of the most recent modelling approaches. The book begins with an introduction to metabolic mechanisms and to the metabolic regulation of a cell, before moving on to discussing the action of global regulators in response to a specific culture environment. The second half of the book examines conventional flux balance analysis and its applications, 13C-metabolic flux analysis, and the effect of a specific gene knockout on the metabolism. - Comprehensive account of metabolic regulation via global regulators in response to changes in the culture environment - Basic formulation of 13C-metabolic flux analysis based on 13C-labelling experiments - Systems biology approach for the modelling and computer simulation of the main metabolic pathways of a cell system
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 848
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Author: Patrick Elvin Clarke
Publisher:
ISBN:
Category : Salmonella typhimurium
Languages : en
Pages : 364
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