Structural and Functional Analysis of the Clamp Loader in the E. Coli DNA Polymerase III Holoenzyme PDF Download
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Author: Hui Xiao
Publisher:
ISBN:
Category :
Languages : en
Pages : 328
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Author: Hui Xiao
Publisher:
ISBN:
Category :
Languages : en
Pages : 328
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Author: Maria Armenia Carrondo
Publisher: Springer
ISBN: 9400725302
Category : Science
Languages : en
Pages : 213
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Book Description
This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
Author: Stuart MacNeill
Publisher: Springer Science & Business Media
ISBN: 9400745729
Category : Medical
Languages : en
Pages : 344
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Book Description
High-fidelity chromosomal DNA replication underpins all life on the planet. In humans, there are clear links between chromosome replication defects and genome instability, genetic disease and cancer, making a detailed understanding of the molecular mechanisms of genome duplication vital for future advances in diagnosis and treatment. Building on recent exciting advances in protein structure determination, the book will take the reader on a guided journey through the intricate molecular machinery of eukaryotic chromosome replication and provide an invaluable source of information, ideas and inspiration for all those with an interest in chromosome replication, whether from a basic science, translational biology and medical research perspective.
Author: Ulrich Hubscher
Publisher: World Scientific
ISBN: 9814465453
Category : Medical
Languages : en
Pages : 338
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Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based diagnostics and in techniques to analyze ancient and otherwise damaged DNA. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. We have recently witnessed the discovery of an abundance of novel DNA polymerases in viruses, bacteria, archaea and eukaryotes with specialized properties whose physiological functions are only beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes. It is intended for a wide audience from basic scientists, to diagnostic laboratories and to clinicians who seek a better understanding of these fascinating enzymes.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 768
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Author: Noah Ravede
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
In Escherichia coli, DNA replication is a complex process that involves the DNA polymerase III holoenzyme, which is made up of the DNA polymerase III core, the clamp loader, and the beta clamp. The beta clamp is essential for stabilizing the replication proteins, such as DNA polymerase III, onto DNA to allow it to carry out replication. The beta clamp is loaded onto DNA by a protein complex known as the clamp loader. SSB (single-stranded DNA binding protein) also coats the single-stranded DNA during the replication process and is known to interact with the clamp loader. DNA replicates in the 5' to 3'direction, so it is imperative that the beta clamp is loaded onto the correct end of DNA. However, it is unknown how the clamp loader can recognize the correct end of DNA for this process. Previous structural studies have identified a residue in the delta subunit of the clamp loader, tyrosine 316, that potentially recognizes the 3' end of the primer-template (p/t) junction and separates the strands. The goal of this study is to understand the importance of both the SSB-clamp loader interaction and the Y316A residue of the clamp loader in proper clamp loader orientation on DNA.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1828
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Author: Melvin DePamphilis
Publisher: Garland Science
ISBN: 1136738223
Category : Science
Languages : en
Pages : 736
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Book Description
Genome Duplication provides a comprehensive and readable overview of the underlying principles that govern genome duplication in all forms of life, from the simplest cell to the most complex multicellular organism. Using examples from the three domains of life - bacteria, archaea, and eukarya - Genome Duplication shows how all living organisms store their genome as DNA and how they all use the same evolutionary-conserved mechanism to duplicate it: semi-conservative DNA replication by the replication fork. The text shows how the replication fork determines where organisms begin genome duplication, how they produce a complete copy of their genome each time a cell divides, and how they link genome duplication to cell division. Genome Duplication explains how mistakes in genome duplication are associated with genetic disorders and cancer, and how understanding genome duplication, its regulation, and how the mechanisms differ between different forms of life, is critical to the understanding and treatment of human disease.
Author: Proceedings of the National Academy of Sciences
Publisher: National Academies Press
ISBN: 030907424X
Category : Science
Languages : en
Pages : 303
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Book Description
There has been a sea change in how we view genetic recombination. When germ cells are produced in higher organisms, genetic recombination assures the proper segregation of like chromosomes. In the course of that process, called meiosis, recombination not only assures segregation of one chromosome of each type to progeny germ cells, but also further shuffles the genetic deck, contributing to the unique inheritance of individuals. In a nutshell, that is the classical view of recombination. We have also known for many years that in bacteria recombination plays a role in horizontal gene transfer and in replication itself, the latter by establishing some of the replication forks that are the structural scaffolds for copying DNA. In recent years, however, we have become increasingly aware that replication, which normally starts without any help from recombination, is a vulnerable process that frequently leads to broken DNA. The enzymes of recombination play a vital role in the repair of those breaks. The recombination enzymes can function via several different pathways that mediate the repair of breaks, as well as restoration of replication forks that are stalled by other kinds of damage to DNA. Thus, to the classical view of recombination as an engine of inheritance we must add the view of recombination as a vital housekeeping function that repairs breaks suffered in the course of replication. We have also known for many years that genomic instability--including mutations, chromosomal rearrangements, and aneuploidy--is a hallmark of cancer cells. Although genomic instability has many contributing causes, including faulty replication, there are many indications that recombination, faulty or not, contributes to genome instability and cancer as well. The (Nas colloquium) Links Between Recombination and Replication: Vital Roles of Recombination was convened to broaden awareness of this evolving area of research. Papers generated by this colloquium are published here. To encourage the desired interactions of specialists, we invited some contributions that deal only with recombination or replication in addition to contributions on the central thesis of functional links between recombination and replication. To aid the nonspecialist and specialist alike, we open the set of papers with a historical overview by Michael Cox and we close the set with a commentary on the meeting and the field by Andrei Kuzminov.
Author: Slobodan Jergić
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 448
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Book Description
