Sperm Cryopreservation of Red Tilapia Fish PDF Download
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Author: Asmad Kari
Publisher: LAP Lambert Academic Publishing
ISBN: 9783848403363
Category :
Languages : en
Pages : 120
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Book Description
Cryopreservation of fish sperm is a valuable method for restoration of endangered species as well as serving as complementary technique of reproductive manipulation for genetic improvement. In the cryopreservation of sperm, exposure to cryoprotectant prior to freezing is an important factor for many species. Therefore, the present study was conducted to develop an optimal freezing rate in the cryopreservation protocol of red tilapia fish sperm. The results obtained from the present study showed that the main factors that influenced the quality of sperm used in cryopreservation were molarity of glycerol in extender, equilibration time, vapour temperature and individual fish. However, in an attempt to develop an efficient and routine cryopreservation technique of fish sperm, more research needs to be conducted.
Author: Asmad Kari
Publisher: LAP Lambert Academic Publishing
ISBN: 9783848403363
Category :
Languages : en
Pages : 120
Get Book Here
Book Description
Cryopreservation of fish sperm is a valuable method for restoration of endangered species as well as serving as complementary technique of reproductive manipulation for genetic improvement. In the cryopreservation of sperm, exposure to cryoprotectant prior to freezing is an important factor for many species. Therefore, the present study was conducted to develop an optimal freezing rate in the cryopreservation protocol of red tilapia fish sperm. The results obtained from the present study showed that the main factors that influenced the quality of sperm used in cryopreservation were molarity of glycerol in extender, equilibration time, vapour temperature and individual fish. However, in an attempt to develop an efficient and routine cryopreservation technique of fish sperm, more research needs to be conducted.
Author: Elise V. Hansen
Publisher:
ISBN:
Category : Fishes
Languages : en
Pages : 0
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Book Description
Conservation efforts are implemented worldwide in an attempt to mitigate the decline in biodiversity experienced today. As of 2021, over 3000 species of fish worldwide have been classified as critically endangered, endangered, or vulnerable.Primary contributors include climate change, pollution, habitat loss, over fishing, and invasive species. These issues are not being combated fast enough to save species from extinction. Thus, it is vital to develop complementary approaches to conserve biodiversity. An area of research under development is cryopreservation of fish sperm.Sperm cryopreservation provides an excellent method for species conservation by allowing for long-term storage of genetic material without risk of deterioration; therefore,providing a safeguard against species collapse. Unfortunately, cryopreservation is difficult to standardize across fish species due to its physiological complexity as well as biological differences of spermatozoa across species that influence their tolerance to the freeze/thaw process. Thus, species-specific methods must be developed. Blue tilapia(Oreochromis aureus) are of particular interest due to their importance as a food source for humans, excellent adaptability to being in culture, they are easy to breed, reproduce quickly, and tolerate high stocking densities. However, there is limited research on the cryopreservation of blue tilapia sperm, as the focus often lies on Nile and Mozambique tilapia. Therefore, I investigated an optimized cryopreservation protocol for blue tilapia sperm by testing combinations of three different cryoprotectants: dimethyl sulfoxide(DMSO), methanol (MeOH), and glycerol (Gly), with three different slow cooling rates: -1°C/min, -5°C/min, and -10°C/min. After thawing, viability of the protocols was assessed through spermatozoa motility and swimming kinematics as well as cell membrane damage using Trypan Blue membrane stain and a lactate dehydrogenase (LDH) enzyme assay on seminal plasma. Results indicate that DMSO at -5°C/min yielded consistently better outcomes than other treatments, however, due to limited overall success, further investigation is required to achieve the most effective protocol. Finally, percent motilityanalysis and Trypan Blue staining provided the most reliable results while LDH activity and swimming kinematics were less conclusive as viability measures.
Author: Asmad Kari
Publisher:
ISBN:
Category : Cryopreservation of organs, tissues, etc
Languages : en
Pages : 228
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Book Description
Author: Judith Betsy
Publisher: Springer Nature
ISBN: 981154025X
Category : Science
Languages : en
Pages : 356
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Book Description
Understanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation.The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists.This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.
Author: Yusuf Bozkurt
Publisher:
ISBN:
Category : Science
Languages : en
Pages :
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Book Description
The main aim of this study is to determine the effect of the straw volume (0.25 vs. 0.5 mL) on Nile tilapia sperm quality after cryopreservation. Sperm was frozen according to conventional slow freezing procedure and diluted at ratio of 1:3 with ionic extender containing 350 mM glucose and 30 mM Tris containing 10% dimethylacetamide. Diluted semen was equilibrated at 4°C for 10 min and drawn into 0.25-mL or 0.5-mL plastic straws and sealed with polyvinyl alcohol. Samples were frozen 3 cm above of the liquid nitrogen surface and exposed to the liquid nitrogen vapor (≈−140°C) for 10 minutes After this, frozen sperm cells were kept into the liquid nitrogen container (−196°C). The frozen sperm in different volume of straws were thawed in a water bath at 30°C for 20 s (0.25-mL straws) or at 30°C for 30 s (0.5-mL straws), respectively. Fertilization was conducted using 1 ×105 spermatozoa/egg ratio with each straw type. The findings of the present study indicated that cryopreservation of sperm in glucose-Tris-based extender using 0.5-mL straws improved post-thaw progressive motility, duration of progressive motility, and fertilization results (P0.01). On the other hand, differences in term of post-thaw cell viability was not significant among the treatments (P0.01). In conclusion, our results suggest that Nile tilapia sperm can be successfully cryopreserved in Tris-based extenders supplemented with glucose containing 10% dimethylacetamide in 0.5-mL straws.
Author: Barrie G. M. Jamieson
Publisher: Cambridge University Press
ISBN: 0521413044
Category : Science
Languages : en
Pages : 335
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Book Description
In this 1991 book, Professor Jamieson masterfully brings together the literature on fish spermatozoa and voluminous work on the evolutionary history of fishes to provide a detailed synthesis of the two fields of fish spermatology and fish systematics. The author begins by considering invertebrate phyla related to the chordates, and goes through the lower chordates and early fishes to the line leading to amphibians and to highest teleosts. His treatment provides a review of fish systematics based on the classical evidence of gross morphology in a cladistic framework and a critical integration of this with information on the degree to which spermatozoa support of conflict with the various hypotheses of relationship. Additionally, Professor Jamieson is joined by Luke K. -P. Leung to give a review of the principles of biological cryopreservation and of the live preservation of fish gametes.
Author: C.-S. Lee
Publisher: Elsevier
ISBN: 0080929869
Category : Technology & Engineering
Languages : en
Pages : 328
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Book Description
The successful reproduction of cultured brood stock is essential to the sustainable aquaculture of aquatic organisms. This book describes recent advances in the field of finfish reproductive biotechnology. The chapters in this volume are written by eminent scientists who review the progress and assess the status of biotechnology research that is applicable to the reproduction of finfish species for aquaculture. A wide range of topics is included starting with broodstock technologies such as broodstock genetics, broodstock nutrition, environmental control of maturation and impacts of stress on broodstock, gametes and progeny. The volume includes technologies for induction of ovulation and spermiation using synthetic hypothalamic peptides. Gamete technologies which are described include cryopreservation, chromosome set manipulation, disease prevention and control for gametes and embryos and the development of transgenic fish with enhanced production characteristics. Genetic and endocrine technologies for the production of monosex male and female fish stocks are also described. The closing chapter summarizes the discussion of each topic at the workshop, provides recommendations to industry and describes priorities of research and development. Researchers as well as teaching faculty in the aquaculture field will find this volume of great value.
Author: Jorma Piironen
Publisher: Nordic Council of Ministers
ISBN: 9789291203215
Category :
Languages : en
Pages : 36
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Book Description
Author: Joseph G. Cloud
Publisher:
ISBN:
Category : Salmonidae
Languages : en
Pages : 54
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Book Description
Author: Francisco Marco-Jimenez
Publisher: BoD – Books on Demand
ISBN: 9535127799
Category : Science
Languages : en
Pages : 230
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Book Description
Since accidentally discovering the ability of glycerol on protecting cells from freezing damage, many researchers have been pursuing to develop cryopreservation methods of a very wide range of cells and some tissues, and these have found widespread applications in biology and medicine. From the point of view of living organisms, cryopreservation is a useful tool for ex situ conservation of genetic resources together with its contribution on conservation of their biodiversity. Cryopreservation in Eukaryotes includes totally 12 chapters, which have been written by the expert researchers in the field. The chapters are a comprehensive collection of the most frequently used methods for eukaryotes. With this book, every researcher will better understand the principles, background, and current status of cryopreservation in particular organisms.
