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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Small-angle x-ray scattering (SAXS) is experimencing a renaisance in the studies of macromolecular solutions, allowing one to study the structure of native particles and to rapidly analyze structural changes in response to variations in external conditions. Novel data analysis methods, significantly enhanced resolution, and reliability of structural models are provided by the technique. Emerging automation of the experiment, data processing, and data interpretation make SAXS a streamlined tool for large-scale structural studies in molecular biology. The method provides low-resolution macromolecular shapes ab initio and is readily combined with other structural and biochemical techniques in multidisciplinary studies to build rigid body models of complexes and to characterize oligomeric mixtures and flexible systems. The novel approaches also became useful for non-biological systems and processes, e.g., the formation of nanoparticles.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Small-angle x-ray scattering (SAXS) is experimencing a renaisance in the studies of macromolecular solutions, allowing one to study the structure of native particles and to rapidly analyze structural changes in response to variations in external conditions. Novel data analysis methods, significantly enhanced resolution, and reliability of structural models are provided by the technique. Emerging automation of the experiment, data processing, and data interpretation make SAXS a streamlined tool for large-scale structural studies in molecular biology. The method provides low-resolution macromolecular shapes ab initio and is readily combined with other structural and biochemical techniques in multidisciplinary studies to build rigid body models of complexes and to characterize oligomeric mixtures and flexible systems. The novel approaches also became useful for non-biological systems and processes, e.g., the formation of nanoparticles.
Author: Dmitri I. Svergun
Publisher: OUP Oxford
ISBN: 0191507032
Category : Science
Languages : en
Pages : 369
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Book Description
Small-angle scattering of X-rays (SAXS) and neutrons (SANS) is an established method for the structural characterization of biological objects in a broad size range from individual macromolecules (proteins, nucleic acids, lipids) to large macromolecular complexes. SAXS/SANS is complementary to the high resolution methods of X-ray crystallography and nuclear magnetic resonance, allowing for hybrid modeling and also accounting for available biophysical and biochemical data. Quantitative characterization of flexible macromolecular systems and mixtures has recently become possible. SAXS/SANS measurements can be easily performed in different conditions by adding ligands or binding partners, and by changing physical and/or chemical characteristics of the solvent to provide information on the structural responses. The technique provides kinetic information about processes like folding and assembly and also allows one to analyze macromolecular interactions. The major factors promoting the increasingly active use of SAXS/SANS are modern high brilliance X-ray and neutron sources, novel data analysis methods, and automation of the experiment, data processing and interpretation. In this book, following the presentation of the basics of scattering from isotropic macromolecular solutions, modern instrumentation, experimental practice and advanced analysis techniques are explained. Advantages of X-rays (rapid data collection, small sample volumes) and of neutrons (contrast variation by hydrogen/deuterium exchange) are specifically highlighted. Examples of applications of the technique to different macromolecular systems are considered with specific emphasis on the synergistic use of SAXS/SANS with other structural, biophysical and computational techniques.
Author:
Publisher: Academic Press
ISBN: 0323991807
Category : Science
Languages : en
Pages : 578
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Book Description
Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on Small Angle Scattering: Methods for Structural Investigation
Author: Michael Reich
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Eaton E. Lattman
Publisher: Oxford University Press
ISBN: 0191649899
Category : Science
Languages : en
Pages : 248
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Book Description
Small angle solution scattering (SAS) is increasingly being applied to biological problems. It is a complementary technique that, when applied in appropriate circumstances with carefully structured questions, can provide unique information not available from other techniques. While small angle solution scattering has been around for some time, a confluence of recent developments has dramatically enhanced its power. Intense third generation X-ray sources, low noise detectors, development of new algorithms and the computational power to take advantage of these have all matured, and use of free-electron x-ray laser sources is on the horizon. Whole new classes of experiments and analyses have been created as a result. These include the generation of molecular envelopes, the ability to do time-resolved studies, and the ability to account for structural changes using modelling based on the SAS data. The technical improvements have also reduced the amount of time and material needed to carry out an experiment. Beamtime at synchrotron sources is in demand, workshops on the subject are popular and researchers adopting the technique as part of their repertoire are growing. With these in mind, this book was written to guide structural biologists who may wish to adopt the technique, understand its strengths and weaknesses or just have a general interest in its potential.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 290
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Book Description
Small-Angle X-ray Scattering (SAXS) in solution is an established technique for the structural characterization of biological macromolecules. SAXS allows one to study proteins, nucleic acids and their complexes in near physiological environments. olecules. The improvements described in this work, mainly devoted to molecular modelling and data analysis, shall overcome many of the limitations encountered when SAXS is employed to assess flexibility. At a more general level, this dissertation aims at encouraging the use of a hybrid approach as standard modus operandi in the field of structural biology where detailed atomic observations - offered by high resolution techniques - are synergistically combined with the overall picture offered by SAXS in solution.
Author: Fotis Spyropoulos
Publisher: Royal Society of Chemistry
ISBN: 178801216X
Category : Science
Languages : en
Pages : 516
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Book Description
The most useful properties of food, i.e. the ones that are detected through look, touch and taste, are a manifestation of the food’s structure. Studies about how this structure develops or can be manipulated during food production and processing are a vital part of research in food science. This book provides the status of research on food structure and how it develops through the interplay between processing routes and formulation elements. It covers food structure development across a range of food settings and consider how this alters in order to design food with specific functionalities and performance. Food structure has to be considered across a range of length scales and the book includes a section focusing on analytical and theoretical approaches that can be taken to analyse/characterise food structure from the nano- to the macro-scale. The book concludes by outlining the main challenges arising within the field and the opportunities that these create in terms of establishing or growing future research activities. Edited and written by world class contributors, this book brings the literature up-to-date by detailing how the technology and applications have moved on over the past 10 years. It serves as a reference for researchers in food science and chemistry, food processing and food texture and structure.
Author:
Publisher: Elsevier
ISBN: 0323991793
Category : Science
Languages : en
Pages : 576
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Book Description
Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation
Author: Alicia Esther Ares
Publisher: BoD – Books on Demand
ISBN: 9535128876
Category : Technology & Engineering
Languages : en
Pages : 230
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Book Description
X-ray scattering techniques are a family of nondestructive analytical techniques. Using these techniques, scientists obtain information about the crystal structure and chemical and physical properties of materials. Nowadays, different techniques are based on observing the scattered intensity of an X-ray beam hitting a sample as a function of incident and scattered angle, polarization, and wavelength. This book is intended to give overviews of the relevant X-ray scattering techniques, particularly about inelastic X-ray scattering, elastic scattering, grazing-incidence small-angle X-ray scattering, small-angle X-ray scattering, and high-resolution X-ray diffraction, and, finally, applications of X-ray spectroscopy to study different biological systems.
Author: Xinyi Zhang
Publisher:
ISBN: 9789813227668
Category : Synchrotron radiation
Languages : en
Pages : 526
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Book Description
"The book contains the latest synchrotron-based techniques and research results. All contributors are specialists or leading scientists in their fields. The book includes new techniques and methods that will potentially get wider applications in various disciplines"--
