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Author: Dirk Saerens
Publisher: Humana Press
ISBN: 9781617799679
Category : Medical
Languages : en
Pages : 580
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Book Description
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Author: Dirk Saerens
Publisher: Humana Press
ISBN: 9781617799679
Category : Medical
Languages : en
Pages : 580
Get Book Here
Book Description
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Author: Muthupandian Saravanan
Publisher: Springer Nature
ISBN: 3030743306
Category : Medical
Languages : en
Pages : 362
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Book Description
Nanotechnology is an interdisciplinary research field that integrates chemistry, engineering, biology, and medicine. Nanomaterials offer tremendous opportunity as well as challenges for researchers. Of course, cancer is one of the world's most common health problems, responsible for many deaths. Exploring efficient anticancer drugs could revolutionize treatment options and help manage cancer mortality. Nanomedicine plays a significant role in developing alternative and more effective treatment strategies for cancer theranostics. This book mainly focuses on the emerging trends using nanomaterials and nanocomposites as alternative anticancer material’s. The book is divided into three main topic areas: how to overcome existing traditional approaches to combat cancer, applying multiple mechanisms to target the cancer cells, and how nanomaterials can be used as effective carriers. The contents highlight recent advances in interdisciplinary research on processing, morphology, structure, and properties of nanostructured materials and their applications to combat cancer.Cancer Nanotheranostics is comprehensive in that it discusses all aspects of cancer nanotechnology. Because of the vast amount of information, it was decided to split this material into two volumes. In the first volume of Cancer Nanotheranostics, we discuss the role of different nanomaterials for cancer therapy, including lipid-based nanomaterials, protein and peptide-based nanomaterials, polymer-based nanomaterials, metal-organic nanomaterials, porphyrin-based nanomaterials, metal-based nanomaterials, silica-based nanomaterials, exosome-based nanomaterials and nano-antibodies. In the second volume, we discuss the nano-based diagnosis of cancer, nano-oncology for clinical applications, nano-immunotherapy, nano-based photothermal cancer therapy, nano-erythrosomes for cancer drug delivery, regulatory perspectives of nanomaterials, limitations of cancer nanotheranostics, the safety of nano-biomaterials for cancer nanotheranostics, multifunctional nanomaterials for targeting cancer nanotheranostics, and the role of artificial intelligence in cancer nanotheranostics.
Author: Roland E. Kontermann
Publisher: Springer Science & Business Media
ISBN: 3642209106
Category : Medical
Languages : en
Pages : 381
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Book Description
The concept of using bispecific antibodies for cancer therapy by retargeting immune effector cells was developed several years ago. Initial clinical studies were rather disappointing mainly due to low efficacy, severe side effects and the immunogenicity of the bispecific antibodies. The progress in antibody engineering finally led to the generation of new classes of bispecific antibodies lacking these obstacles. In addition, new applications were established, such as pre-targeting strategies in radioimmunotherapy and dual targeting approaches in order to improve binding, selectivity and efficacy. In this book, the different ways of generating bispecific antibodies are described, with emphasis on recombinant formats. The various applications of bispecific antibodies, e.g. in cellular cancer immunotherapy, radioimmunotherapy and pretargeting strategies are covered, and emerging applications such as dual targeting strategies, which involve the simultaneous inhibition of two targets, are addressed.
Author: Kevin A. Henry
Publisher: Frontiers Media SA
ISBN: 2889454320
Category :
Languages : en
Pages : 338
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Book Description
Single-domain antibodies (sdAbs) represent the minimal antigen binding-competent form of the immunoglobulin domain and have unique properties and applications. SdAbs are naturally produced as the variable domains of the heavy chain-only antibodies of camelid ruminants and cartilaginous fishes, but can also be engineered synthetically from autonomous human or mouse VH or VL domains. The scope of this research topic and associated e-book covers current understanding and new developments in (i) the biology, immunology and immunogenetics of sdAbs in camelids and cartilaginous fishes, (ii) strategies for sdAb discovery, (iii) protein engineering approaches to increase the solubility, stability and antigen-binding affinity of sdAbs and (iv) specialized applications of sdAbs in areas such diagnostics, imaging and therapeutics.
Author: Margaret Ackerman
Publisher: Academic Press
ISBN: 0123948185
Category : Medical
Languages : en
Pages : 376
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Book Description
Antibody Fc is the first single text to synthesize the literature on the mechanisms underlying the dramatic variability of antibodies to influence the immune response. The book demonstrates the importance of the Fc domain, including protective mechanisms, effector cell types, genetic data, and variability in Fc domain function. This volume is a critical single-source reference for researchers in vaccine discovery, immunologists, microbiologists, oncologists and protein engineers as well as graduate students in immunology and vaccinology. Antibodies represent the correlate of protection for numerous vaccines and are the most rapidly growing class of drugs, with applications ranging from cancer and infectious disease to autoimmunity. Researchers have long understood the variable domain of antibodies, which are responsible for antigen recognition, and can provide protection by blocking the function of their target antigen. However, recent developments in our understanding of the protection mediated by antibodies have highlighted the critical nature of the antibody constant, or Fc domain, in the biological activity of antibodies. The Fc domain allows antibodies to link the adaptive and innate immune systems, providing specificity to a wide range of innate effector cells. In addition, they provide a feedback loop to regulate the character of the immune response via interactions with B cells and antigen-presenting cells. - Clarifies the different mechanisms of IgG activity at the level of the different model systems used, including human genetic, mouse, and in vitro - Covers the role of antibodies in cancer, infectious disease, and autoimmunity and in the setting of monoclonal antibody therapy as well as naturally raised antibodies - Color illustrations enhance explanations of the immune system
Author: Stefan Dübel
Publisher: John Wiley & Sons
ISBN: 3527329374
Category : Science
Languages : en
Pages : 2538
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Book Description
Dieses Nachschlagewerk zu therapeutischen Antikörpern sucht auch in der komplett überarbeiteten 2. Auflage seinesgleichen und bietet 30 % neue Inhalte zu Entwicklung, Herstellung und therapeutischen Anwendungen dieser Biomoleküle.
Author: Ulrich Rothbauer
Publisher: MDPI
ISBN: 3036503781
Category : Science
Languages : en
Pages : 300
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Book Description
Nanobodies have become outstanding tools for biomedical research, diagnostics and therapy. Recent advances in the identification and functionalization of target-specific nanobodies now make nanobody-based approaches broadly available to many researches in the field. This book provides a compilation of original research articles and comprehensive reviews covering important and up to date aspects of research on nanobodies and their applications for immunoassays, proteomics, protein crystallization and in vitro and in vivo imaging.
Author: J. Donald Capra
Publisher: Karger Publishers
ISBN: 9783805563567
Category : Medical
Languages : en
Pages : 218
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Book Description
The last decade has witnessed remarkable developments in antibody research and its therapeutic applications. With the methods of molecular biology it is now possible to manipulate the specificities and activities of antibody molecules to generate an almost limitless array of structures for both basic investigations and the clinical setting. The contributions to this volume cover all three domains of the antibody: the variable regions, the relatively neglected but crucial hinge, and the constant region. These studies provide critical structural and functional information about antibodies, while also pointing the way to the construction of molecules with enhanced or even novel properties. Bringing together major experts on antibody engineering, this book is highly recommended to faculty, postdoctoral fellows and graduate students in molecular biology, microbiology, immunology, cancer research and genetics.
Author: Carlos F. Barbas
Publisher: CSHL Press
ISBN: 0879697407
Category : Science
Languages : en
Pages : 724
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Book Description
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Author: Christian Schönbach
Publisher: Springer Science & Business Media
ISBN: 0387729682
Category : Medical
Languages : en
Pages : 216
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Book Description
In contrast to existing books on immunoinformatics, this volume presents a cross-section of immunoinformatics research. The contributions highlight the interdisciplinary nature of the field and how collaborative efforts among bioinformaticians and bench scientists result in innovative strategies for understanding the immune system. Immunoinformatics is ideal for scientists and students in immunology, bioinformatics, microbiology, and many other disciplines.
