Separation of Bovine Serum Albumin (BSA) Protein by Foam Fractionation Technique PDF Download
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Author: Avishek Mandal
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
Foam fractionation is an effective, low-cost, and environmentally friendly method for water treatment that is widely applied to the removal of hazardous materials, organic materials, and metal ions from the wastewater by using the surfactant as a collector. This type of process known as the adsorption bubble separation technique. It uses bubbles as a separation medium and concentrates the surfactant from its aqueous solution by the difference of adsorption properties of the surfactant on gas,Äìliquid interfaces. During the process of foam fractionation, it spared gas through the bottom of the column to create dispersed rising bubbles. They adsorbed the surfactant onto gas,Äìliquid interfaces of the rising bubbles. We discuss here separate Bovine Serum Albumin from aqueous solution with haemoglobin by foam fraction method. To investigate the effect of the following variables on the enrichment ratio of total protein, the separation process like concentration of feed, the effect of pH, and the Effect of gas flow rate.
Author: Avishek Mandal
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
Foam fractionation is an effective, low-cost, and environmentally friendly method for water treatment that is widely applied to the removal of hazardous materials, organic materials, and metal ions from the wastewater by using the surfactant as a collector. This type of process known as the adsorption bubble separation technique. It uses bubbles as a separation medium and concentrates the surfactant from its aqueous solution by the difference of adsorption properties of the surfactant on gas,Äìliquid interfaces. During the process of foam fractionation, it spared gas through the bottom of the column to create dispersed rising bubbles. They adsorbed the surfactant onto gas,Äìliquid interfaces of the rising bubbles. We discuss here separate Bovine Serum Albumin from aqueous solution with haemoglobin by foam fraction method. To investigate the effect of the following variables on the enrichment ratio of total protein, the separation process like concentration of feed, the effect of pH, and the Effect of gas flow rate.
Author: Muhammad Abubakar
Publisher: BoD – Books on Demand
ISBN: 1839695080
Category : Medical
Languages : en
Pages : 308
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Book Description
Bovine Science - Challenges and Advances presents up-to-date knowledge of bovine health, covering both introductory topics and more advanced concepts. Chapters cover such topics as new techniques in bovine science and development, health and risk factors and diagnosis of disease in bovines, and production and reproductive technologies and advancements.
Author: Robert W. Schnepf
Publisher:
ISBN:
Category :
Languages : en
Pages : 28
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Book Description
Author: Paul Stevenson
Publisher: CRC Press
ISBN: 1466558520
Category : Medical
Languages : en
Pages : 204
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Book Description
Foam fractionation is a separation process in which proteins and other amphipathic species adsorb to the surface of bubbles. The bubbles are then removed from the solution in the form of foam at the top of a column. Due to its cost-effectiveness, foam fractionation has the potential for rapid commercial growth, especially in biotechnology.To assist
Author: Mohd.Raflie Maslih
Publisher:
ISBN:
Category : Proteins
Languages : en
Pages : 53
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Book Description
The aim for this research is to produces protein separation tool by a new technique to separate protein molecule from protein solution. The technique used is the molecularly imprinted polymer, MIP. In this technique the target molecule that we want to separate will be the template and it mixed with selected monomers. Through polymerization process the template molecule will trapped between the polymer matrix that become solid and stable in term of heat and chemical. To make it has high selectivity on protein molecule the solid polymer is pounded to fine particles and through hydrolysis process the template is extracted and will leave complementary sequences that have exact configuration like the template shape. And further, two type of study were carried out, the first was analysis of particles structure in characterization of particles by using FT-IR spectrum and the second was study on the performances of this technique by conducting three experiments. The first experiment was the adsorption of BSA protein in MIP or synthesized polymer particles, the second experiment was the effect of protein concentration on separation and third experiment was to observe the effect of particles mass on separation. Polymerization and hydrolysis was successfully occur and it shown by the FT-IR spectrum. Experiment show that adsorption of protein was high for MIP particle that was 52.5 % while adsorption for NIP particle was 33.0 %. For the effect of concentration and mass of particle, it shows that the availability particle is the main factor that influence the amount of substrate separated.
Author: Bambang Haryono
Publisher:
ISBN:
Category : Foam
Languages : en
Pages :
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Book Description
Author: Veara Loha
Publisher:
ISBN:
Category : Foam
Languages : en
Pages : 602
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Book Description
Author: Shyam Dedaniya
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 0
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Book Description
"The goal of this investigation is to develop a reversed-phase high-performance liquid chromatographic method to separate ovalbumin (OVA) and bovine serum albumin (BSA) proteins and to quantify BSA protein in its raw material. This technique is considered one of the most promising analytical techniques for the quantification of peptides and intact proteins, including monoclonal antibodies (mAbs). Two stationary phases, C4 and C3 with 300 Å pore size columns, were tested in this investigation. The C4 column is in 250 × 4.6 mm dimension, while the C3 column is in 75 × 2.1 mm dimension. A 0.08 % TFA was added to the mobile phase as an ion-pairing reagent to control its acidity and column efficiency. Proteins standard solutions of BSA and OVA were prepared in 6 M urea as a denaturing agent to improve chromatographic efficiency. The optimum separation conditions were found under a flow rate 1.0 mL/min, injection volume 20 μL, column temperature 50°C, and a gradient profile: 0 to 10 min, % B: 25% to 80%, 10 to 20 min, % B: 80% to 100% with solvent B = 100% acetonitrile + 0.08% trifluoroacetic acid. Under these separation conditions, the percentage recovery of BSA protein from the stationary phase was found 100%, while only 90- 95% of OVA was recovered. A short alkyl chain column (30 x 4.6 mm) helped improve the recovery of OVA protein and reduced the separation run time of the method. Forced degradation studies were conducted under the optimum separation conditions to determine the stability of these two proteins under sodium hydroxide, hydrochloric acid, hydrogen peroxide, heat, and light. In addition, the developed method was validated to verify its specificity and robustness and confirm its high accuracy and precision. The validation results fulfilled the U.S. Food and Drug Administration guidelines (FDA).--
Author: Robert Lemlich
Publisher: Elsevier
ISBN: 0323154816
Category : Technology & Engineering
Languages : en
Pages : 349
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Book Description
Adsorptive Bubble Separation Techniques focuses on the mechanisms of the various adsorptive bubble separation methods. This book examines the various adsorptive bubble separation techniques, including ion flotation, foam fractionation, precipitate flotation, mineral flotation, bubble fractionation, and solvent sublation. Organized into 20 chapters, this book starts with an overview of the certain important properties of foam. This text then examines the results of several separations, as well as the results of additional studies into the mechanisms of the different techniques. Other chapters explain the studies of foam separation in the case of synthetic solutions, which provide a good knowledge of the extraction mechanisms of the radioactive cations, cesium, cerium, and strontium. This book discusses as well the experimental and theoretical work on foam separation done in Israel. The final chapter deals with the separation of surfactants and metallic ions at various places around the world. This book is a valuable resource for materials scientists, engineers, and chemists.
Author: Scott David Webb
Publisher:
ISBN:
Category :
Languages : en
Pages : 640
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Book Description
