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Author: Jun Liu
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Heterogeneity is the hallmark of cell biology. Robotic system and automated methods are required for characterization and manipulation of a large number of single cells for many important applications, such as characterizing cell-cell communication for screening drug effects, processing oocytes or embryos for cryopreservation, and analyzing sperm locomotion behavior for selection of a high-quality sperm for in vitro fertilization (IVF). However, current robotic characterization and manipulation systems have two major limitations in search-and-locating end-effector tips and determination of relative Z position between end-effector tips and single cells. This research has addressed these two limitations by developing an auto-locating end-effector tip method and two vision-based contact detection algorithms. By integrating the two new solutions, I have built two robotic system prototypes for microinjection of a large number of adherent cells and automatically processing oocytes or embryos for vitrification. The robotic microinjection system enabled automated gap junction measurement for repurposing antiarrhythmic drugs to identify optimal treatments for cardiac arrhythmias by assessing each drugâ s efficacy on rescuing/enhancing gap junctions. Robotic vitrification relieves human operators from tedious and difficult manual steps, eliminating operator errors and inconsistencies. It also offers unparalleled timing control based on real-time embryo volume monitoring. Automation enables the system to process multiple oocytes/embryos with high efficiency. In addition, automated methods were developed to quantitatively analyze sperm locomotion behaviors by tracking both sperm head and tail. Experimental results on automated sperm analysis revealed a significant correlation between sperm head velocity and tail beating amplitude. By combining automated sperm motility analysis with DNA assessment technique, this research offers a new solution for identifying single sperms of high quality for IVF applications. In the end, this research also reviewed future research directions in microsystems and nanoengineering for intracellular measurement and manipulations and discussed the promising research for the intracellular study.
Author: Jun Liu
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Heterogeneity is the hallmark of cell biology. Robotic system and automated methods are required for characterization and manipulation of a large number of single cells for many important applications, such as characterizing cell-cell communication for screening drug effects, processing oocytes or embryos for cryopreservation, and analyzing sperm locomotion behavior for selection of a high-quality sperm for in vitro fertilization (IVF). However, current robotic characterization and manipulation systems have two major limitations in search-and-locating end-effector tips and determination of relative Z position between end-effector tips and single cells. This research has addressed these two limitations by developing an auto-locating end-effector tip method and two vision-based contact detection algorithms. By integrating the two new solutions, I have built two robotic system prototypes for microinjection of a large number of adherent cells and automatically processing oocytes or embryos for vitrification. The robotic microinjection system enabled automated gap junction measurement for repurposing antiarrhythmic drugs to identify optimal treatments for cardiac arrhythmias by assessing each drugâ s efficacy on rescuing/enhancing gap junctions. Robotic vitrification relieves human operators from tedious and difficult manual steps, eliminating operator errors and inconsistencies. It also offers unparalleled timing control based on real-time embryo volume monitoring. Automation enables the system to process multiple oocytes/embryos with high efficiency. In addition, automated methods were developed to quantitatively analyze sperm locomotion behaviors by tracking both sperm head and tail. Experimental results on automated sperm analysis revealed a significant correlation between sperm head velocity and tail beating amplitude. By combining automated sperm motility analysis with DNA assessment technique, this research offers a new solution for identifying single sperms of high quality for IVF applications. In the end, this research also reviewed future research directions in microsystems and nanoengineering for intracellular measurement and manipulations and discussed the promising research for the intracellular study.
Author: Tuhin Subhra Santra
Publisher: MDPI
ISBN: 3036506284
Category : Science
Languages : en
Pages : 254
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Book Description
Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Author: Alice D. Domar
Publisher: Cambridge University Press
ISBN: 110879677X
Category : Medical
Languages : en
Pages : 189
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Book Description
An essential resource for reproduction professionals wishing to understand patient-centered advanced technologies now and in the future.
Author: Barry D. Bavister
Publisher: Springer Science & Business Media
ISBN: 1461393175
Category : Science
Languages : en
Pages : 359
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Book Description
This volume contains the Proceedings of the Serono Symposium on Pre implantation Embryo Development, held in Newton, Massachusetts, in 1991. The idea for the symposium grew out of the 1989 Serono Symposium on Fertilization in Mammals* at which preimplantation development was the predominant suggestion for a follow-up topic. This was indeed a timely subject in view of the recent resurgence of interest in this funda mental phase of embryogenesis and its relevance to basic research and applied fertility studies in humans, food-producing animals, and endangered species. The symposium brought together speakers from a broad range of disciplines in order to focus on key regulatory mechanisms in embryo development, using a wide variety of animal models, and on representative topics in human preimplantation embryogenesis. The culmination of preimplantation development is a blastocyst con taining the first differentiated embryonic tissues and capable of initiating and sustaining pregnancy. The central objective of the symposium was to throw light on the regulation of cellular and molecular events underlying blastocyst formation. It was particularly appropriate that the date of the symposium marked the 20th anniversary of the publication of the classic volume Biology of the Blastocyst, the proceedings of an international workshop held in 1970. This book, which summarized most of the information then available on this topic in mammals, was edited by the pioneer in blastocyst research, Dr. Richard B1andau, who was the guest speaker at the symposium.
Author: National Intelligence Council
Publisher: Cosimo Reports
ISBN: 9781646794973
Category :
Languages : en
Pages : 158
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Book Description
"The ongoing COVID-19 pandemic marks the most significant, singular global disruption since World War II, with health, economic, political, and security implications that will ripple for years to come." -Global Trends 2040 (2021) Global Trends 2040-A More Contested World (2021), released by the US National Intelligence Council, is the latest report in its series of reports starting in 1997 about megatrends and the world's future. This report, strongly influenced by the COVID-19 pandemic, paints a bleak picture of the future and describes a contested, fragmented and turbulent world. It specifically discusses the four main trends that will shape tomorrow's world: - Demographics-by 2040, 1.4 billion people will be added mostly in Africa and South Asia. - Economics-increased government debt and concentrated economic power will escalate problems for the poor and middleclass. - Climate-a hotter world will increase water, food, and health insecurity. - Technology-the emergence of new technologies could both solve and cause problems for human life. Students of trends, policymakers, entrepreneurs, academics, journalists and anyone eager for a glimpse into the next decades, will find this report, with colored graphs, essential reading.
Author: Patrick Quinn
Publisher: Cambridge University Press
ISBN: 1139917358
Category : Medical
Languages : en
Pages : 307
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Book Description
This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
Author: Carlos Simón
Publisher: Cambridge University Press
ISBN: 1107034477
Category : Medical
Languages : en
Pages : 199
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Book Description
Stem cell science has the potential to impact human reproductive medicine significantly - cutting edge technologies allow the production and regeneration of viable gametes from human stem cells offering potential to preciously infertile patients. Written by leading experts in the field Stem Cells in Reproductive Medicine brings together chapters on the genetics and epigenetics of both the male and female gametes as well as advice on the production and regeneration of gene cells in men and women, trophoblasts and endometrium from human embryonic and adult stem cells. Although focussing mainly on the practical elements of the use of stem cells in reproductive medicine, the book also contains a section on new developments in stem cell research. The book is essential reading for reproductive medicine clinicians, gynecologists and embryologists who want to keep abreast of practical developments in this rapidly developing field.
Author: Patrick Fénichel
Publisher: John Libbey Eurotext
ISBN: 9782742000944
Category : Medical
Languages : en
Pages : 518
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Book Description
This valuable reference work provides a synthesis of the latest knowledge about the acrosomatic reaction of human sperm. The acrosomatic reaction, which is a fundamental step in the process of fertilisation, is examined on both the fundamental and clinical levels. Three very recent aspects are also dealt with: what happens to the acrosome during intracytoplasmic microinjections, the usefulness of screening spermatozoa that have completed their acrosomatic reaction, and the development of contraception by a vaccine that targets one of the proteins of the acrosome. International Symposium, Collioure, France, September 1995.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309154006
Category : Science
Languages : en
Pages : 246
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Book Description
A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.
Author: Ashutosh Tiwari
Publisher: John Wiley & Sons
ISBN: 1118773683
Category : Technology & Engineering
Languages : en
Pages : 421
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Book Description
Offers a comprehensive and interdisciplinary view of cutting-edge research on advanced materials for healthcare technology and applications Advanced healthcare materials are attracting strong interest in fundamental as well as applied medical science and technology. This book summarizes the current state of knowledge in the field of advanced materials for functional therapeutics, point-of-care diagnostics, translational materials, and up-and-coming bioengineering devices. Advanced Healthcare Materials highlights the key features that enable the design of stimuli-responsive smart nanoparticles, novel biomaterials, and nano/micro devices for either diagnosis or therapy, or both, called theranostics. It also presents the latest advancements in healthcare materials and medical technology. The senior researchers from global knowledge centers have written topics including: State-of-the-art of biomaterials for human health Micro- and nanoparticles and their application in biosensors The role of immunoassays Stimuli-responsive smart nanoparticles Diagnosis and treatment of cancer Advanced materials for biomedical application and drug delivery Nanoparticles for diagnosis and/or treatment of Alzheimers disease Hierarchical modelling of elastic behavior of human dental tissue Biodegradable porous hydrogels Hydrogels in tissue engineering, drug delivery, and wound care Modified natural zeolites Supramolecular hydrogels based on cyclodextrin poly(pseudo)rotaxane Polyhydroxyalkanoate-based biomaterials Biomimetic molecularly imprinted polymers
