Reversed Phase Liquid Chromatography Using the Ionic Liquid Isopropylammonium Formate and Comparison of Indirect Spectrophotometric Methods for Phosphate PDF Download
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Author: Matthew P. Collins
Publisher:
ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 74
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Book Description
A new room temperature ionic liquid, isopropylammonium formate, IPAF, has been synthesized. Its application as a mobile phase replacement for traditional organic reversed phase-high performance liquid chromatography solvents is demonstrated. Solvent characteristics of IPAF, such as, polarity index, viscosity, and solvent strength are measured. Various van Deemter plots using different mixing approaches of the mobile phase and different columns are compared. The utility of IPAF has been further shown to be a suitable modifier solvent for the separation of cytochrome c from tryptophan. At 50°C, IPAF was able to maintain cytochrome c in its native form during the course of a chromatography separation, while a similar methanol modifier causes denaturation. Our long term goal is to develop a semi-preparative method to separate proteins in their native conformation. A comparison of atomic absorption and molecular spectrophotometry for the use in determining phosphate in an undergraduate teaching environment is also presented.
Author: Matthew P. Collins
Publisher:
ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 74
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Book Description
A new room temperature ionic liquid, isopropylammonium formate, IPAF, has been synthesized. Its application as a mobile phase replacement for traditional organic reversed phase-high performance liquid chromatography solvents is demonstrated. Solvent characteristics of IPAF, such as, polarity index, viscosity, and solvent strength are measured. Various van Deemter plots using different mixing approaches of the mobile phase and different columns are compared. The utility of IPAF has been further shown to be a suitable modifier solvent for the separation of cytochrome c from tryptophan. At 50°C, IPAF was able to maintain cytochrome c in its native form during the course of a chromatography separation, while a similar methanol modifier causes denaturation. Our long term goal is to develop a semi-preparative method to separate proteins in their native conformation. A comparison of atomic absorption and molecular spectrophotometry for the use in determining phosphate in an undergraduate teaching environment is also presented.
Author: Jeffrey Scott Kiel
Publisher:
ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 260
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Book Description
Author: Ante M. Krstulovic
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 316
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Book Description
A comprehensive problem-solving approach to reversed-phase high-performance liquid chromatography covering the theoretical aspects and practical information needed in diverse areas of research. Also reviews RPLC applications in the biomedical/biochemical field.
Author: M.C. García-Álvarez-Coque
Publisher: Elsevier Inc. Chapters
ISBN: 0128071311
Category : Science
Languages : en
Pages : 29
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Book Description
The addition of reagents to an RPLC mobile phase enables the separation of ionizable compounds, inorganic anions, and metal ions using conventional instrumentation, silica-based materials, and hydro-organic mixtures, thanks to a variety of secondary equilibria. This gives rise to several chromatographic modes, whose main features are outlined in this chapter. The effect of the mobile phase pH on the retention of ionizable compounds is described, together with the recommended experimental practice. The mechanism of adsorption of amphiphilic anions or cations on the stationary phase to attract analytes with opposite charge or suppress the silanol activity is discussed. Different reagents, such as alkylammonium salts, surfactants (below and above the critical micelle concentration), perfluorinated carboxylate anions, chaotropic ions, and ionic liquids, are considered. The potential of metal chelation for the determination of metal ions and organic compounds is also summarized.
Author: Csaba Horváth
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 344
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Book Description
Author: Steven A. Cohen
Publisher: John Wiley & Sons
ISBN: 0470276258
Category : Science
Languages : en
Pages : 490
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Book Description
Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.
Author: Gábor Szepesi
Publisher: Wiley-VCH
ISBN:
Category : Science
Languages : en
Pages : 376
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Book Description
Author: Yun Chu
Publisher:
ISBN:
Category :
Languages : en
Pages : 126
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Book Description
Author: P. Jandera
Publisher: Elsevier
ISBN: 0080858341
Category : Science
Languages : en
Pages : 531
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Book Description
Gradient Elution in Column Liquid Chromatography
Author: A. Gentili
Publisher: Elsevier Inc. Chapters
ISBN: 0128071230
Category : Science
Languages : en
Pages : 56
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Book Description
High-performance liquid chromatography (HPLC) has become the technique of choice to perform an accurate determination of water-soluble and fat-soluble vitamins and provitamin A carotenoids in foods, especially for routine work. An overview of main HPLC methods for the individual and simultaneous vitamin analysis in foods is here presented. All precautions indispensable for handling these analytes, easily susceptible to degradation, are described as well as problems connected with the sample preparation, chromatographic separation and detection. Difficulties related to the development of multivitamin methods are also assessed and the potentiality of the latest extraction and chromatographic detection techniques are highlighted.
