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Author: Ray Wu
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 948
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Book Description
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Author: Ray Wu
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 948
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Book Description
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Author:
Publisher: Academic Press
ISBN: 1483295974
Category : Science
Languages : en
Pages : 793
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Book Description
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. - Enzymes in DNA research - DNA isolation, hybridization, and cloning - DNA sequence analysis - cDNA cloning - Gene products - Identification of cloned genes and mapping of genes - Monitoring cloned gene expression - Cloning and transferring of genes into yeast cells - Cloning and transferring of genes into plant cells - Cloning and transferring of genes into animal cells - Site-directed mutagenesis - Protein engineering - Expression vectors
Author: Hyone-Myong Eun
Publisher: Elsevier
ISBN: 008053113X
Category : Science
Languages : en
Pages : 727
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Book Description
Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology - Presents information in an easily accessible format to serve as a quick reference source - Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Author: Ray Wu
Publisher: Academic Press
ISBN: 0323137741
Category : Science
Languages : en
Pages : 935
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Book Description
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.* Methods for: * DNA isolation and cloning* Synthesizing complementary DNA (cDNA)* Cleaving and manipulating DNA * Selecting useful reporter genes* Constructing vectors for cloning genes* Constructing expression vectors* Site-directed mutagenesis and gene disruption* Identifying and mapping genes* Transforming animal and plant cells* Sequencing DNA* Amplifying and manipulating DNA and PCR* Detecting DNA - protein interaction
Author: Thomas L. James
Publisher: Academic Press
ISBN:
Category : Science
Languages : en
Pages : 528
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Book Description
This volume and its companion, Volume 339, supplement Volumes 176, 177, 239, and 261. Chapters are written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules.
Author: James D. Watson
Publisher: Macmillan
ISBN: 9780716722823
Category : Medical
Languages : en
Pages : 644
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Book Description
An overview of recombitant DNA techniques and surveys advances in recombinant molecular genetics, experimental methods and their results.
Author: Siddra Ijaz
Publisher: Cambridge Scholars Publishing
ISBN: 1527538443
Category : Science
Languages : en
Pages : 156
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Book Description
Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Author: Bernard R. Glick
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 724
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Book Description
The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Judith W. Zyskind
Publisher: Elsevier
ISBN: 1483220974
Category : Science
Languages : en
Pages : 210
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Book Description
Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
