Quantifying the Behavioral Response to Optogenetic Stimulation of Mechanosensory Neurons in C. Elegans PDF Download
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Author: Miloš Nikolić
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Author: Miloš Nikolić
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Author: Mochi Liu
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
One of the fundamental problems in neuroscience is how behavior is generated from sensory input and internal neural states, such as the animal?s behavioral context. We present new methods and findings to address this by studying the model organism Caenorhabditis elegans. With a fully mapped connectome of 302 neurons, this nematode is a particularly good candidate to investigate the neural basis of behavior due to its rich history of scientific research and its optical transparency. First, we showcase an instrument that can record panneuronal calcium activity in the head of a freely moving worm at single neuron resolution. We find multiple neurons have correlated activity with behaviors such as forward, backward, and turning locomotion. We also developed a high-throughput method to measure sensorimotor transformations from soft touch stimulation to locomotory behavior. We use automated behavior segmentation and reverse correlation to reveal how mechanosensory stimuli influences behavioral transitions. Our results show that C. elegans make locomotory decisions based on both the temporal history of the stimulus and its own behavioral context in a predictable manner. Continuing our investigation of the soft touch circuit, we developed a more advanced apparatus that can probe worm behavioral response to excitatory and inhibitory optogenetic stimuli with sub-animal level spatial resolution. This instrument has the ability to target the heads and tails of many animals in parallel, and can tailor the stimuli based on real-time behavior information. Preliminary experiments demonstrate that it can evoke the same optogenetically driven touch response akin to mechanical activation of the touch neurons.
Author: Arzu Çelik
Publisher: Springer
ISBN: 3319573632
Category : Medical
Languages : en
Pages : 517
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Book Description
This book offers representative examples from fly and mouse models to illustrate the ongoing success of the synergistic, state-of-the-art strategy, focusing on the ways it enhances our understanding of sensory processing. The authors focus on sensory systems (vision, olfaction), which are particularly powerful models for probing the development, connectivity, and function of neural circuits, to answer this question: How do individual nerve cells functionally cooperate to guide behavioral responses? Two genetically tractable species, mice and flies, together significantly further our understanding of these processes. Current efforts focus on integrating knowledge gained from three interrelated fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types (“connectomics”) using high-resolution three-dimensional circuit anatomy, and (3) causal testing of how iden tified circuit elements contribute to visual perception and behavior.
Author: Ellen Covey
Publisher: Oxford University Press
ISBN: 0199342547
Category : Medical
Languages : en
Pages : 241
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Book Description
Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.
Author: Sidney Cambridge
Publisher: Humana
ISBN: 9781493954353
Category : Science
Languages : en
Pages : 0
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Book Description
Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
Author: P. P. G. Bateson
Publisher: Cambridge University Press
ISBN: 9780521212878
Category : Science
Languages : en
Pages : 564
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Book Description
First published in 1976, this volume is a collection of essays by some of the most prominent and active ethologists. It is organized into four sections: motivation and perception, function and evolution, development, and human social relationships. The first three sections reflect the four questions which are basic to ethology: what were the immediate causes of a behaviour pattern; what is its biological function; how did it evolve; and how did it develop in the individual? The last section involves questions of all four types. The sections are introduced and linked by editorials and the book concludes with an important statement on asking the right questions. The essays are forward looking and identify areas of importance for the study of behaviour. The volume is a source of formative ideas for students, their teachers and research workers in a wide variety of disciplines in the biological psychological and social sciences.
Author: Hiromu Yawo
Publisher: Springer Nature
ISBN: 9811587639
Category : Medical
Languages : en
Pages : 648
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Book Description
This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular functions can thus be manipulated or probed by light. The new edition documents fully the extensive progress since publication of the first edition to provide an up-to-date overview of the physical, chemical, and biological properties of light-sensing proteins and their application in biological systems, particularly in neuroscience but also in medicine and the optical sciences. Underlying principles are explained and detailed information provided on a wide range of optogenetic tools for the observation and control of cellular signaling and physiology, gene targeting technologies, and optical methods for biological applications. In presenting the current status of optogenetics and emerging directions, this milestone publication will be a “must read” for all involved in research in any way related to optogenetics.
Author: Grigoriĭ Nikolaevich Orlovskiĭ
Publisher: Oxford Neuroscience
ISBN: 9780198524052
Category : Medical
Languages : en
Pages : 322
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Book Description
What does the swimming leech have to do with the running human? The ability to move actively in space is essential to members of the animal kingdom, and the evolution of the nervous system relates to a large extent to the evolution of locomotion. The extreme importance of locomotion hasstimulated many studies of the neural mechanisms underlying locomotion across a range of species. For the first time, a group of three leading neurobiologists have undertaken a comparative study of these mechanisms. Neuronal Control of Locomotion: From Mollusc to Man describes how the brains invery diverse and evolutionarily removed species control the animal's locomotion. In doing so, the authors reveal unifying principles of brain function, making it essential reading for students and researchers in neurobiology generally, and motor control in particular. "In my opinion, the authorshave produced a masterful and highly readable exposition on the neural control of locomotion. It is timely and relevant to avant- garde neuroscience. It will have a major impact on the field, and is sure to be referenced well into the second half of the next century." Douglas Stuart, Universityof Arizona College of Medicine
Author: Jordi Llorens
Publisher: Springer Nature
ISBN: 1071616374
Category : Medical
Languages : en
Pages : 488
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Book Description
This volume explores the latest methods that seek to address the vital questions being asked in neurotoxicology research. The chapters in this book cover a variety of available methods from the molecular level to the organism level, and both in vitro and in vivo approaches, including alternative model organisms. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Experimental Neurotoxicology Methods is a valuable resource for both young and experienced researchers who are looking for guidance to implement these methods in their laboratories or for understanding the data generated through these techniques.
Author: Scott L. Hooper
Publisher: John Wiley & Sons
ISBN: 1118873408
Category : Medical
Languages : en
Pages : 510
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Book Description
A multi-disciplinary look at the current state of knowledge regarding motor control and movement—from molecular biology to robotics The last two decades have seen a dramatic increase in the number of sophisticated tools and methodologies for exploring motor control and movement. Multi-unit recordings, molecular neurogenetics, computer simulation, and new scientific approaches for studying how muscles and body anatomy transform motor neuron activity into movement have helped revolutionize the field. Neurobiology of Motor Control brings together contributions from an interdisciplinary group of experts to provide a review of the current state of knowledge about the initiation and execution of movement, as well as the latest methods and tools for investigating them. The book ranges from the findings of basic scientists studying model organisms such as mollusks and Drosophila, to biomedical researchers investigating vertebrate motor production to neuroengineers working to develop robotic and smart prostheses technologies. Following foundational chapters on current molecular biological techniques, neuronal ensemble recording, and computer simulation, it explores a broad range of related topics, including the evolution of motor systems, directed targeted movements, plasticity and learning, and robotics. Explores motor control and movement in a wide variety of organisms, from simple invertebrates to human beings Offers concise summaries of motor control systems across a variety of animals and movement types Explores an array of tools and methodologies, including electrophysiological techniques, neurogenic and molecular techniques, large ensemble recordings, and computational methods Considers unresolved questions and how current scientific advances may be used to solve them going forward Written specifically to encourage interdisciplinary understanding and collaboration, and offering the most wide-ranging, timely, and comprehensive look at the science of motor control and movement currently available, Neurobiology of Motor Control is a must-read for all who study movement production and the neurobiological basis of movement—from molecular biologists to roboticists.
