Principles and Practices of DNA Analysis

Principles and Practices of DNA Analysis PDF Author: Hirak Ranjan Dash
Publisher:
ISBN: 9781071602744
Category : DNA fingerprinting
Languages : en
Pages : 344

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Book Description

Principles and Practices of DNA Analysis

Principles and Practices of DNA Analysis PDF Author: Hirak Ranjan Dash
Publisher:
ISBN: 9781071602744
Category : DNA fingerprinting
Languages : en
Pages : 344

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Book Description


Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing

Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing PDF Author: Hirak Ranjan Dash
Publisher: Humana
ISBN: 9781071602768
Category : Medical
Languages : en
Pages : 344

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Book Description
The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.

Forensic DNA Typing: Principles, Applications and Advancements

Forensic DNA Typing: Principles, Applications and Advancements PDF Author: Pankaj Shrivastava
Publisher: Springer Nature
ISBN: 9811566550
Category : Science
Languages : en
Pages : 673

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Book Description
The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

Forensic DNA Analysis

Forensic DNA Analysis PDF Author: J. Thomas McClintock
Publisher: CRC Press
ISBN: 1040084222
Category : Law
Languages : en
Pages : 165

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Book Description
In its short but active history, the use of DNA typing has revolutionized criminal investigations. It is almost inconceivable to bring a case to trial without positive identification through what is now our most accurate means. Proficiency with the methodology, principles, and interpretation of DNA evidence is crucial for today‘s criminalist.

Fundamentals of Forensic DNA Typing

Fundamentals of Forensic DNA Typing PDF Author: John M. Butler
Publisher: Academic Press
ISBN: 0080961762
Category : Law
Languages : en
Pages : 519

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Book Description
Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980's, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. - Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect - Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout - Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key

Techniques of Crime Scene Investigation

Techniques of Crime Scene Investigation PDF Author: Barry A. J. Fisher
Publisher: CRC Press
ISBN: 1498758142
Category : Law
Languages : en
Pages : 369

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Book Description
"Techniques of Crime Scene Investigation is a staple for any forensic science library and is routinely referenced by professional organizations as a study guide for certifications. It is professionally written and provides updated theoretical and practical applications using real casework. This text is a must-have for any CSI Unit or course teaching Crime Scene Investigation." – Kevin Parmelee, PhD, Detective (ret.), Somerset County, NJ Prosecutor’s Office Since the first English-language edition of Techniques of Crime Scene Investigation was published in 1964, the book has continued to be a seminal work in the field of forensic science, serving as a foundational textbook and reference title for professionals. This Ninth Edition includes several new chapters and has been fully updated and organized to present the effective use of science and technology in support of justice. New coverage to this edition addresses the debunking of a few forensic science disciplines, long thought to have been based on sound science. The book provides students, crime scene investigators, forensic scientists, and attorneys the proper ways to examine crime scenes and collect a wide variety of physical evidence that may be encountered. While it is not possible to cover every imaginable situation, this book is a comprehensive guide that details and promotes best practices and recommendations. In today’s challenging environment, it is essential that law enforcement personnel thoroughly understand and meticulously comply with the forensic evidence procedures that apply to their function in the investigation process. Criminal investigations remain as complex as ever and require professionals from many disciplines to work cooperatively toward the fair and impartial delivery of justice. Practitioners and students alike need to be aware of the increased scrutiny that they will face in the judicial system. Judges are taking a more involved role than ever before as far as the evidence and testimony that they allow into their courtrooms. No longer will substandard forensic science or crime scene investigation be acceptable. Key features: Newly reorganized contents—including 4 brand new chapters—reflects a more logical flow of crime scene processes and procedures Provides an overview of the crime scene investigation process and procedures, from the first officer on the scene through the adjudication of the case Includes several new cases, photos, and updates in technological advances in both digital evidence and DNA in particular Science and technology applied to CSI solves crimes and saves lives. Investigators, prosecutors, and defense attorneys must be able to use forensic tools and resources to their fullest potential and Techniques of Crime Scene Investigation serves as an invaluable resource to further this cause.

A Handbook on Techniques of Molecular Biology

A Handbook on Techniques of Molecular Biology PDF Author: Dr. Manti Debnath
Publisher: OrangeBooks Publication
ISBN:
Category : Science
Languages : en
Pages : 182

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Book Description
This handbook is a comprehensive guide for molecular biologists and researchers, offering detailed protocols and methodologies in molecular genetics and genomics. It covers key techniques such as DNA and RNA extraction, PCR, cDNA synthesis, and expression cloning. Advanced analytical tools like electrophoresis, blotting, and ELISA are discussed, along with modern technologies such as microarray, next-generation sequencing (NGS), and transcriptomics. The book also delves into genetic markers and polymorphic analyses using SSRs, RAPD, RFLP, AFLP, and innovative approaches like SAMPL. Designed as a practical resource, it is invaluable for gene expression studies, protein detection, and genetic analysis.

The Evaluation of Forensic DNA Evidence

The Evaluation of Forensic DNA Evidence PDF Author: National Research Council
Publisher: National Academies Press
ISBN: 0309121949
Category : Science
Languages : en
Pages : 271

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Book Description
In 1992 the National Research Council issued DNA Technology in Forensic Science, a book that documented the state of the art in this emerging field. Recently, this volume was brought to worldwide attention in the murder trial of celebrity O. J. Simpson. The Evaluation of Forensic DNA Evidence reports on developments in population genetics and statistics since the original volume was published. The committee comments on statements in the original book that proved controversial or that have been misapplied in the courts. This volume offers recommendations for handling DNA samples, performing calculations, and other aspects of using DNA as a forensic toolâ€"modifying some recommendations presented in the 1992 volume. The update addresses two major areas: Determination of DNA profiles. The committee considers how laboratory errors (particularly false matches) can arise, how errors might be reduced, and how to take into account the fact that the error rate can never be reduced to zero. Interpretation of a finding that the DNA profile of a suspect or victim matches the evidence DNA. The committee addresses controversies in population genetics, exploring the problems that arise from the mixture of groups and subgroups in the American population and how this substructure can be accounted for in calculating frequencies. This volume examines statistical issues in interpreting frequencies as probabilities, including adjustments when a suspect is found through a database search. The committee includes a detailed discussion of what its recommendations would mean in the courtroom, with numerous case citations. By resolving several remaining issues in the evaluation of this increasingly important area of forensic evidence, this technical update will be important to forensic scientists and population geneticistsâ€"and helpful to attorneys, judges, and others who need to understand DNA and the law. Anyone working in laboratories and in the courts or anyone studying this issue should own this book.

Strengthening Forensic Science in the United States

Strengthening Forensic Science in the United States PDF Author: National Research Council
Publisher: National Academies Press
ISBN: 0309142393
Category : Law
Languages : en
Pages : 348

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Book Description
Scores of talented and dedicated people serve the forensic science community, performing vitally important work. However, they are often constrained by lack of adequate resources, sound policies, and national support. It is clear that change and advancements, both systematic and scientific, are needed in a number of forensic science disciplines to ensure the reliability of work, establish enforceable standards, and promote best practices with consistent application. Strengthening Forensic Science in the United States: A Path Forward provides a detailed plan for addressing these needs and suggests the creation of a new government entity, the National Institute of Forensic Science, to establish and enforce standards within the forensic science community. The benefits of improving and regulating the forensic science disciplines are clear: assisting law enforcement officials, enhancing homeland security, and reducing the risk of wrongful conviction and exoneration. Strengthening Forensic Science in the United States gives a full account of what is needed to advance the forensic science disciplines, including upgrading of systems and organizational structures, better training, widespread adoption of uniform and enforceable best practices, and mandatory certification and accreditation programs. While this book provides an essential call-to-action for congress and policy makers, it also serves as a vital tool for law enforcement agencies, criminal prosecutors and attorneys, and forensic science educators.

Advancements in Forensic DNA Analysis

Advancements in Forensic DNA Analysis PDF Author: Hirak Ranjan Dash
Publisher: Springer Nature
ISBN: 9819961955
Category :
Languages : en
Pages : 161

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Book Description