Nonradioactive Labeling and Detection of Biomolecules PDF Download
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Author: Christoph Kessler
Publisher: Springer
ISBN: 3662001446
Category : Science
Languages : en
Pages : 449
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Book Description
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author: Christoph Kessler
Publisher: Springer
ISBN: 3662001446
Category : Science
Languages : en
Pages : 449
Get Book Here
Book Description
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author: Christoph Kessler
Publisher: Springer Science & Business Media
ISBN: 9783540646013
Category : Science
Languages : en
Pages : 1170
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Book Description
Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author: Christoph Kessler
Publisher: Springer Verlag
ISBN: 9780387554822
Category : Science
Languages : en
Pages : 436
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Book Description
Author: A. J. Garman
Publisher: Elsevier
ISBN: 0080551092
Category : Science
Languages : en
Pages : 155
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Book Description
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. Aimed at researchers without specific expertise in chemistry, the book includes: A review of the main signal systems and labels available, indicating their strengths and weaknesses Discussion of the most useful strategies for labelling the various biomolecules 32 protocols covering common labelling needs Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications Sources of information including references, data and suppliers
Author: Christoph Kessler
Publisher:
ISBN: 9783642572074
Category :
Languages : en
Pages : 768
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Book Description
Author: Gary C. Howard
Publisher: McGraw-Hill/Appleton & Lange
ISBN: 9780444016614
Category : Science
Languages : en
Pages : 342
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Book Description
Protein methods. Biotin labeling methods for proteins and carbohydrates. Direct conjugation of enzymes to proteins. Labeling proteins with fluorochromes. Comparing fluorescent organic dyes for biomolecular labeling. Chemiluminescent detection using alkaline phosphatase-the chemistry. Detection of proteins on filters by enzymatic methods. Enzyme-linked immunosorbent assays. Automation of protein and immunochemistry methods. DNA methods. Biotin labeling methods for DNA. Direct attachment of enzymes to DNA probes. Detection of nucleic acids on filters by enzymatic methods. Polymerase chain reaction and its impact on DNA detection limits. Automated procedures for DNA methods. Tissue and cell methods. Enzyme-based methods in tissue sections. In situ hybridization. Chromosome mapping by fluorescent in situ hybridization. Colloidal gold localization of in situ hybrids. Immunofluorescence methods for microscopic analysis. Flow cytometry methods.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
"Biological Techniques" is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels. This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.; This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. It is aimed at researchers without specific expertise in chemistry, the book includes: a review of the main signal systems and labels available, indicating their strengths and weaknesses. It includes a discussion of the most useful strategies for labelling the various biomolecules. There are 32 protocols covering common labelling needs. There are descriptions of the factors governing protocol design, enabling protocols to be modified for different applications. It contains sources of information including references, data and suppliers.
Author: Christoph Kessler
Publisher: Springer Science & Business Media
ISBN: 3642572065
Category : Science
Languages : en
Pages : 759
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Book Description
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.
Author: Boehringer Mannheim GmbH Biochemica
Publisher:
ISBN:
Category :
Languages : en
Pages : 62
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Book Description
Author: Larry J. Kricka
Publisher: Elsevier
ISBN: 0080537669
Category : Science
Languages : en
Pages : 539
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Book Description
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules Gives protocols for nonisotopic DNA sequencing - new in this edition Gives extensive, practical information Presents background information for each method Provides expert accounts from the inventor or developer of each method Contains seven entirely new chapters Covers all major types of nonisotopic procedures for labeling and detection
