Multi-Parametric Live Cell Microscopy of 3D Tissue Models PDF Download
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Author: Ruslan I. Dmitriev
Publisher: Springer
ISBN: 3319673580
Category : Science
Languages : en
Pages : 172
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Book Description
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Author: Ruslan I. Dmitriev
Publisher: Springer
ISBN: 3319673580
Category : Science
Languages : en
Pages : 172
Get Book Here
Book Description
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Author: Dmitri B Papkovsky
Publisher: Royal Society of Chemistry
ISBN: 1788014553
Category : Science
Languages : en
Pages : 384
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Book Description
Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.
Author: Paloma Ordóñez-Morán
Publisher: Springer Nature
ISBN: 1071630768
Category : Science
Languages : en
Pages : 273
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Book Description
This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Intestinal Differentiated Cells: Methods and Protocols aims to be comprehensive guide for researchers.
Author: Nirmal Mazumder
Publisher: Springer Nature
ISBN: 9811913528
Category : Medical
Languages : en
Pages : 265
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Book Description
The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.
Author: Kaoru Yamanouchi
Publisher: Springer
ISBN: 303005974X
Category : Science
Languages : en
Pages : 521
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Book Description
This second volume of “Progress in Photon Science – Recent Advances” presents the latest achievements made by world-leading researchers in Russia and Japan. Thanks to recent advances in light source technologies; detection techniques for photons, electrons, and charged particles; and imaging technologies, the frontiers of photon science are now being expanding rapidly. Readers will be introduced to the latest research efforts in this rapidly growing research field through topics covering bioimaging and biological photochemistry, atomic and molecular phenomena in laser fields, laser–plasma interaction, advanced spectroscopy, electron scattering in laser fields, photochemistry on novel materials, solid-state spectroscopy, photoexcitation dynamics of nanostructures and clusters, and light propagation.
Author:
Publisher: Frontiers Media SA
ISBN: 2889712524
Category : Science
Languages : en
Pages : 156
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Book Description
Author: Wolfgang Becker
Publisher: Springer
ISBN: 3319149296
Category : Science
Languages : en
Pages : 642
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Book Description
This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.
Author: Susie Nilsson
Publisher: Academic Press
ISBN: 0323910920
Category : Science
Languages : en
Pages : 218
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Book Description
Recapitulating The Stem Cell Niche Ex Vivo, Volume Six in the Advances in Stem Cells and their Niches series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Recapitulating the bone marrow stem cell niche ex vivo, The generation of the liver ex vivo, Recapitulating the thymic stem cell niche ex vivo, Recapitulating the intestinal epithelium stem cell niche ex vivo, Recapitulating the lung stem cell niche in vitro, Recapitulating mammary tissue in vitro, and Recapitulating muscle in vitro. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Advances in Stem Cells and their Niches series - Includes the latest information on Recapitulating the stem cell niche ex vivo
Author: Giulia Adriani
Publisher: Frontiers Media SA
ISBN: 2832537030
Category : Medical
Languages : en
Pages : 171
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Book Description
Author: Xiangsheng Zuo
Publisher: Frontiers Media SA
ISBN: 2832546285
Category : Science
Languages : en
Pages : 170
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Book Description
Tumor microenvironment (TME) refers to the area with non-malignant cells, signaling molecules and blood vessels that surrounds and feeds tumor cells. A tumor can change its microenvironment, and TME is also known to profoundly impact the occurrence, growth, metastasis and therapeutic response and resistance of tumors. The special paracrine characteristics, microvascular system, extracellular matrix components and stromal cells in TME promote local tumor immune suppression and could alter the penetration and distribution of drugs into tumor tissues. Accumulating evidence demonstrates that currently available tumor therapies (i.e., chemotherapy, radiotherapy, anti-vascular therapy, small molecule targeted therapy, immune checkpoint inhibitors) also in turn greatly affect the structure, function, and physical and chemical properties of TME, leading to TME remodeling. However, whether and how interaction of tumor cells and TME modeling can be dissected and targeted to further understand the underlying molecular mechanisms and establish effective new tumor therapies remains elusive.
