Mining the Aegilops Tauschii Gene Pool

Mining the Aegilops Tauschii Gene Pool PDF Author: Bhanu Kalia
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description
Leaf rust, caused by fungus Puccinia triticina, is an important foliar disease of wheat worldwide. Breeding for race-nonspecific resistant cultivars is the best strategy to combat this disease. Aegilops tauschii, D genome donor of hexaploid wheat, has provided resistance to several pests and pathogens of wheat. To identify potentially new adult plant resistance (APR) genes, 371 geographically diverse Ae. tauschii accessions were evaluated in field with leaf rust (LR) composite culture of predominant races. Accessions from Afghanistan only displayed APR whereas both seedling resistance and APR were common in the Caspian Sea region. Seventeen accessions with high APR were selected for production of synthetic hexaploid wheat (SHW), using 'TetraPrelude' and/or 'TetraThatcher' as tetraploid parents. Six SHWs were produced and evaluated for APR to LR and resistance to tan spot at seedling stage. Genetic analysis and mapping of APR introgressed from accession TA2474 was investigated in recombinant inbred lines (RIL) population derived from cross between SHW, TA4161-L3 and spring wheat cultivar, 'WL711'. Genotyping-by-sequencing approach was used to genotype the RILs. Maximum disease severity (MDS) for LR was significantly correlated among all experiments and APR to LR was highly heritable trait in this population. Nine genomic regions significantly associated with APR to LR were QLr.ksu-1AL, QLr.ksu-1BS, QLr.ksu-1BL. 1, QLr.ksu-1BL. 2, QLr.ksu-2DS, QLr.ksu-2DL, QLr.ksu-5AL, QLr.ksu-5DL and QLr.ksu-6BL. Association of QLr.ksu-1BL. 1 with marker Xwmc44 indicated this locus could be slow-rusting APR gene, Lr46/Yr29. QTLs detected on 2DS, 2DL and 5DL were contributed by TA4161-L3 and are novel, along with QLr.ksu-5AL. Tan spot, caused by necrotrophic fungus, Pyrenophora tritici-repentis, has recently emerged as a damaging disease of wheat worldwide. To identify QTLs associated with resistance to Race 1 of P. tritici-repentis, F[subscript]2:3 population derived from cross between SHW, TA4161-L1 and winter wheat cultivar, 'TAM105' was used. Two major effect QTLs, QTs.ksu-1AS. 1 and QTs.ksu-7AS were significantly associated with tan spot resistance and contributed by TA4161-L1. QTs.ksu-7AS is a novel QTL and explained 17% of the phenotypic variation. Novel QTLs for APR to LR and tan spot identified in SHWs add new variation for broadening the gene pool of wheat and providing resources for breeding of durable resistant cultivars.

Mining the Aegilops Tauschii Gene Pool

Mining the Aegilops Tauschii Gene Pool PDF Author: Bhanu Kalia
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description
Leaf rust, caused by fungus Puccinia triticina, is an important foliar disease of wheat worldwide. Breeding for race-nonspecific resistant cultivars is the best strategy to combat this disease. Aegilops tauschii, D genome donor of hexaploid wheat, has provided resistance to several pests and pathogens of wheat. To identify potentially new adult plant resistance (APR) genes, 371 geographically diverse Ae. tauschii accessions were evaluated in field with leaf rust (LR) composite culture of predominant races. Accessions from Afghanistan only displayed APR whereas both seedling resistance and APR were common in the Caspian Sea region. Seventeen accessions with high APR were selected for production of synthetic hexaploid wheat (SHW), using 'TetraPrelude' and/or 'TetraThatcher' as tetraploid parents. Six SHWs were produced and evaluated for APR to LR and resistance to tan spot at seedling stage. Genetic analysis and mapping of APR introgressed from accession TA2474 was investigated in recombinant inbred lines (RIL) population derived from cross between SHW, TA4161-L3 and spring wheat cultivar, 'WL711'. Genotyping-by-sequencing approach was used to genotype the RILs. Maximum disease severity (MDS) for LR was significantly correlated among all experiments and APR to LR was highly heritable trait in this population. Nine genomic regions significantly associated with APR to LR were QLr.ksu-1AL, QLr.ksu-1BS, QLr.ksu-1BL. 1, QLr.ksu-1BL. 2, QLr.ksu-2DS, QLr.ksu-2DL, QLr.ksu-5AL, QLr.ksu-5DL and QLr.ksu-6BL. Association of QLr.ksu-1BL. 1 with marker Xwmc44 indicated this locus could be slow-rusting APR gene, Lr46/Yr29. QTLs detected on 2DS, 2DL and 5DL were contributed by TA4161-L3 and are novel, along with QLr.ksu-5AL. Tan spot, caused by necrotrophic fungus, Pyrenophora tritici-repentis, has recently emerged as a damaging disease of wheat worldwide. To identify QTLs associated with resistance to Race 1 of P. tritici-repentis, F[subscript]2:3 population derived from cross between SHW, TA4161-L1 and winter wheat cultivar, 'TAM105' was used. Two major effect QTLs, QTs.ksu-1AS. 1 and QTs.ksu-7AS were significantly associated with tan spot resistance and contributed by TA4161-L1. QTs.ksu-7AS is a novel QTL and explained 17% of the phenotypic variation. Novel QTLs for APR to LR and tan spot identified in SHWs add new variation for broadening the gene pool of wheat and providing resources for breeding of durable resistant cultivars.

Wild Crop Relatives: Genomic and Breeding Resources

Wild Crop Relatives: Genomic and Breeding Resources PDF Author: Chittaranjan Kole
Publisher: Springer Science & Business Media
ISBN: 3642143873
Category : Science
Languages : en
Pages : 341

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Book Description
Wild crop relatives are now playing a significant part in the elucidation and improvement of the genomes of their cultivated counterparts. This work includes comprehensive examinations of the status, origin, distribution, morphology, cytology, genetic diversity and available genetic and genomic resources of numerous wild crop relatives, as well as of their evolution and phylogenetic relationship. Further topics include their role as model plants, genetic erosion and conservation efforts, and their domestication for the purposes of bioenergy, phytomedicines, nutraceuticals and phytoremediation. Wild Crop Relatives: Genomic and Breeding Resources comprises 10 volumes on Cereals, Millets and Grasses, Oilseeds, Legume Crops and Forages, Vegetables, Temperate Fruits, Tropical and Subtropical Fruits, Industrial Crops, Plantation and Ornamental Crops, and Forest Trees. It contains 125 chapters written by nearly 400 well-known authors from about 40 countries.

Allele Mining for Genomic Designing of Oilseed Crops

Allele Mining for Genomic Designing of Oilseed Crops PDF Author: Chittaranjan Kole
Publisher: CRC Press
ISBN: 1040103367
Category : Science
Languages : en
Pages : 315

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Book Description
This book deliberates on the concept, strategies, tools, and techniques of allele mining in oilseed crops and its application potential in genome elucidation and improvement, including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and studies of gene expression and gene prediction. Available gene pools in global germplasm collections, specifically consisting of wild allied species and local landraces for almost all major crops, have facilitated allele mining. The development of advanced genomic techniques, including PCR-based allele priming and Eco-TILLING-based allele mining, is now widely used for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs and with genome plasticity to adapt to climate change scenarios. All these concepts and strategies, along with precise success stories, are presented in the chapters dedicated to the major oilseed crops. 1. This is the first book on the novel strategy of allele mining in oilseed crops for precise breeding. 2. This book presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources. 3. This book depicts case studies of PCR-based allele priming and Eco-TILLING based allele mining. 4. This book elaborates on gene discovery and gene prediction in major oilseed crops. This book will be useful to students and faculties in various plant science disciplines, including genetics, genomics, molecular breeding, agronomy, and bioinformatics; scientists in seed industries; and policymakers and funding agencies interested in crop improvement.

Applications of Fast Breeding Technologies in Crop Improvement and Functional Genomics Study

Applications of Fast Breeding Technologies in Crop Improvement and Functional Genomics Study PDF Author: Xingguo Ye
Publisher: Frontiers Media SA
ISBN: 2832552404
Category : Science
Languages : en
Pages : 234

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Book Description
During the latest ten years, fast breeding technologies have been effectively applied in crop trait modification, gene mapping, and functional genomics study, which include haploid induction based on inducer lines, genome editing mediated by CRISPR/Cas9, and molecular selection based on special markers. By using CRISPR/Cas9, many crop traits such as disease resistance, good quality, early maturity, high grain weight, male sterile, and pre-harvest sprouting tolerance have been modified in a few generations. Particularly, new haploid inducer lines have been created in maize, rice, Arabidopsis, wheat, alfalfa, foxtail millet, tomato, and Brassica oleracea by editing MTL (PLA1/NLD), DMP, and PLD3 genes via CRISPR/Cas9 for largely producing haploid grains directly. Additionally, new types of molecular markers have been developed and used to trace agronomically important traits for easily screening and locating gene position on chromosomes for gene cloning, except for generally employed makers like SSR, SNP, and EST.

Aegilops: Promising Genesources to Improve Agronomical and Quality Traits of Wheat

Aegilops: Promising Genesources to Improve Agronomical and Quality Traits of Wheat PDF Author: Peter Shewry
Publisher: Frontiers Media SA
ISBN: 2889639797
Category : Nature
Languages : en
Pages : 217

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Book Description
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Advances in Wheat Genetics: From Genome to Field

Advances in Wheat Genetics: From Genome to Field PDF Author: Yasunari Ogihara
Publisher: Springer
ISBN: 4431556753
Category : Science
Languages : en
Pages : 421

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Book Description
This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.

Molecular Breeding for Sustainable Crop Improvement

Molecular Breeding for Sustainable Crop Improvement PDF Author: Vijay Rani Rajpal
Publisher: Springer
ISBN: 3319270907
Category : Science
Languages : en
Pages : 484

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Book Description
The world population is estimated to reach to more than 10 billion by the year 2050. These projections pose a challenging situation for the agricultural scientists to increase crops productivity to meet the growing food demands. The unavailability and/or inaccessibility to appropriate gene pools with desired traits required to carry out genetic improvement of various crop species make this task formidable for the plant breeders. Incidentally, most of the desired genes reside in the wild genetic relatives of the crop species. Therefore, exploration and characterization of wild genetic resources of important crop species is vital for the efficient utilization of these gene pools for sustainable genetic improvements to assure food security. Further, understanding the myriad complexities of genic and genomic interactions among species, more particularly of wild relatives of crop species and/or phylogenetically distant germplasm, can provide the necessary inputs to increase the effectiveness of genetic improvement through traditional and/or genetic engineering methods. This book provides comprehensive and latest insights on the evolutionary genesis of diversity, access and its utilization in the evolution of various crop species. A comprehensive account of various crops, origin, exploitation of the primary, secondary and tertiary gene pools through breeding, biosystematical, cytogenetical and molecular phylogenetical relationships, and genetic enhancement through biotechnological interventions among others have been provided as the necessary underpinnings to consolidate information on the effective and sustainable utilization of the related genetic resources. The book stresses upon the importance of wild germplasm exploration, characterization and exploitation in the assimilation of important crop species. The book is especially intended for students and scientists working on the genetic improvement of crop species. Plant Breeders, Geneticists, Taxonomists, Molecular Biologists and Plant Biotechnologists working on crop species are going to find this book very useful.

Advances in Conservation and Utilization of Plant Genetic Resources

Advances in Conservation and Utilization of Plant Genetic Resources PDF Author: Svein Øivind Solberg
Publisher: Frontiers Media SA
ISBN: 2832553346
Category : Science
Languages : en
Pages : 299

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Book Description
Genetic diversity is the key to crop improvement and food security. There are more than 1500 gene banks around the world, and genetic resources are maintained in nature reserves and on farms. Genetic diversity serves as the starting point for breeding crops with improved nutritional quality, higher yields, and better tolerance to abiotic and biotic stresses. However, genetic diversity also provides opportunities for diversifying farm and food systems. Utilization depends on access to material and information. However, many gene banks experience backlogs in characterization, evaluation, regeneration, viability tests, plant health monitoring, and information sharing. This research topic focuses on advances in plant genetic resource conservation and utilization.

Cereal Genomics

Cereal Genomics PDF Author: Pushpendra K. Gupta
Publisher: Springer Science & Business Media
ISBN: 1402023596
Category : Technology & Engineering
Languages : en
Pages : 639

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Book Description
Cereals make an important component of daily diet of a major section of human population, so that their survival mainly depends on the cereal grain production, which should match the burgeoning human population. Due to painstaking efforts of plant breeders and geneticists, at the global level, cereal production in the past witnessed a steady growth. However, the cereal production in the past has been achieved through the use of high yielding varieties, which have a heavy demand of inputs in the form of chemical fertilizers, herbicides and insecticides/pesticides, leading to environmental degradation. In view of this, while increasing cereal production, one also needs to keep in mind that agronomic practices used for realizing high productivity do not adversely affect the environment. Improvement in cereal production in the past was also achieved through the use of alien genetic variation available in the wild relatives of these cereals, so that conservation and sustainable use of genetic resources is another important area, which is currently receiving the attention of plant breeders. The work leading to increased cereal production in the past received strong support from basic research on understanding the cereal genomes, which need to be manipulated to yield more from low inputs without any adverse effects as above. Through these basic studies, it also became fairly apparent that the genomes of all cereals are related and were derived from the same lineage, million of years ago.

Long Noncoding RNAs in Plants

Long Noncoding RNAs in Plants PDF Author: Santosh Kumar Upadhyay
Publisher: Academic Press
ISBN: 0128214635
Category : Science
Languages : en
Pages : 392

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Book Description
The growth of human population has increased the demand for improved yield and quality of crops and horticultural plants. However, plant productivity continues to be threatened by stresses such as heat, cold, drought, heavy metals, UV radiations, bacterial and fungal pathogens, and insect pests. Long noncoding RNAs are associated with various developmental pathways, regulatory systems, abiotic and biotic stress responses and signaling, and can provide an alternative strategy for stress management in plants. Long Noncoding RNAs in Plants: Roles in development and stress provides the most recent advances in LncRNAs, including identification, characterization, and their potential applications and uses. Introductory chapters include the basic features and brief history of development of lncRNAs studies in plants. The book then provides the knowledge about the lncRNAs in various important agricultural and horticultural crops such as cereals, legumes, fruits, vegetables, and fiber crop cotton, and their roles and applications in abiotic and biotic stress management. - Includes the latest advances and research in long noncoding RNAs in plants - Provides alternative strategies for abiotic and biotic stress management in horticultural plants and agricultural crops - Focuses on the application and uses of long noncoding RNAs