Microscopic Preparation Techniques for Plant Stem Analysis PDF Download
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Author: Holger Gärtner
Publisher:
ISBN: 9783941300767
Category : Microscopy
Languages : en
Pages : 78
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Book Description
Author: Holger Gärtner
Publisher:
ISBN: 9783941300767
Category : Microscopy
Languages : en
Pages : 78
Get Book Here
Book Description
Author: Sylvester Marsh
Publisher: Good Press
ISBN:
Category : Science
Languages : en
Pages : 91
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Book Description
In 'Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope' by Sylvester Marsh, readers are provided with a detailed and comprehensive guide on the techniques and processes involved in creating high-quality sections for microscopic analysis. The book covers the necessary tools, materials, and step-by-step instructions for obtaining clear and precise sections that are suitable for examination under the microscope. Written in a clear and instructional style, Marsh incorporates both scientific precision and practical tips for aspiring microscopists. This book serves as an invaluable resource for students, researchers, and professionals in the field of microscopy, offering critical insights into the art of section-cutting for in-depth scientific study. Sylvester Marsh, a renowned microscopist with years of experience in the field, brings his expertise to 'Section-Cutting', drawing from his own research and experiments to offer readers a wealth of knowledge on the subject. His passion for microscopy and dedication to advancing the field are evident throughout the book, making it a trusted source for anyone looking to master the art of section preparation for microscopic analysis. For anyone interested in honing their skills in section-cutting for microscopy, Sylvester Marsh's 'Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope' is a must-read. Whether you are a beginner or an experienced microscopist, this book will enhance your understanding of the techniques involved in creating high-quality sections, ultimately leading to more accurate and insightful observations under the microscope.
Author: Fritz H. Schweingruber
Publisher: Springer
ISBN: 3319735241
Category : Science
Languages : en
Pages : 207
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Book Description
This unique and attractive open access textbook combines the beauty of macroscopic pictures of plant stems with the corresponding colorfully stained images of anatomical micro-structures. In contrast to most botanical textbooks, it presents all the stem characteristics as photographs and shows the microscopic reality. The amount of text is reduced to a minimum, and the scientific information is highlighted with short legends and labeled photographs, allowing readers to focus on the pictures to easily understand how the anatomical structures relate to genetic, ecological, decomposition and technical influences. It includes a chapter devoted to simple anatomical preparation techniques, and further chapters showing the cell content, cell walls, meristematic tissues and stem structures of all major taxonomic units and morphological growth forms in various ecological and climatic regions from subarctic to equatorial latitudes, as well as structures of fossil, subfossil and technically altered wood. This textbook appeals to students and researchers in the fields of plant anatomy, taxonomy, ecology, dendrochronology, history, plant pathology, and evolutionary biology as well as to technologists.
Author: Edward Chee Tak Yeung
Publisher: Springer
ISBN: 3319199447
Category : Science
Languages : en
Pages : 572
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Book Description
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: František Baluška
Publisher: Springer Science & Business Media
ISBN: 0387360581
Category : Science
Languages : en
Pages : 331
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Book Description
This book covers cell-cell channels at all levels of biological organization. The purpose of this book is to document that cells are not physically separated and fully autonomous units of biological life as stated by the currently valid Cell Theory. If not the cell then some lower level unit must fulfill this role. The book deals also with the identity of this elusive unit of biological life.
Author: Fritz Hans Schweingruber
Publisher: Springer Nature
ISBN: 3030539768
Category : Science
Languages : en
Pages : 453
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Book Description
This is the first book to provide comprehensive information on the anatomy and ecology of arctic and alpine plants from cold sites around the globe, including representative species from Greenland, Iceland, Svalbard, Himalaya, Japan, Argentina, Ecuador and Western USA. It presents the study sites, including characteristic landscape and vegetation photographs. It also discusses species distribution, habitat preferences and features plant pictures, particularly focusing on the specific stem anatomical features, which differ in many cases from temperate zone herbs. Furthermore, each plant is characterized according to a newly constructed codification system. Based on the first author’s 20-years of field research, a close collaboration with numerous botanical gardens, and the vast ecological experience of the other authors, the book presents approximately 350 species. The general layout is comparable to Doležal et al’s 2018 book Anatomy, Age and Ecology of High Mountain Plants in Ladakh, the Western Himalaya.
Author: Patrick Echlin
Publisher: Springer Science & Business Media
ISBN: 0387857311
Category : Technology & Engineering
Languages : en
Pages : 329
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Book Description
Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Author: C Hawes
Publisher: Academic Press
ISBN: 0323148980
Category : Science
Languages : en
Pages : 477
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Book Description
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Author: Johannes Liesche
Publisher: Humana
ISBN: 9781493995615
Category : Science
Languages : en
Pages : 473
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Book Description
This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.
