Author: John Neal Brown
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 184
Book Description
Metabolic and Toxicological Studies in Channel Catfish (Ictalurus Punctatus)
Author: John Neal Brown
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 184
Book Description
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 184
Book Description
In Vitro Evaluation of the Channel Catfish Ictalurus Punctatus (Rafinesque) as a Test Species in Chemical Carcinogenesis Studies
Author: DE. Hinton
Publisher:
ISBN:
Category : Benzo[a]pyrene
Languages : en
Pages : 13
Book Description
The ability of isolated subcellular fractions and cells from the liver of channel catfish Ictalurus punctatus (Rafinesque) to perform key steps in carcinogenesis was studied using the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BP). Highpressure liquid chromatographic (HPLC) analysis of BP metabolism by catfish liver microsomal fraction revealed the formation of 9,10-diol BP; 4,5-diol BP; 7,8-diol BP; 9-OH BP; 3-OH BP; and quinones. When the microsomal fraction of catfish liver from fish pretreated with 3-methylcholanthrene was used, increased formation of 9,10-diol BP and 7,8-diol BP was seen. Postmitochondrial supernatant (S-9) of catfish liver incubated with required cofactors activated 2-acetylaminofluorene and BP into mutation-causing metabolites in a bacterial mutagen assay. Trypsin digestion of catfish liver was used to isolate 2.8 x 106 cells/g liver with 98 percent viability. Incubation of these cells after 1 day of culture with tritiated BP followed by autoradiographic localization showed preferential accumulation of BP over liver cell nuclei. Subsequent liquid scintillation analysis of cell fractions obtained by cesium chloride centrifugation revealed radioactivity in the deoxyribonucleic acid (DNA) fraction. Results of studies with microsomal fractions demonstrate the capacity of the liver mixed-function oxidase system (MFOS) of this species to metabolize carcinogens into mutagenic forms. Data with isolated cells indicate that activation reactions could result in the initiation of carcinogenesis within the intact liver and suggest the suitability of this species for carcinogen bioassay.
Publisher:
ISBN:
Category : Benzo[a]pyrene
Languages : en
Pages : 13
Book Description
The ability of isolated subcellular fractions and cells from the liver of channel catfish Ictalurus punctatus (Rafinesque) to perform key steps in carcinogenesis was studied using the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BP). Highpressure liquid chromatographic (HPLC) analysis of BP metabolism by catfish liver microsomal fraction revealed the formation of 9,10-diol BP; 4,5-diol BP; 7,8-diol BP; 9-OH BP; 3-OH BP; and quinones. When the microsomal fraction of catfish liver from fish pretreated with 3-methylcholanthrene was used, increased formation of 9,10-diol BP and 7,8-diol BP was seen. Postmitochondrial supernatant (S-9) of catfish liver incubated with required cofactors activated 2-acetylaminofluorene and BP into mutation-causing metabolites in a bacterial mutagen assay. Trypsin digestion of catfish liver was used to isolate 2.8 x 106 cells/g liver with 98 percent viability. Incubation of these cells after 1 day of culture with tritiated BP followed by autoradiographic localization showed preferential accumulation of BP over liver cell nuclei. Subsequent liquid scintillation analysis of cell fractions obtained by cesium chloride centrifugation revealed radioactivity in the deoxyribonucleic acid (DNA) fraction. Results of studies with microsomal fractions demonstrate the capacity of the liver mixed-function oxidase system (MFOS) of this species to metabolize carcinogens into mutagenic forms. Data with isolated cells indicate that activation reactions could result in the initiation of carcinogenesis within the intact liver and suggest the suitability of this species for carcinogen bioassay.
Toxaphene
Author: Foster L. Mayer
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 56
Book Description
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 56
Book Description
Pharmacokinetics and Metabolism of Sulfadimethoxine in Channel Catfish (Ictalurus Punctatus)
Author: Catherine M. F. Michel
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Toxicology Research Projects Directory
Author:
Publisher:
ISBN:
Category : Toxicology
Languages : en
Pages : 760
Book Description
Publisher:
ISBN:
Category : Toxicology
Languages : en
Pages : 760
Book Description
Acute Toxicity of Toxaphene to Fathead Minnows, Channel Catfish, and Bluegills
Author: W. Waynon Johnson
Publisher:
ISBN:
Category : Biological assay
Languages : en
Pages : 36
Book Description
Publisher:
ISBN:
Category : Biological assay
Languages : en
Pages : 36
Book Description
Studies on the Hepatic Microsomal Mixed Function Oxidase System of Channel Catfish (Ictalurus Punctatus, Rafinesque)
Author: Rhona M. Jack
Publisher:
ISBN:
Category : Cell metabolism
Languages : en
Pages : 230
Book Description
Publisher:
ISBN:
Category : Cell metabolism
Languages : en
Pages : 230
Book Description
Pharmacokinetic and Metabolic Profile of Trichlorfon in Channel Catfish (ictalurus Punctatus)
Author: Kedan Lin
Publisher:
ISBN:
Category :
Languages : en
Pages : 192
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 192
Book Description
Aquatic Toxicology and Hazard Assessment
Author:
Publisher: ASTM International
ISBN:
Category :
Languages : en
Pages : 475
Book Description
Publisher: ASTM International
ISBN:
Category :
Languages : en
Pages : 475
Book Description
Glutathione-dependent Metabolism in the Channel Catfish (Ictalurus Punctatus)
Author: Evan Patrick Gallagher
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 276
Book Description
Publisher:
ISBN:
Category : Channel catfish
Languages : en
Pages : 276
Book Description