Mass Spectrometry and Tandem Mass Spectrometry Analysis of Polymers and Polymer-protein Interactions PDF Download
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Author: Xiumin Liu
Publisher:
ISBN:
Category : Biomedical materials
Languages : en
Pages : 184
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Book Description
Tandem mass spectrometry (MS2) and ion mobility mass spectrometry (IM-MS) are powerful techniques to characterize biomaterials and polymers, and to provide structural information, on top of the compositional insight determined by single stage mass spectrometry (MS). In this study, collisionally activated dissociation (CAD) and electron transfer dissociation (ETD) were employed as MS2 fragmentation methods, and the resulting spectra were compared for specific compounds. The combination of MS2 with IM-MS was used for the characterization of protein conformation and the effect of polymers on protein conformational change. Chapter IV investigates the electron transfer dissociation (ETD) behavior of doubly charged ions with two different types of charges. Both CAD and ETD were used for the structural analysis of a protonated-sodiated peptide and sodiated-ammoniated polysaccharides. The advantages of ETD over CAD were documented with ETD spectra that showed more sequence-related fragment series for the peptide and revealed the location of the hydroxyethyl group on the pentamer of a polysaccharide. In Chapter V of this dissertation, the noncovalent interactions between proteins and polymers were investigated by electrospray ionization mass spectrometry (ESI-MS). Many synthetic polymers, when used for biomedical applications, come in contact with biological fluids. The polymers can significantly affect protein conformations in these fluids. The soft ionization feature of ESI enables the correlation between protein conformation and charge state distribution in the mass spectrum and the detection of protein-polymer complexes. ESI-MS data indicated that certain polysiloxanes can partially denature proteins. Chapter VI focuses on the precise structural characterization of branched glycopolymers. The two reactive sites on a mannose inimer, the polymerizable vinyl group and the homolytically cleavable halogen, enable the formation of hyperbranched architectures which are challenging to characterization. The presence of structural isomers in the sample was proposed based on the reaction mechanism of the synthesis. IM-MS and MS2 were employed as main analytical techniques in this study to unequirocally confirm the formation of branched structures.
Author: Xiumin Liu
Publisher:
ISBN:
Category : Biomedical materials
Languages : en
Pages : 184
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Book Description
Tandem mass spectrometry (MS2) and ion mobility mass spectrometry (IM-MS) are powerful techniques to characterize biomaterials and polymers, and to provide structural information, on top of the compositional insight determined by single stage mass spectrometry (MS). In this study, collisionally activated dissociation (CAD) and electron transfer dissociation (ETD) were employed as MS2 fragmentation methods, and the resulting spectra were compared for specific compounds. The combination of MS2 with IM-MS was used for the characterization of protein conformation and the effect of polymers on protein conformational change. Chapter IV investigates the electron transfer dissociation (ETD) behavior of doubly charged ions with two different types of charges. Both CAD and ETD were used for the structural analysis of a protonated-sodiated peptide and sodiated-ammoniated polysaccharides. The advantages of ETD over CAD were documented with ETD spectra that showed more sequence-related fragment series for the peptide and revealed the location of the hydroxyethyl group on the pentamer of a polysaccharide. In Chapter V of this dissertation, the noncovalent interactions between proteins and polymers were investigated by electrospray ionization mass spectrometry (ESI-MS). Many synthetic polymers, when used for biomedical applications, come in contact with biological fluids. The polymers can significantly affect protein conformations in these fluids. The soft ionization feature of ESI enables the correlation between protein conformation and charge state distribution in the mass spectrum and the detection of protein-polymer complexes. ESI-MS data indicated that certain polysiloxanes can partially denature proteins. Chapter VI focuses on the precise structural characterization of branched glycopolymers. The two reactive sites on a mannose inimer, the polymerizable vinyl group and the homolytically cleavable halogen, enable the formation of hyperbranched architectures which are challenging to characterization. The presence of structural isomers in the sample was proposed based on the reaction mechanism of the synthesis. IM-MS and MS2 were employed as main analytical techniques in this study to unequirocally confirm the formation of branched structures.
Author: Giorgio Montaudo
Publisher: CRC Press
ISBN: 1420037757
Category : Science
Languages : en
Pages : 601
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Book Description
Mass Spectrometry (MS) has rapidly become an indispensable tool in polymer analysis, and modern MS today complements in many ways the structural data provided by Nuclear Magnetic Resonance (NMR) and Infrared (IR) methods. Recent advances have sparked a growing interest in this field and established a need for a summary of progress made and results
Author: Minna Hakkarainen
Publisher: Springer Science & Business Media
ISBN: 3642280412
Category : Technology & Engineering
Languages : en
Pages : 219
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Book Description
Emerging Mass Spectrometric Tools for Analysis of Polymers and Polymer Additives, by Nina Aminlashgari and Minna Hakkarainen. Analysis of Polymer Additives and Impurities by Liquid Chromatography/Mass Spectrometry and Capillary Electrophoresis/Mass Spectrometry, by Wolfgang Buchberger and Martin Stiftinger. Direct Insertion Probe Mass Spectrometry of Polymers, by Jale Hacaloglu Mass Spectrometric Characterization of Oligo- and Polysaccharides and Their Derivatives, by Petra Mischnick. Electrospray Ionization-Mass Spectrometry for Molecular Level Understanding of Polymer Degradation, by Minna Hakkarainen.
Author: Liang Li
Publisher: John Wiley & Sons
ISBN: 0470567228
Category : Science
Languages : en
Pages : 325
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Book Description
Principles and Practices of Polymer Mass Spectrometry helps readers acquire the skills necessary for selecting the optimal methods, handling samples, analyzing the data, and interpreting the results of the mass spectrometry of polymers. This guide describes the principles of polymer MS and best practices in polymer characterization. It discusses different approaches, including MALDI, ESI, TOF MS, and FT-MS. It provides a guide to developing appropriate sample preparation protocols for different polymers. Complete with examples of applications and experiments, this is an excellent reference for scientists, researchers, graduate students, and others.
Author: Yuan Gao
Publisher:
ISBN:
Category : Bioconjugates
Languages : en
Pages : 66
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Book Description
Mass spectrometry (MS) is increasingly used for the characterization of polymers and supramolecular protein-polymer bioconjugates; especially after the introduction of soft ionization methods such as electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Regular MS measures molecular weight information by detecting the mass-to-charge (m/z) ratio. However, if analyzing the structure of a complicated molecule such as a protein-polymer bioconjugate, information besides the molecular mass is necessary. Tandem MS (MS/MS), which involves isolation and fragmentation of a precursor ion, is widely used to gain more detailed structural information. MALDI-MS is a powerful technique for the analysis of polymers. One of the most important steps for MALDI analysis is sample preparation. MALDI sample preparation aims of producing densely packed microcrystals that improve mass accuracy, resolution, and shot-to-shot reproducibility as compared to large crystals. This thesis compared three common sample preparation methods for MALDI analysis: Dried droplet, solvent-free MALDI (SF-MALDI), and surface-layer MALDI (SL-MALDI). Additionally, ESI-MS and ESI-MS/MS were used to characterize supramolecular protein-polymer bioconjugates. Acidic polymers such as poly(styrene sulfonate) (PSS) were used to form non-covalent complexes with ubiquitin, a protein with many basic amino acid binding sites. Based on studies with simple peptides, MS/MS fragmentation of the ubiquitin complexes should cause cleavages in the protein backbone but preserve the non-covalent interaction. This study will apply a method to determine the attachment site of the polymer, which would reveal the surface-accessible basic amino acid residues and the higher-order structure of ubiquitin.
Author: Kevin Downard
Publisher: John Wiley & Sons
ISBN: 047014632X
Category : Science
Languages : en
Pages : 153
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Book Description
The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.
Author: Christopher Barner-Kowollik
Publisher: John Wiley & Sons
ISBN: 3527641831
Category : Science
Languages : en
Pages : 501
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Book Description
Combining an up-to-date insight into mass-spectrometric polymer analysis beyond MALDI with application details of the instrumentation, this is a balanced and thorough presentation of the most important and widely used mass-spectrometric methods. Written by the world's most proficient experts in the field, the book focuses on the latest developments, covering such technologies and applications as ionization protocols, tandem and liquid chromatography mass spectrometry, gas-phase ion-separation techniques and automated data processing. Chapters on sample preparation, polymer degradation and the usage of mass-spectrometric tools on an industrial scale round off the book. As a result, both entrants to the field and experienced researchers are able to choose the appropriate methods and instrumentations -- and to assess their respective strengths and limitations -- for the characterization of polymer compounds.
Author: Harald Pasch
Publisher: Springer Science & Business Media
ISBN: 3662050463
Category : Science
Languages : en
Pages : 312
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Book Description
MALDI-TOF mass spectrometry is one of the latest and most fascinating new developments in the analysis of organic compounds. Originally developed for the analysis of biomolecules, it has developed into one of the most powerful techniques for the characterization of synthetic polymers. This book describes the fundamentals of the MALDI process and the technical features of MALDI-TOF instrumentation. It reviews the application of MALDI-TOF for identification, chemical and molar mass analysis of synthetic polymers. With many examples, the monograph examines experimental protocols for the determination of endgroups, the analysis of copolymers and additives, and the coupling of liquid chromatography and MALDI-TOF in detail.
Author: Asoka Ranasinghe
Publisher:
ISBN:
Category : Ionization
Languages : en
Pages : 264
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Book Description
Author: Jean-François Lutz
Publisher: John Wiley & Sons
ISBN: 3527342370
Category : Technology & Engineering
Languages : en
Pages : 532
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Book Description
Edited by a leading authority in the field, the first book on this important and emerging topic provides an overview of the latest trends in sequence-controlled polymers. Following a brief introduction, the book goes on to discuss various synthetic approaches to sequence-controlled polymers, including template polymerization, genetic engineering and solid-phase chemistry. Moreover, monomer sequence regulation in classical polymerization techniques such as step-growth polymerization, living ionic polymerizations and controlled radical polymerizations are explained, before concluding with a look at the future for sequence-controlled polymers. With its unique coverage of this interdisciplinary field, the text will prove invaluable to polymer and environmental chemists, as well as biochemists and bioengineers.
