Author:
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ISBN:
Category :
Languages : en
Pages : 0
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Book Description
The purpose of this grant is to define interactions between stroma and epithelium in mammary oncogenesis in mice transgenic for Shope growth factor (SGF). Different lines of SGF transgenic mice express this EGF-like cytokine using inducible (metallothionein, MT) or constitutive (RSV-LTR) promoters. For the year now ending, most stated goals have been met, although some remain. We made sufficient SGF-transgenic mice of RSV-SGF and MT-SGF lines to analyze mammary differentiation and oncogenesis, including testing SGF expression and that of particular cellular genes that are important in these processes. It also induced defining which cells express the transgene and these cellular genes. We have also interbred RSV- and MT-SGF transgenic mice with p53-/- mice, to determine the nature of the interactions between homozygous deletion of p53 tumor suppressor gene and SGF expression as regards mammary gland development and tumorigenesis. These mice have been analyzed, and additional numbers of them are being produced. Our study of SGF activity in cultured cells has been progressing more slowly, and these studies are largely still in progress. Thus, our studies of the effects of SGF on the murine mammary gland are proceeding, and additional work is in progress.
Author: Young Chul Kim
Publisher:
ISBN:
Category :
Languages : en
Pages : 154
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Book Description
Author: Itzhak D. Goldberg
Publisher: Birkhäuser
ISBN: 3034890702
Category : Science
Languages : en
Pages : 304
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Book Description
The contribution of epithelia-mesenchyme interaction to normal development (eg., tissue formation) and to neoplasia has become a subject of increasing interest to scientists because of recent progress in deciphering the molecular signals that mediate this interaction. Clearly, some of the same types of molecules (eg., growth factors and their receptors, proteolytic enzymes, cell adhesion molecules, and structural proteins of the extracellular matrix) mediate exchange of information between epithelia and mesenchyme during normal development and malignant growth. However, defects in the regulation of this exchange appear to contribute to malignancy by allowing growth promoting, invasogenic, and angiogenic factors to accumulate within the microenvironment of the tumor. For example, recent studies suggest that abnormal interactions between tumor epithelial cells and stromal mesenchymal cells contribute to the overproduction and accumulation of scatter factor (hepatocyte growth factor), an invasogenic and angiogenic cytokine, in certain types of tumor. The production and and activation of type IV collagenase, a matrix-degrading enzyme required for tumor cell invasion, appears to require intimate cooperation between tumor and stromal cells. The material contained in this volume highlights the state-of-the-art of knowledge of the molecular mechanisms by which epithelia and mesenchyme collaborate, and the abnormalities in these mechanisms that may lead to the development of cancer.
Author: E. Anderson
Publisher: Springer Science & Business Media
ISBN: 1461309433
Category : Science
Languages : en
Pages : 519
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Book Description
The idea for this book arose during the 1985 Gordon Conference on "Mammary Gland Biology". New developments in the methodology of cell biology and the explosive growth of molecular biology had begun to impact upon our understanding of mammary gland growth and function. It seemed a propitious time for summarizing the current status of knowledge of the cell and molecular biology of mammary cancer and for attempting to outline future areas of concern and interest. The reviews presented here were completed by the Fall of 1986. Although new insights will surely continue to emerge, it is hoped that the material in this volume will form not only a current update but a basic core of information for future experiments. We have not attempted to cover all areas of mammary gland transformation. Those areas where recent detailed reviews are already available have been omitted. Also, the areas of normal gland development, cell ultrastructure, hormone responsiveness, chemotherapy and clinical aspects of mammary cancer have not been included. Instead, we have selected those areas where the development of new methodology, reagents and results have led to new ideas about mammary gland function and development as they are related to neoplasia.
Author: Bryony Wiseman
Publisher:
ISBN:
Category :
Languages : en
Pages : 18
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Book Description
The mammary gland relies on stromal-epithelial interactions for proper development. Tumorigenesis occurs when these signals are misinterpreted. Here we show that stromal matrix metalloproteinases (MMPs) have distinct roles in mammary gland branching morphogenesis. MMP activity is required for normal mammary gland development since mice treated with a synthetic MMP inhibitor, GM6OOl, have retarded ductal development. Furthermore, 3D cultures of mammary organoids also require MMP activity to branch in response to growth factors, indicating a mammary specific response. We also show that specific MMPs are required for distinct aspects of mammary gland morphogenesis. Specifically, MMF-3 is needed for branching because MMP-3 null mice have significantly fewer branches than controls. However, the ductal length is normal. In contrast, MMP-2 is required for ductal elongation, since the ducts of MMP-2 null mice are retarded in their penetration into the fat pad. However, the branching of the MMP-2 null ducts is normal. MMP-l4 is a known activator of MMP-2 and we show it is highly expressed in front of terminal end buds. Mammary glands deficient in MMP-l4 grown under the kidney capsule of nude mice also have defective ductal morphogenesis. Thus, these stromal enzymes are important, and have distinct roles, in patterning the mammary gland.
Author: Margareta M. Mueller
Publisher: Springer Science & Business Media
ISBN: 9400706596
Category : Medical
Languages : en
Pages : 453
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Book Description
During the last 20 years it has become increasingly clear that the tumor micro-environment, the tumor stroma with its cellular end extracellular components, plays an crucial role in regulating tumor growth and progression. This book on “Tumor-associated fibroblasts and their matrix” as part of the series on “Tumor-Microenvironment” is the first comprehensive discussion of these two main players of the tumor microenvironment. The best experts in this new area of tumor research and therapy review the role of these major components in the tumor stroma in the process of tumor development and progression. They discuss their interaction with other players such as blood vessels and immune cells, and show novel perspectives for tumor therapy. This compilation of excellent contributions of the best known experts in this important field in cancer research and therapy is a must for all scientists engaged in basic and clinical research. Increasing evidence of successful targeting of both cellular and matrix components in tumor therapy renders this book of particular interest for scientists engaged in pharmaceutical industry searching for new components for cancer therapy.
Author: Robert B. Dickson
Publisher: Springer Science & Business Media
ISBN: 1461312590
Category : Medical
Languages : en
Pages : 432
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Book Description
Mammary Tumor Cell Cycle, Differentiation and Metastasis is the fifth volume since 1988 in a series designed to broadly examine current advances in the cellular and molecular biology of breast cancer. As in previous volumes, the editors have invited recognized experts in cutting-edge topics to provide a chapter focused on their area of research. The editors have turned to the researchers who study rodent models of the disease and to those who study the cellular and molecular basis of human breast cancer. The first section of the book is devoted to new mouse models of mammary development and tumorigenesis. The second section moves to studies of human breast cancer and focuses on receptors, signalling, and the cell cycle. The final section deals with defective tissue interactions in human breast cancer. We are now in a period of extremely rapid accumulation of data on the molecular and cellular biology of breast cancer. These findings are highlighted in chapters from Mammary Tumor Cell Cycle, Differentiation and Metastasis: Advances in Cellular and Molecular Biology of Breast Cancer.
Author: Stephanie Chantal Lebret
Publisher:
ISBN:
Category : Breast
Languages : en
Pages : 472
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Book Description
This study determined that the microenvironment influences mammary cellular development and that this microenvironment differs between normal and malignant tissue. Cancer-associated fibroblasts, loss of an extracellular protein and presence of a growth factor were demonstrated to influence cancer cell migration, presenting a basis for the understanding of breast cancer metastasis.
Author: Robert B. Dickson
Publisher: Springer
ISBN:
Category : Health & Fitness
Languages : en
Pages : 384
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Book Description
The current volume represents the fourth over a period of five years in our series on Advances in the Cellular and Molecular Biology of Breast Cancer. The first three volumes were entitled Breast Cancer: Cellular and Molecular Biology, Regulatory Mechanisms in Breast Cancer, and Genes, Oncogenes, and Hormones, respectively. Throughout this series, we have tried to take a broad look at cutting-edge topics in basic science research into breast cancer. This attempt has resulted in a wide range of subject material, including rodent and human model systems, oncogenes, suppressor genes, growth factors, hormones, tumor-host interactions, and determinants of metastases. Since our last volume, research in breast cancer has continued to proceed at an explosive rate. We hope the current volume will provide the reader with some of the excitement felt by the editors and authors as we begin to understand this all-too-common disease. The first section of this book is devoted to the basic processes of proli feration, differentiation, and malignant progression of breast cancer. T.l. Anderson and W.R. Miller lead off with a detailed description of controls on proliferation in the normal human breast and in breast cancer. This chapter strongly emphasizes pathological aspects. The second chapter, by M.R. Stampfer and P. Yaswen, presents a corresponding viewpoint through a presentation of experiments with human mammary epithelial cells in culture. The second section of the book emphasizes the genetic basis for breast cancer onset and malignant progression. Chapter 3, by M.-C. King and S.
Author: Andrea Manni
Publisher: Springer Science & Business Media
ISBN: 1592596991
Category : Medical
Languages : en
Pages : 387
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Book Description
A cutting-edge review of how derangements in the hormonal and growth factor mechanisms controlling normal mammary development lead to breast cancer. Drawing on the multidisciplinary expertise of leading authorities, the book highlights the roles of oncogenes and tumor suppressor genes, spelling out the importance of autocrine/paracrine loops (e.g., stromal epithelial interactions) in supporting breast cancer cell proliferation and the progression to hormone independence. The book's many prominent contributors also illuminate significant recent advances in the biochemistry and physiology of hormone receptors and review the state-of -the-art in the endocrine therapy of breast cancer. Endocrinology of Breast Cancer provides a unique integrated overview of the most significant basic and clinical developments concerning the hormonal aspects of breast cancer.
