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Author: Knox Van Dyke
Publisher: CRC Press
ISBN: 9780849358654
Category : Medical
Languages : en
Pages : 356
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Book Description
This unique, one-of-a-kind book provides updated information on the various aspects of luminescence immunoassay (LIA). Focusing on this newly emerging technology, this work describes the important findings regarding the chemistry of light and the various forms of luminescent immunoassay. It includes antigen labeling, enzyme labeling, cofactor labeling, enzyme multiplied assay, and multiple labeled immunoassay. This interesting text also discusses calculations, including computer programs available for high throughput analysis. No other compendium provides such a broad overview, as well as details on this fascinating subject. Both novices and experts will find important and useful information packed in this must-have resource on luminescence immunoassay.
Author: Knox Van Dyke
Publisher: CRC Press
ISBN: 9780849358654
Category : Medical
Languages : en
Pages : 356
Get Book
Book Description
This unique, one-of-a-kind book provides updated information on the various aspects of luminescence immunoassay (LIA). Focusing on this newly emerging technology, this work describes the important findings regarding the chemistry of light and the various forms of luminescent immunoassay. It includes antigen labeling, enzyme labeling, cofactor labeling, enzyme multiplied assay, and multiple labeled immunoassay. This interesting text also discusses calculations, including computer programs available for high throughput analysis. No other compendium provides such a broad overview, as well as details on this fascinating subject. Both novices and experts will find important and useful information packed in this must-have resource on luminescence immunoassay.
Author: I. Weeks
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 320
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Book Description
Chemiluminescence immunoassay is now established as one of the best alternatives to conventional radioimmunoassay for the quantitation of low concentrations of analytes in complex samples. During the last two decades the technology has evolved into analytical procedures whose performance far exceeds that of immunoassays based on the use of radioactive labels. Without the constraints of radioactivity, the scope of this type of analytical procedure has widened beyond the confines of the specialist clinical chemistry laboratory to other disciplines such as microbiology, veterinary medicine, agriculture, food and environmental testing. This is the first work to present the topic as a subject in its own right. In order to provide a complete picture of the subject, overviews are presented of the individual areas of chemiluminescence and immunoassay with particular emphasis on the requirements for interfacing chemiluminescent and immunochemical reactions. The possible ways of configuring chemiluminescence immunoassays are described. State-of-the-art chemiluminescence immunoassay systems are covered in detail together with those systems which are commercially available. The book is aimed at researchers and routine laboratory staff in the life sciences who wish to make use of this high-performance analytical technique and also at those interested in industrial applications of the technology in the food, agricultural and environmental sciences.
Author: Anthony K. Campbell
Publisher: Wiley-VCH
ISBN:
Category : Science
Languages : en
Pages : 624
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Book Description
Describes the occurrence, chemistry, and measurement of chemiluminescence, a phenomenon that together with the use of very sensitive light detectors has led to a number of biomedical applications and new concepts about disease processes. Covers the principles of using the reactions from fireflies and luminous bacteria to measure enzymes and metabolites; calcium-sensitive proteins from luminous jellyfish to measure changes in calcium inside living cells; the measurement of oxygen radicals and the replacement of radioactive labels in immunoassay by chemiluminescent compounds; and future applications in research and clinical laboratories. Acidic paper. Annotation copyrighted by Book News, Inc., Portland, OR
Author:
Publisher: Academic Press
ISBN: 9780080566283
Category : Science
Languages : en
Pages : 388
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Book Description
Advances in Clinical Chemistry
Author: James F. Case
Publisher: World Scientific
ISBN: 9789812811158
Category : Science
Languages : en
Pages : 548
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Book Description
Light-emitting reactions occur in some living organisms, and are also now extensively exploited by industry and various branches of biomedical science. Luminescence from the natural world, particularly from marine organisms, is increasingly being harnessed by genetic and chemical manipulation to enhance the quality of human life. This volume contains cutting-edge contributions from most of the world's leading researchers in this field. It presents an up-to-date compilation of the range of biomedical, strategic and ecological applications of chemiluminescence and bioluminescence. It documents and highlights the rapid advance in knowledge concerning both the mechanisms and the uses of luminescence, and covers all the important developments of recent years. Contents: Marine Bioluminescence; Firefly Bioluminescence; Chemiluminescence; Applications of Bioluminescence; Applications of Chemiluminescence; Immunoassay and DNA Probe Assays; Cellular Luminescence; Reporter Genes in Cell Biology and Analysis; Luminescence in Science and Education; Instrumentation and Imaging of Luminescence. Readership: Biomedical specialists, biochemists, marine biologists and geneticists.
Author: Danny L. Wiedbrauk
Publisher: Elsevier
ISBN: 0080536891
Category : Science
Languages : en
Pages : 405
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Book Description
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables
Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 1475730616
Category : Science
Languages : en
Pages : 708
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Book Description
`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface
Author: Chris D. Geddes
Publisher: John Wiley & Sons
ISBN: 0470642785
Category : Science
Languages : en
Pages : 655
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Book Description
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Author: Akio Tsuji
Publisher: World Scientific
ISBN: 9814481211
Category : Science
Languages : en
Pages : 596
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Book Description
Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence. The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays. The proceedings have been selected for coverage in: • Biochemistry & Biophysics Citation Index™ • Chemistry Citation Index™ • Index to Scientific & Technical Proceedings® (ISTP® / ISI Proceedings) • Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings) • CC Proceedings — Engineering & Physical Sciences • CC Proceedings — Biomedical, Biological & Agricultural Sciences Contents:Bioluminescence and Mating Behavior in Pony Fish, Leiognathus nuchalis (N Azuma et al.)Importance of Firefly Luciferase C-terminal Domain in Binding of Luciferyl-Adenylate (K Ayabe et al.)Effect of Oxygen and Hydrogen Ion on the Modulation of the Bioluminescence from Luminous Bacteria (H Karatani et al.)Superoxide or Singlet Oxygen: The Chemiluminescence of Cypridina Luciferin Analogues in Photodynamic Solutions (M Bancírová & I Šnyrychová)On the Role of Singlet-Oxygen Dimol Chemiluminescence in Dioxirane Reactions (W Adam et al.)On the CIEEL Mechanism of Triggerable Dioxetanes: Does the Electron Jump or Is It Charge Transfer? (W Adam & A V Trofimov)Single-Molecule Imaging of Protein in Living Cells by Pin-Fiber Video-Microscopy (Y Hirakawa et al.)Construction of a Novel Bioluminescence Bacterial Biosensor for Real-Time Monitoring of Cytotoxic Drugs Activity (H M Alloush et al.)The Chemiluminescent Measurement of the Black and Green Tea Antioxidant Capacity and the Comparison with Their Antimicrobial Activity (M Bancírová & I Šnyrychová)Use of Bioluminescent Salmonella typhimurium DT104 to Monitor Uptake and Intracellular Survival Within a Human Cell-Line (J E Angell et al.)Tandem Bioluminescent Enzyme Immunoassay for BDNF and NT-4/5 (S Akahane et al.)Use of the Peroxyoxalate Chemiluminescent Reaction in Acetone in the Presence of Nile Red for the Analysis of Glucose (P Castro-Hartmann et al.)A New Assay for Determining Pyrophosphate Using Pyruvate Phosphate Dikinase and Its Application to DNA Analysis (H Arakawa et al.)and other papers Readership: Scientists in basic luminescene research, analytical chemists and biochemists. Keywords:Chemiluminescene;Bioluminescence;Luciferase;Luciferin;ATP;Bioanalysis;Green Fluroscent Protein (GFP);Imaging;Clinical AnalysisKey Features:Up-to-date coverage of the latest developments in bioluminescence and chemiluminescenceComprehensive coverage of fundamental and applied aspects of bioluminescence and chemiluminescenceLatest experimental procedures and protocols in bioluminescence and chemiluminescence
Author: Fan Xia
Publisher: Springer
ISBN: 9811078351
Category : Science
Languages : en
Pages : 216
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Book Description
This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
