Isolation of CDNA Clones and Patterns of Expression of Genes Coding for Enzymes of the Carotenoid Biosynthetic Pathway in Carrot (Daucus Carota L.) PDF Download
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Author: Maria Esther de Noronha Fonseca Boiteux
Publisher:
ISBN:
Category :
Languages : en
Pages : 252
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Book Description
Author: Maria Esther de Noronha Fonseca Boiteux
Publisher:
ISBN:
Category :
Languages : en
Pages : 252
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Book Description
Author: M. E. de N. FONSECA-BOITEUX
Publisher:
ISBN:
Category :
Languages : en
Pages : 251
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Book Description
Objetivos: Other potential consequences of the molecular characterization of carotenoid genes in carrot include map-based associations between carotenoid genes/alleles and root color phenotypes, expression studies at RNA level, isolation of tissue-specific promoters, and genetic manipulation via transgenic approaches.
Author: Brian James Just
Publisher:
ISBN:
Category :
Languages : en
Pages : 252
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Book Description
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Category : Dissertations, Academic
Languages : en
Pages : 810
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Author:
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ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 816
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Author: Carlos Antonio Fernandes Santos
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
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Book Description
Orange carrots are a top ranked vegetable in terms ofpro-vitamin A content. Carotenoid pathway products were investigated in F2 populations from two different carrot crosses: orange Brasilia x dark orange HCM and orange B493 x white wild QAL. Broad sense heritabilities va1ues for a1l carotenoids were greater than 90% in the B493 x QAL cross and from 35% to 70% among different carotenoid,s in the Brasilia x HCM cross. The estimated number of factors was 4 for a-carotene, 3 for ~-carotene and total carotenes and one for ?- carotene, lycopene and phytoene in the orange x dark orange cross, and 4 for a-carotene, 1-2 for lycopene and tota1 carotenes and 1 for the other carotenes in the orange x white cross. In comparison to the known biochemical pathways the correct order of substrates and products, phytoene-+? -carotene-+ lycopene, was identified in the path analysis of f3-carotene in the cross Brasilia x HCM but not in the correlation analysis. Linkage grouping ana1ysis assigned 287 and 250 scored molecular markers to the nine chromosomes of carrots, at LOD scores ranging from 3.0 to 7.0 and the average marker spacing was 4.78,4.80,5.54 and 5.13 cM in 11 the Brasilia-, HCM-, B493 and QAL-coupling phase maps, respectively. Interval mapping performed with the orange x dark orange cross detected four, eight, three, one, five and three putative QTL associated with accumulation of ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and total carotenoids, respectively, with major QTL explaining from 10.2 to 13.0% of total phenotypic variation. In the B493 x QAL population single marker analysis identified loci explaining 13.8%,6.8%, 19.3%,5.7%, 17.5% and 20.2% of total phenotypic variation for ?-carotene, a-carotene, 13-carotene, lycopene, phytoene and tota1 carotenoids content, respectively. Overall analysis showed clustered loci affecting the phenotypic variation of carotenoidpathway suggesting clusters of related-pathway loci as an evolutionary mechanism and supporting an adaptive evolutionary model suggested by H. A. Orr. Path analysis and QTL studies suggested that phytoene biosynthesis, perhaps associated with a root specific signal, are the two key factors limiting the carotenoid pathway in roots of white carrots.
Author: Claudia Stange
Publisher: Springer
ISBN: 3319391267
Category : Science
Languages : en
Pages : 454
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Book Description
This comprehensive, edited book explores carotenoids and their important functional roles in yeast, bacteria and plants and a profound exposition on the structures of carotenoid molecules, focusing in the first of three parts on the biosynthesis of carotenoids. The regulation of carotenoid biosynthesis in photosynthesis as well as in plant, fruits, storage roots and algae is central to the second part, and discoveries about the function of carotenoids in human health feature in the third and final part. Many helpful illustrations, explanations, overviews and examples help to bring readers up to date on relevant themes including carotenogenic genes, carotenoids in fruits and metabolic engineering. The book explores where carotenoids are synthesized in nature, including in carrots and algae. Contributing expert authors examine enzyme functions and plant models, and analyze the structure of carotenoid molecules. The function of carotenoids in photosynthesis and in photosynthetic organs as well as during fruit ripening are then explored. A whole chapter is dedicated to the latest research on apocarotenoids and further chapters cover interesting and novel themes on plastid development and the epigenetic regulation that affects carotenoid synthesis in plants. The metabolic engineering of carotenoids that has been done in fruits, plants, and seeds is another area that readers can explore, along with evidences on the function of carotenoids in human nutrition, as antioxidants, as in the control of lipid metabolism and in the absorption of carotenoids. This is a highly informative and wide-ranging work which will update researchers in the field, as well as supporting students of plant physiology and biotechnology, as supplementary reading.
Author: Isil Aksan Kurnaz
Publisher: CRC Press
ISBN: 1482260905
Category : Medical
Languages : en
Pages : 334
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Book Description
Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and
Author: Kapuganti Jagadis Gupta
Publisher: John Wiley & Sons
ISBN: 1118790464
Category : Science
Languages : en
Pages : 398
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Book Description
Rapid developments in molecular and systems biology techniques have allowed researchers to unravel many new mechanisms through which plant cells switch over to alternative respiratory pathways. This book is a unique compendium of how and why higher plants evolved alternative respiratory metabolism. It offers a comprehensive review of current research in the biochemistry, physiology, classification and regulation of plant alternative respiratory pathways, from alternative oxidase diversity to functional marker development. The resource provides a broad range of perspectives on the applications of plant respiratory physiology, and suggests brand new areas of research. Other key features: written by an international team of reputed plant physiologists, known for their pioneering contributions to the knowledge of regular and alternative respiratory metabolism in higher plants includes step-by-step protocols for key molecular and imaging techniques advises on regulatory options for managing crop yields, food quality and environment for crop improvement and enhanced food security covers special pathways which are of key relevance in agriculture, particularly in plant post-harvest commodities Primarily for plant physiologists and plant biologists, this authoritative compendium will also be of great value to postdoctoral researchers working on plant respiration, as well as to graduate and postgraduate students and university staff in Plant Science. It is a useful resource for corporate and private firms involved in developing functional markers for breeding programs and controlling respiration for the prevention of post-harvest losses in fruit, vegetables, cut flowers and tubers.
Author: Anne E. Osbourn
Publisher: Springer Science & Business Media
ISBN: 0387854983
Category : Science
Languages : en
Pages : 588
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Book Description
Plants produce a huge array of natural products (secondary metabolites). These compounds have important ecological functions, providing protection against attack by herbivores and microbes and serving as attractants for pollinators and seed-dispersing agents. They may also contribute to competition and invasiveness by suppressing the growth of neighboring plant species (a phenomenon known as allelopathy). Humans exploit natural products as sources of drugs, flavoring agents, fragrances and for a wide range of other applications. Rapid progress has been made in recent years in understanding natural product synthesis, regulation and function and the evolution of metabolic diversity. It is timely to bring this information together with contemporary advances in chemistry, plant biology, ecology, agronomy and human health to provide a comprehensive guide to plant-derived natural products. Plant-derived natural products: synthesis, function and application provides an informative and accessible overview of the different facets of the field, ranging from an introduction to the different classes of natural products through developments in natural product chemistry and biology to ecological interactions and the significance of plant-derived natural products for humans. In the final section of the book a series of chapters on new trends covers metabolic engineering, genome-wide approaches, the metabolic consequences of genetic modification, developments in traditional medicines and nutraceuticals, natural products as leads for drug discovery and novel non-food crops.
