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Author: Kelli Janae Mathiason
Publisher:
ISBN:
Category :
Languages : en
Pages : 164
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Book Description
Author: Kelli Janae Mathiason
Publisher:
ISBN:
Category :
Languages : en
Pages : 164
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Author: Elaine Lynnette Philbrick
Publisher:
ISBN:
Category :
Languages : en
Pages : 164
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Book Description
Author: Avni Bhawan Santani
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Stallion fertility is of significant economic importance in the multibillion dollar equine industry. Presently, the underlying genetic causes of infertility in stallions are unknown. Analysis of the human genome has shown that in more than 25% of cases, male infertility is associated with deletions/rearrangements in the Y chromosome. Presently there is no gene map for the Y chromosome in the horse. Therefore, the primary aim of this study is to build a detailed physical map of the chromosome with a long-term aim to identify and analyze Y-specific factors affecting fertility in stallions. Tomaterialize this, we constructed the first radiation hybrid and FISH map of the euchromatic region of the horse Y chromosome. This basic map was used to obtain Y-specific BAC clones that provided new STS markers from the end sequences. Chromosome walking provided 73 BACs comprising 7 contigs that were built across the euchromatic region using 124 markers for content mapping. The results were validated by restriction fingerprinting and Fiber FISH. The map is presently the most informative among the domestic species and second to only human and mouse Y chromosome maps. The construction of this map will pave the way for isolation and functional characterization of genes critical for normal male fertility and reproduction and will in the future lead to the development of a diagnostic test to facilitate early identification of deletions/rearrangements on the Y chromosome of potentially affected foals/stallions. The second part of the study comprised the first extended investigation to assess genetic variation in the horse Y chromosome. Approximately 4.5Mb of the euchromatic region was screened for polymorphic microsatellite markers. Of the 27 markers that were characterized and screened for polymorphism in 14 breeds of the domestic horse and eight extant equids, only one was polymorphic in the domestic horse, suggesting a low level of genetic variation on the chromosome. However, 21 of the markers showed noteworthy variation (on average four alleles/marker) among the eight equids. These markers will be vital in future studies aimed at elucidating the genetic relationships between the various equids through phylogenetic analysis.
Author: Hans Ellegren
Publisher:
ISBN: 9789157647108
Category : Cartes chromosomiques
Languages : en
Pages : 84
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Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1860
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Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 2432
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Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Author:
Publisher:
ISBN:
Category : Genetics
Languages : en
Pages : 514
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Author: Bing-Yuan Chen
Publisher: Springer Science & Business Media
ISBN: 1592591779
Category : Science
Languages : en
Pages : 429
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PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Author: Bhanu P. Chowdhary
Publisher: John Wiley & Sons
ISBN: 0813815630
Category : Science
Languages : en
Pages : 349
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Book Description
Analysis of the equine genome began just over a decade ago, culminating in the recent complete sequencing of the horse genome. The availability of the equine whole genome sequence represents the successful completion of an important era of equine genome analysis, and the beginning of a new era where the sequence information will catalyze the development of new tools and resources that will permit study of a range of traits that are economically important and are significant to equine health and welfare. Equine Genomics provides a timely comprehensive overview of equine genomic research. Chapters detail key accomplishments and the current state of research, as well as looking forward to possible applications of genomic technologies to horse breeding, health, and welfare. Equine Genomics delivers a global overview of the topic and is seamlessly edited by a leading equine genomics researcher. Equine Genomics is an indispensible source of information for anyone with an interest in this increasingly important field of study, including equine genomic researchers, clinicians, animal science professionals and equine field veterinarians.
Author: Jeffrey Bennetzen
Publisher: Springer
ISBN: 3319974270
Category : Science
Languages : en
Pages : 390
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Book Description
This book discusses advances in our understanding of the structure and function of the maize genome since publication of the original B73 reference genome in 2009, and the progress in translating this knowledge into basic biology and trait improvement. Maize is an extremely important crop, providing a large proportion of the world’s human caloric intake and animal feed, and serving as a model species for basic and applied research. The exceptionally high level of genetic diversity within maize presents opportunities and challenges in all aspects of maize genetics, from sequencing and genotyping to linking genotypes to phenotypes. Topics covered in this timely book range from (i) genome sequencing and genotyping techniques, (ii) genome features such as centromeres and epigenetic regulation, (iii) tools and resources available for trait genomics, to (iv) applications of allele mining and genomics-assisted breeding. This book is a valuable resource for researchers and students interested in maize genetics and genomics.
