Human Retrovirus Protocols PDF Download
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Author: Tuofu Zhu
Publisher: Springer Science & Business Media
ISBN: 1592599079
Category : Science
Languages : en
Pages : 488
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Book Description
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Author: Tuofu Zhu
Publisher: Springer Science & Business Media
ISBN: 1592599079
Category : Science
Languages : en
Pages : 488
Get Book Here
Book Description
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Author: Peter Portegies
Publisher: Newnes
ISBN: 0444520104
Category : Medical
Languages : en
Pages : 386
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Book Description
This volume provides a comprehensive understanding of HIV/AIDS and neuro-AIDS, including a history of the disease, and an explanation of many of the conditions that can arise in afflicted patients, including opportunistic infections, central nervous system tumors, spinal cord disorders, myopathies and progressive encephalopathy, amongst others. Clinicians will gain a greater understanding of the complex mechanisms of the disease. Beginning with a basic introduction to HIV infections and Neuro-AIDS, practitioners will find useful data on advances in molecular biology, neuroepidemiology, neuroimaging, neuropathology, neuropharmacology, as well as information on the development of therapeutic strategies appropriate for the disorder, including groundbreaking retroviral therapies. In addition, the socioeconomic and political constraints that hinder treatment and disease management in developing parts of the world are presented. * A comprehensive understanding of HIV/AIDS and neuro-AIDS, and the progression of the scientific community's understanding of the disease * Detailed information on fields such as neuroepidemiology, neuropathology, neuropharmacology, and neuroimaging and their contributions to HIV/AIDS research * Subject specific chapters on conditions associated with HIV/AIDS, including opportunistic infections, central nervous system tumors, and myopathies, amongst others
Author: Karin Moelling
Publisher: World Scientific
ISBN: 9811224765
Category : Science
Languages : en
Pages : 446
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Book Description
Coronavirus, AIDS, and Ebola: Viruses are normally defined as pathogens. Most viruses are, however, not enemies or killers. Well-known virologist and cancer researcher Karin Moelling describes surprising insights about a completely new and unexpected world of viruses. Viruses are ubiquitous, in the oceans, our environment, in animals, plants, bacteria, in our body, even in our genomes. They influence our weather, can contribute to control obesity, and can surprisingly be applied against threatening multi-resistant bacteria. The success story of the viruses started more than 3.5 billion years ago in the dawn of life when even cells did not exist. They are the superpower of life. There are more viruses on earth than stars in the sky. Viruses are everywhere. Some of them are incredibly ancient. Many viruses are hundredfold smaller than bacteria, but others are tenfold bigger and they were discovered only recently — the giant viruses, even deep within the permafrost where they were reactivated after 30,000 years.The author talks about a completely new world of viruses, which are based on the most recent, in part her own research results. Could viruses have been our oldest ancestors? Have viruses even 'invented' social behavior, do they lead to geniuses such as Mozart or Einstein — or alternatively to cancer? They can help to cure cancer. In this book, the author made a clear distinction between what is fact and what is her vision. This book is written for a general audience and not just for the experts. Its aim is to stimulate thinking, and perhaps to attract more young scientists to enter this field of research.This revised edition is brought up to date by a new chapter on the SARS-CoV-2 pandemic. Related Link(s)
Author: Loren K. Clarke
Publisher: Branden Books
ISBN: 9780828319188
Category : Health & Fitness
Languages : en
Pages : 356
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Book Description
The book begins with a detailed and technical journey into the laboratories where the keys to the understanding of the virus were forged in the early years of the search for the infectious agent of AIDS. The discovery of the virus, which was originally called HTLV-III/LAV and which became known as HIV, is only a small part of the saga.
Author: Dorland
Publisher: Elsevier Health Sciences
ISBN: 1455709859
Category : Medical
Languages : en
Pages : 2176
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Book Description
Thoroughly updated, this user-friendly reference, trusted for more than a century by healthcare personnel at every professional level, allows you to grasp the meanings of all medical terms in current usage. Understand and correctly use all the latest terminology in today's ever-evolving medical field with the 32nd Edition of the comprehensive, highly respected Dorlands Illustrated Medical Dictionary! - Enhance your understanding of all the current medical terminology in your field by relying on the most comprehensive and highly respected medical dictionary, bringing you more than 120,000 well-defined entries and 1500 clear illustrations. - Make sure you're familiar with the very latest medical terms used today with more than 5,500 new entries drawn from current sources. - Complement your understanding of new words and ideas in medicine with 500 new illustrations - Get more information in a smaller amount of space as the revised entry format includes related parts of speech.
Author: Elaine A. Moore
Publisher: McFarland
ISBN: 1476605211
Category : Health & Fitness
Languages : en
Pages : 289
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Book Description
More than 30 different organisms cause infection and disease when transmitted by venereal contact. Sexually transmitted diseases (STDs) represent 87 percent of all reported infections. This encyclopedia provides a wealth of information on STDs and associated topics, including care centers and departments, legal issues, and related associations and organizations. Coverage of the diseases includes such information as history, causes and origins; risk factors; diagnostic tests; considerations in pregnancy; and much more. The work concludes with a wide selection of resources. Books, journals, support groups, hotlines, organizations, and Internet sites are listed, along with sources for information on topics such as low cost prescription drug programs, social security disability, health insurance, and clinical trials.
Author: Matthew Hupert
Publisher: Mitten Press
ISBN: 9780984070084
Category : Poetry
Languages : en
Pages : 0
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Book Description
Hupert's rock-solid collection of 87 poems examine life, lust, love, religion, and politics in a word-play phantasmagoria in which, according to poet Dean Kostos, "sound is meaning and syntax is thrummed like strings on a guitar."
Author: Masanao Miwa
Publisher: VSP
ISBN: 9789067640572
Category : Science
Languages : en
Pages : 358
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Book Description
In the Proceedings of this Symposium, papers are presented from leading laboratories worldwide studying human and animal retroviruses and their associated leukemias and other diseases, including AIDS. The volume provides an up-to-date review of the field and indicates possible future developments for cancer research in which multidisciplinary work, ranging from molecular biology to epidemiology, plays an important role. The book contains 31 papers which are grouped into the following subject areas: lectures; clinical aspects; epidemiology; virus transmission; characterization of viruses; infected cells; AIDS.
Author: Paul Racz
Publisher: Karger Medical and Scientific Publishers
ISBN: 3318042986
Category : Medical
Languages : en
Pages : 216
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Book Description
Author: P.K. Vogt
Publisher: Springer Science & Business Media
ISBN: 3642701132
Category : Medical
Languages : en
Pages : 266
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Book Description
characteristic features in common with the genome of other retroviruses: long terminal repeats (L TR), and coding regions for internal proteins (gag), for re verse transcriptase (pol), and for glycosylated virion surface proteins (env) , ar ranged in the sequence gag, pol, env from the 5' to the 3' end of the genome. However, the HTL V genome also contains some specific features not shared with all other retroviruses: the LTR regions are unusually long (745 base pairs, with 298 base pairs constituting the R region), but unlike the long L TRs of mouse mammary tumor viruses, they do not contain open reading frames. A stretch of noncoding sequences separates the gag and the pol genes. Most interestingly, the HTLV genome contains a region between the 3' end of the env gene and the L TR, called the pX region, that encompasses four open reading frames. Leukemic T cells freshly obtained from patients contain the HTL V provirus but usually do not express it. However, once established in culture, these cells produce viral proteins and release type C particles. Likewise, T cells infected and transformed by HTL V in vitro synthesize virus. Such producing cell lines have been widely used in seroepidemiological surveys and continue to be of importance for detailed studies of viral proteins and nucleic acids.
