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Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 9780824779030
Category : Science
Languages : en
Pages : 438
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Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.
Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 9780824779030
Category : Science
Languages : en
Pages : 438
Get Book Here
Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.
Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 1482231670
Category : Science
Languages : en
Pages : 417
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Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behaviour and how to determime appropriate operating conditions, this reference illustrates procedures, apparatus and types of ion exchanges, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, this text explains the effects of each variable on separation behaviour; compares models and equations describing behaviour of proteins in IEC with experimental results; introduces design calculation procedures for scale up, adapting IEC for individual requirements; discusses parameters affecting separation behaviour, including adsorption equilibria, stationary phase diffusion and axial dispersion; provides examples of applications, such as high or medium performance IEC and large scale operations and reviews both theorectical and experimental literature. This text serves as an reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists and biotechnologists.
Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 9780367451318
Category :
Languages : en
Pages : 401
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Book Description
This book describes the separation mechanism of proteins in ion-exchange chromatography (IEC) by stressing the unique characteristics of IEC of proteins. It helps reader to acquire or improve the technique of IEC of proteins without calling for a deep understanding of the mathematics.
Author: Dermot Walls
Publisher: Humana Press
ISBN: 9781607619147
Category : Science
Languages : en
Pages : 527
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Book Description
A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Author: Lorette C. Javois
Publisher: Springer Science & Business Media
ISBN: 1592592139
Category : Science
Languages : en
Pages : 928
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Book Description
Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
Author: Jan-Christer Janson
Publisher: John Wiley & Sons
ISBN: 9780471186267
Category : Medical
Languages : en
Pages : 712
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Book Description
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Author: Arne Staby
Publisher: John Wiley & Sons
ISBN: 111903115X
Category : Technology & Engineering
Languages : en
Pages : 608
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Book Description
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Author: Alfred Nisonoff
Publisher: Academic Press
ISBN: 1483273857
Category : Nature
Languages : en
Pages : 560
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Book Description
The Antibody Molecule reviews the literature leading to current knowledge of the structure of immunoglobulins. The book begins by outlining some of the basic structural characteristics of immunoglobulins without citing the references on which the information is based. Separate chapters follow covering the chemical nature of the active site of an antibody molecule and mechanisms of interaction with hapten; the general structural features and properties of the various classes of human immunoglobulin; and amino acid sequences of human and mouse L chains and of human and rabbit H chains. Subsequent chapters deal with the evolution of the immunoglobulin classes; special properties of mouse, guinea pig, rabbit, and horse immunoglobulins; idiotypic specificities of immunglobulins; and the genetic control of antibodies. This book is meant for immunologists who have not personally observed the development of this exciting period in the history of immunology. It will also provide useful supplemental reading for the serious student or investigator who wishes to become familiar with the nature of the antibody molecule, its genetic control, and mode of action.
Author: Inamuddin
Publisher: Springer
ISBN: 3030060829
Category : Medical
Languages : en
Pages : 232
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Book Description
This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists.
Author: Kakoli Bose
Publisher: Springer Nature
ISBN: 9811649871
Category : Medical
Languages : en
Pages : 315
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Book Description
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
