Investigation of Biochemical Transformations in the Enhanced Biological Phosphorus Removal Process in Laboratory-scale Reactors and a Full-scale Wastewater Treatment Plant PDF Download
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Author: Jens Kruse
Publisher:
ISBN:
Category :
Languages : en
Pages : 104
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Author: Jens Kruse
Publisher:
ISBN:
Category :
Languages : en
Pages : 104
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Author: Rogelio Ernesto Zuniga Montanez
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Municipal wastewater contains a number of constituents that can have detrimental effects if discharged to receiving water bodies. Phosphorus (P) is of specific interest as a limiting nutrient in aquatic ecosystems that can cause eutrophication. In enhanced biological phosphorus removal (EBPR), polyphosphate accumulating organisms (PAOs) store excess P intracellularly. To achieve this accumulation, the organisms are exposed consecutively to anaerobic and either aerobic or anoxic conditions. During the anaerobic phase, PAOs consume and store organic carbon with P release, followed by the aerobic/anoxic phase during which the stored carbon is oxidized and P is taken up and stored as polyphosphate. PAOs are not the only bacteria that can thrive under these cyclic conditions and they face competition from glycogen accumulating organisms (GAOs). The latter have a similar metabolism but do not accumulate P. Most research to date has focused on the use of certain volatile fatty acids (VFAs) as carbon sources and on process conditions at temperatures common in temperate climates. Much remains unknown about the potential of EBPR in tropical regions and the suitability of other carbon substrates to drive the accumulation of phosphate. The purpose of this dissertation is to contribute to the understanding of EBPR at high temperatures and with unconventional carbon sources. Three different studies were designed and conducted with the following aims: (a) to evaluate the long-term EBPR stability and key microbial community in a wastewater treatment plant (WWTP) designed to achieve P removal in Singapore, (b) to study the process efficiency, biochemical transformations and organisms involved in a laboratory-scale EBPR reactor fed by alternating the substrates acetate and glutamate, and (c) to assess the potential of using unconventional carbon sources for EBPR by testing glutamate and glucose as alternating substrates at the laboratory-scale. The research included experiments at the full- and laboratory-scale, all at a mean temperature of 30 °C. Sustained observations in all three studies served to uncover the biochemical and microbial community dynamics. In the full-scale study, I conducted a yearlong evaluation of the EBPR activity at a WWTP that had been retrofitted to facilitate EBPR in Singapore. A mean P removal efficiency of 90 % was observed throughout the sampling period, similar to temperate climate installations and contrary to earlier reports that EBPR was not feasible at high temperatures. The main PAOs present in the reactor were Tetrasphaera, Candidatus Accumulibacter (Accumulibacter) and Dechloromonas, with mean relative abundances of 1.53, 0.43 and 0.69 %, respectively. The PAO community underwent changes during the surveyed period, with a marked transition from a Tetrasphaera-dominated community to a more even one. The link between PAOs and the P released in the anaerobic compartment was supported by a statistically significant correlation between the relative abundance of these organisms and the measured P concentrations. GAOs and PAOs coexisted without compromising the EBPR activity. In one of the laboratory-scale studies, glutamate and acetate were alternated as the carbon source for a reactor operated at 30 °C. Complete and stable P removal was achieved with a predominantly glutamate-containing feed, after modifying operating parameters commonly used in VFA-fed systems to a COD/P ratio of 40:1 mg COD/mg P and a cycle duration of 8 h. Long-term EBPR with a feed dominated by glutamate in a laboratory-scale reactor has not been previously reported. The P and carbon cycling patterns were different for glutamate and acetate. Reduced P release and polyhydroxyalkanoate (PHA) accumulation happened when glutamate was fed, but not with acetate, where glutamate appeared to be stored as an unidentified non-PHA compound or as different compounds. The PAO Accumulibacter and the GAO Candidatus Competibacter (Competibacter) remained the only known EBPR bacteria during the period of good EBPR performance, at similar relative abundances. A canonical correlation analysis revealed that the relative abundance of some non-PAO organisms correlated more strongly with variables that denoted good EBPR activity than did the abundance of any of the known PAOs. In the last study, a laboratory-scale sequencing batch reactor was used to test the EBPR potential of glutamate and glucose as alternating carbon sources in a high temperature process. The recommended influent COD/P ratio and batch duration for VFA-fed systems were unsuccessful. After modifications, COD/P ratios of 20:1 and 40:1 mg COD/ mg P resulted in complete P removal, but only in the short term. The EBPR stoichiometry with these two carbon substrates differed from that of VFA-fed systems. For both, lower P and PHA cycling was observed, and intracellular carbon storage compounds that were not PHA appeared to contribute to P cycling, as shown from carbon balances. A very diverse EBPR community was present in the reactor, including Accumulibacter, Tetrasphaera and Dechloromonas PAOs, and Competibacter, Defluviicoccus, Micropruina and Kineosphaera GAOs. Most of these organisms have not been reported before in laboratory-scale EBPR reactors operated at high temperatures. The work presented in this dissertation expands the understanding of EBPR by showing that the process is possible and stable in full-scale treatment plants at high temperature, with removal efficiencies similar to those observed in temperate climates. In addition, it was shown that unconventional carbon sources, specifically, glutamate and glucose, do participate in EBPR and that complete and stable phosphorus removal can be achieved with glutamate as dominant substrate at high temperature. A core PAO and GAO community was present in the three systems, where the interactions among members were more complex than previously considered, including competition, coexistence and succession events. The results obtained from this work enhance our fundamental knowledge of EBPR as an industrial process, as well as the metabolic diversity, niches and dynamics of PAOs and GAOs. The study outcomes can inform design and operational strategies at full-scale treatment plants. Lastly, the consideration of both high temperatures and unconventional carbon sources for EBPR is expected to aid in the development of more efficient treatment processes.
Author: Cao Ye Shi
Publisher: IWA Publishing
ISBN: 1843393816
Category : Science
Languages : en
Pages : 151
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Special Offer: Cao Ye Shi Author Set - Buy all three books together and save a total £76! Biological Phosphorus Removal Activated Sludge Process in Warm Climates presents the results of detailed research on the Enhanced Biological Phosphorus Removal (EBPR) activated sludge process under warm climate conditions (20oC - 30oC), which is part of the R & D program of Public Utilities Board (PUB) Singapore. The investigations and studies presented in this book are application-oriented, but at the same time the studies aim at an insightful understanding of the EBPR with the knowledge of the latest development in academic field. The focus points are: EBPR performance of laboratory-scale and full-scale activated sludge processes under the site conditions in warm climates The carbon competition and distribution between PAO and GAO (and denitrifiers) in the process The stoichiometry and kinetics of P-release, COD uptake in the anaerobic environment and P-uptake in the aerobic environment under different temperatures and operating conditions PAO and GAO population fractions, shift and dominance studies using FISH and batch tests The inter-relationships between the system performance, process design and the microbial community EBPR for industrial wastewater (high ratio of feed COD/P) treatment under warm climates. Together with the preceding book – Biological Nitrogen Removal Activated Sludge Process in Warm Climates – published by IWA in 2008, this book fills the gap of biological nutrient (nitrogen and phosphorus) removal in warm climates and provides unique experiences and knowledge for Process and design researchers and engineers in wastewater research, students and academic staff in Civil/Sanitation/Environment Departments, as well as Managers, Engineers and Consultants in water companies and water utilities. Visit the IWA WaterWiki to read and share material related to this title: http://www.iwawaterwiki.org/xwiki/bin/view/Articles/SELECTIONOFDOMESTICWASTEWATERTREATMENTSYSTEMSINWARMCLIMATEREGIONS
Author: P. M. J. Janssen
Publisher: IWA Publishing
ISBN: 9781843390121
Category : Science
Languages : en
Pages : 228
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Biological phosphorus (bio-P) removal has become a reliable and well-understood process within wastewater treatment, despite being one of the most complex processes in the activated sludge process. Extended fundamental and full-scale research has been carried out into the bio-P process and the state-of-the-art is described in this report. A summarising historical overview gives insight into the establishment of the appropriate microbiological and biochemical basis of the process and the development of bio-P configurations in practice. Aspects of the bio-P process that have a direct influence on the efficiency of phosphorus removal are subjected to an in-depth investigation. This report presents guidelines for design and dimensioning in order to introduce and/or optimise the bio-P process in practice. Twelve bio-P installations are extensively described and the operational results and experiences are related to existing bio-P knowledge and guidelines. Based on a number of parameters, a comparison is made between the described bio-P plants. A steady state model is verified with extensive periods of practical experience of the plants. The bio-P model, which is provided on CD-ROM (available for download here), offers a reliable insight into the bio-P process, coupled with sensitivity analyses regarding wastewater characteristics and process parameters for the anaerobic volume and the P-ortho concentration in the final effluent. The report ends with a systematic approach to the design of the bio-P process, based on the background of the bio-P process itself, much practical experience and the analysis of operational bio-P plants. Also presented is a systematic approach to tackle operational aspects of the bio-P process in order to generate an acceptable low P effluent concentration. This optimisation of the bio-P process operation is supported by a decision diagram. Biological Phosphorus Removal will be an invaluable source of information for all those concerned with wastewater treatment, including plant managers, process designers, consultants and researchers.
Author: Yuqi Wang
Publisher:
ISBN:
Category : Phosphorus
Languages : en
Pages : 76
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Book Description
Phosphorus is an essential element for every living organism, but when it exceeds certain limit in water bodies, it will cause serious environmental issues, such as eutrophication. P accumulation in water bodies can be caused by non-point sources, such as agriculture land runoff, as well as from point sources such as wastewater effluent discharges. In order to avoid eutrophication of water bodies the regulatory agency have traditionally pushed for the removal of phosphorus at wastewater treatment plants rather than regulating the non-point sources; this has been done imposing strict limits on wastewater effluents to natural watershed. At the same time, phosphorus stock on the earth is a limited resource, and its quantity is decreasing steadily, due to its use in the fertilizers production. In order to support the population growth on the earth, phosphorus recovery, especially from wastewater streams has to be considered. Biological processes that remove phosphorus from wastewater, are called Enhanced Biological Phosphorus Removal (EBPR in short) processes, and produce a P rich sludge that can be treated to recover phosphorus in forms that could be used as fertilizers. Several processes have been developed to recover P from EBPR plants, such as Waste Activated Sludge Stripping to Remove Internal Phosphorus (WASSTRIP) and the PhoStrip processes. P recovery within EBPR plant often involved an anaerobic holding tank, where P is released due to PAO activity as well as bacteria decay. However, the impact of EBPR process operating conditions on P-release capacity and kinetics are not fully understood. In addition, it is largely unclear how the anaerobic digestion process of the P-recovery process affects the microbial population, and therefore the EBPR activity in the mainstream, in system where the sludge is recirculated back to the mainstream. In this study, P-release capacity and kinetics were studied by conducting day long endogenous anaerobic digestion tests on activated sludge withdrawn from lab scale sequencing batch reactors operating under different conditions (COD/P and SRT). P-release mechanisms during the digestion test were investigated by Live/Dead analysis, as well as soluble metal ion concentration measurements, which are usually associated with EBPR activity. In addition, PAO activities changes were explored by the microbial population quantification, combined with P-release rate in the present of VFA. Considering the microbial populations, in the acetate fed SBRs are different in quantity and possibly composition, from the population (especially PAOs) in full-scale samples, the same anaerobic test and measurements were performed on a full-scale EBPR WWTP WAS samples. Under anaerobic starvation conditions, it was observed that low COD:P ratio with 10-20 days-SRT had better P recovery potential than other operation conditions involved in this study, in terms of higher amount ortho-P released and faster releasing rate. Among the released ortho-P during the digestion test, majority of it was found to be due by poly-P depletion. In addition, because of the reducing intercellular polymer storage, PAO activity also decreased significantly during the anaerobic digestion test. However, with different population and composition, PAO activity in full scale WAS samples actually increased after the test.
Author: R. Ramadori
Publisher: Elsevier
ISBN: 1483160599
Category : Technology & Engineering
Languages : en
Pages : 405
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Book Description
Biological Phosphate Removal from Wastewaters contains the proceedings of an International Association on Water Pollution Research and Control Specialized Conference held in Rome, Italy on September 28-30, 1987. Contributors review advances that have been made in the removal of biological phosphates from wastewaters, both at the fundamental scientific level and in the practical application of the process. Topics range from the fundamental microbiology and biochemistry of the enhanced biological removal of phosphate to the practical full-scale plant experiences with phosphorus removal and sludge handling from such processes. This text is comprised of 43 chapters; the first of which describes the utilization of polyphosphate as an energy reserve in Acinetobacter sp. and activated sludge. Attention then turns to metabolic control in polyphosphate-accumulating bacteria and its role in enhancing biological phosphate removal. The biochemistry and energetics of biological phosphorus removal are also considered. The next section is devoted to process modeling and includes chapters that explore the kinetics of biological excess phosphorus removal; factors affecting anaerobic stabilization during biological phosphorus removal; and the behavior of magnesium in biological phosphate removal. In the next section, bench/pilot-scale studies are presented; one of which investigated the reduction of returned phosphorus from a sludge treatment process. The book concludes with a discussion on phosphate removal mechanisms and pilot- and full-scale experiences. This book will be of interest to students, practitioners, and policymakers in water pollution control.
Author: Chang Hoon Ahn
Publisher:
ISBN:
Category :
Languages : en
Pages : 302
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Author: S. Bathe
Publisher: IWA Publishing
ISBN: 9781843395096
Category : Science
Languages : en
Pages : 186
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Aerobic Granular Sludge has recently received growing attention by researchers and technology developers, worldwide. Laboratory studies and preliminary field tests led to the conclusion that granular activated sludge can be readily established and profitably used in activated sludge plants, provided 'correct' process conditions are chosen. But what makes process conditions 'correct'? And what makes granules different from activated sludge flocs? Answers to these question are offered in Aerobic Granular Sludge. Major topics covered in this book include: Reasons and mechanism of aerobic granule formation Structure of the microbial population of aerobic granules Role, composition and physical properties of EPS Diffuse limitation and microbial activity within granules Physio-chemical characteristics Operation and application of granule reactors Scale-up aspects of granular sludge reactors, and case studies Aerobic Granular Sludge provides up-to-date information about a rapidly emerging new technology of biological treatment.
Author: D. Dochain
Publisher: IWA Publishing
ISBN: 9781900222501
Category : Science
Languages : en
Pages : 360
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Book Description
Environmental quality is becoming an increasing concern in our society. In that context, waste and wastewater treatment, and more specifically biological wastewater treatment processes play an important role. In this book, we concentrate on the mathematical modelling of these processes. The main purpose is to provide the increasing number of professionals who are using models to design, optimise and control wastewater treatment processes with the necessary background for their activities of model building, selection and calibration. The book deals specifically with dynamic models because they allow us to describe the behaviour of treatment plants under the highly dynamic conditions that we want them to operate (e.g. Sequencing Batch Reactors) or we have to operate them (e.g. storm conditions, spills). Further extension is provided to new reactor systems for which partial differential equation descriptions are necessary to account for their distributed parameter nature (e.g. settlers, fixed bed reactors). The model building exercise is introduced as a step-wise activity that, in this book, starts from mass balancing principles. In many cases, different hypotheses and their corresponding models can be proposed for a particular process. It is therefore essential to be able to select from these candidate models in an objective manner. To this end, structure characterisation methods are introduced. Important sections of the book deal with the collection of high quality data using optimal experimental design, parameter estimation techniques for calibration and the on-line use of models in state and parameter estimators. Contents Dynamical Modelling Dynamical Mass Balance Model Building and Analysis Structure Characterisation (SC) Structural Identifiability Practical Identifiability and Optimal Experiment Design for Parameter Estimation (OED/PE) Estimation of Model Parameters Recursive State and Parameter Estimation Glossary Nomenclature
Author: R. F. Drnevich
Publisher:
ISBN:
Category : Phosphorus
Languages : en
Pages : 156
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