Investigating the Micro-vortex Effects on Microfluidic Label-free Techniques for Circulating Tumor Cell Separation PDF Download
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Author: Arian Aghilinejad
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Arian Aghilinejad
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Takasi Nisisako
Publisher: MDPI
ISBN: 3039366947
Category : Technology & Engineering
Languages : en
Pages : 230
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Book Description
Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.
Author: James Che
Publisher:
ISBN:
Category :
Languages : en
Pages : 76
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Book Description
Disseminated and circulating tumor cells are key players of metastatic cancer and have high diagnostic and prognostic value. These cells may often accumulate and persist in body fluids--such as in pleural effusions or blood--which are minimally-invasive sources for patient sampling. However, tumor cells are relatively scarce and must typically be enriched from a large background of blood cells. An inertial microfluidic device was developed to specifically trap and concentrate large tumor cells in stable microvortices which form under high flow rates. The novel label-free Vortex platform was optimized to isolate large rare cells at high efficiency (up to 80%), purity (10-80%), concentration (~200 L volume), and viability (>80%) in a short time period (
Author: Christopher Michael Landry
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages :
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Book Description
Throughout the world, cancer is a primary health concern due its high mortality rate. The typical cause of death from cancer is metastasis, which is the spreading of a primary tumor to distant organs. Currently, cancer metastasis is attributed to Circulating Tumor Cells (CTCs). A CTC is a cancer cell that has dislodged from the primary tumor and entered the blood stream. In order to achieve early cancer detection and improve patient prognosis, CTCs must be separated from whole blood samples. One of the most promising ways to achieve this separation is through microfluidic devices. Unfortunately, experimental testing of microfluidic devices is expensive, time-consuming, and lacks the ability to demonstrate underlying physics. To help resolve the issues associated with experimental testing, numerical modeling is employed. Here, two types of label-free microfluidic devices are modeled and tested. First, a microfiltration device is modeled and the effects of a non-axisymmetric approach are tested. From the results, critical pressure was found to be a robust design criterion for microfiltration devices regardless of CTC approach condition. CTC transit time on the other hand was determined to have a dependence on approach condition; therefore, should not be used in designing microfiltration devices. The other type of label-free microfiltration device tested was a Deterministic Lateral Displacement (DLD) device. Here, underlying causes of experimental observations for a symmetric airfoil shaped pillar design were achieved through numerical modeling of flow fields and array anisotropy. Results show that array anisotropy is responsible for creating a lateral shift in the flow field. Critical size of the DLD device is reduced when the flow field shifts toward the direction of bumped motion, and increases when shifting occurs away from bumped motion. Additionally, an equation is proposed that relates migration angle to anisotropy via pseudoperiodicity. Lastly, a working limit for symmetric airfoil shaped pillar DLD devices was found to be between -25° and -35° angle of attack. These findings will aid in future design work and open the possibility of new applications for micro fabricated DLD devices by achieving smaller critical sizes than previously possible.
Author: Jose L. Garcia-Cordero
Publisher: Springer Nature
ISBN: 107163271X
Category : Medical
Languages : en
Pages : 327
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Book Description
This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.
Author: David Caballero
Publisher: Springer Nature
ISBN: 3031040392
Category : Medical
Languages : en
Pages : 599
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Book Description
This book offers a comprehensive overview of the development and application of microfluidics and biosensors in cancer research, in particular, their applications in cancer modeling and theranostics. Over the last decades, considerable effort has been made to develop new technologies to improve the diagnosis and treatment of cancer. Microfluidics has proven to be a powerful tool for manipulating biological fluids with high precision and efficiency and has already been adopted by the pharmaceutical and biotechnology industries. With recent technological advances, particularly biosensors, microfluidic devices have increased their usefulness and importance in oncology and cancer research. The aim of this book is to bring together in a single volume all the knowledge and expertise required for the development and application of microfluidic systems and biosensors in cancer modeling and theranostics. It begins with a detailed introduction to the fundamental aspects of tumor biology, cancer biomarkers, biosensors and microfluidics. With this knowledge in mind, the following sections highlight important advances in developing and applying biosensors and microfluidic devices in cancer research at universities and in the industry. Strategies for identifying and evaluating potent disease biomarkers and developing biosensors and microfluidic devices for their detection are discussed in detail. Finally, the transfer of these technologies into the clinical environment for the diagnosis and treatment of cancer patients will be highlighted. By combining the recent advances made in the development and application of microfluidics and biosensors in cancer research in academia and clinics, this book will be useful literature for readers from a variety of backgrounds. It offers new visions of how this technology can influence daily life in hospitals and companies, improving research methodologies and the prognosis of cancer patients.
Author: Hashem Mohammad Abul
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages :
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Book Description
Circulating Tumor Cells (CTCs) are potential indicators of cancer. Detection of CTCs is important for diagnosing cancer at an early stage and predicting the effectiveness of cancer treatment. Recent progress in the development of microfluidic chips has inaugurated a new possibility for designing diagnostic devices for early cancer detection. Among various devices, deformation-based CTC microchips have shown a strong promise for CTC detection due to its simplicity and low cost. This type of devices involves a process where CTCs are trapped while allowing more deformable blood cells to squeeze through the filtration geometry at the specified operating pressure. Fundamental understanding of CTC passing event through a micro-filtering channel seems to be a promising direction in studying these microdevicessince it helps optimize the microfilter design for achieving high isolation purity and capture efficiency. Along with the experimental studies, numerical simulation emerges as a powerful tool to predict the behavior of a cell inside a microfilter, and may deliver important insights to optimize the processes by saving time and cost. First, the CTC squeezing process through a microfluidic filtering channel is studied by modeling the CTC as a simple liquid droplet. Cell modeling employed both Newtonian and non-Newtonian approaches to simplify the model and investigating different biophysical properties. Detailed microscopic multiphase flow characteristics regarding the filtering process are discussed including the pressure signatures, flow details, and cell deformation. Next, we employed a compound droplet model consisting of an outer cell membrane, cytoplasm and the nucleus to study the flow dynamics more realistically. The effects of different parameters such as the nuclear to cytoplasmic size ratio (N/C), operating flow rate and viscosity of the cell has been investigated. We studied critical pressure for the CTC at different flow rates as it plays a crucial role in the device operation in ensuring a successful passing event. Our study provides an insight into the cell squeezing process and its characteristics, which can guide in the design and optimization of next-generation deformation-based CTC microfilters.
Author: Takasi Nisisako
Publisher: Mdpi AG
ISBN: 9783036536743
Category : Technology & Engineering
Languages : en
Pages : 112
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Book Description
Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the-art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.
Author: Takasi Nisisako
Publisher:
ISBN: 9783039366958
Category :
Languages : en
Pages : 230
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Book Description
Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.
Author: Wujun Zhao
Publisher:
ISBN:
Category :
Languages : en
Pages : 332
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Book Description
Microfluidic cell separation based on negative magnetophoresis in ferrofluids (ferrohydrodynamics) has unique advantages over other competing techniques. Magnetic force does not interact directly with cells, minimizing potential detrimental effects on them. Systems based on negative magnetophoresis are simple and low-cost, only requiring microchannels and permanent magnets or electromagnetic coils. As a result, negative magnetophoresis has been used to manipulate particles and cells. Negative magnetophoresis also eliminates the labeling steps through the incorporation of a special medium into the assay. This medium, typically magnetic liquids such as a paramagnetic salt solution or a ferrofluid, possesses a larger magnetization than the cells. An external magnetic field attracts the magnetic medium, which causes the cells to be preferentially pushed away. Consequently, cells can be manipulated magnetically without the need for labeling them. A water-based biocompatible ferrofluid that not only maintains its colloidal stability under strong magnetic fields but also keeps cells alive was developed for cell separation. Ferrohydrodynamic cell separation in this biocompatible ferrofluids offered moderate throughput (~106 cells h-1 in this study) and extremely high separation efficiency (>99%) for HeLa and blood cells without the use of labels. A microfluidic device was further designed and optimized specifically to shorten the time of live cells' exposure to ferrofluids from hours to seconds, by eliminating time-consuming off-chip sample preparation and extraction steps and integrating them on-chip to achieve a one-step process. As a proof-of-concept demonstration, a ferrofluid with 0.26% volume fraction was used in this microfluidic device to separate spiked cancer cells from cell lines at a concentration of 8́ơ100 cells per mL from white blood cells with a throughput of 1.2 mL h-1. The average separation efficiency was 82.2% and the separated cancer cells' purity was between 25.3%-28.8%. Later, we demonstrated the development of a laminar-flow microfluidic device that was capable of enriching rare circulating tumor cells from patients' blood in a biocompatible manner with a high throughput (6 mL h-1) and a high rate of recovery (92.9%). Biocompatibility study on lung and breast cancer cell lines showed that separated cancer cells had excellent short-term viability, normal proliferation and unaffected key biomarker expressions.
