In Vivo and in Vitro Studies with Growing Pigs on Standardised Ileal Amino Acid Digestibilities in Grain Legumes PDF Download
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Author: Dagmar Jezierny
Publisher: Cuvillier Verlag
ISBN: 3736931786
Category : Science
Languages : en
Pages : 156
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Book Description
Since there is a general ban on meat and bone meal and its by-products in diets for livestock in the EU, it is crucial to focus on alternative protein feed ingredients to be used in pig nutrition. Beside soybean meal as a commonly used plant protein supplement, grain legumes may also be used as alternative protein sources in diets for pigs. Current protein evaluation system for feed ingredients for pigs are based on the concept of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA), but there is a scarcity of information on SID of CP and AA in grain legumes grown in Central Europe. Therefore, the objective of the thesis was to determine the chemical composition and the SID of CP and AA in seed-grade cultivars of faba beans (Vicia faba), peas (Pisum sativum) and lupins (Lupinus spp.) in growing pigs using the difference method. Furthermore, to obtain SID of CP and AA, the basal ileal endogenous CP and AA losses in growing pigs were estimated by means of regression analysis from apparent ileal digestible and total dietary contents of CP and AA. A further aim of the thesis was directed to the validation of an in vitro procedure for estimates of SID of CP and AA in the same grain legumes by means of a two-step enzymatic method with subsequent pepsin and pancreatin incubations. The in vitro predicted SID values were compared with their corresponding in vivo SID values. The chemical composition and SID values of CP and AA measured in faba bean, pea and lupin cultivars generally were in good agreement with tabulated values. The average SID of CP and most AA in lupin cultivars (CP, 87%) were higher than in pea (CP, 79%) or faba bean cultivars (CP, 76%) Comparably low SID values in some faba bean cultivars can be partly explained by their contents of condensed tannins. In contrast to SID values in faba bean cultivars, there were only small variations in SID values between individual cultivars of peas or lupins. Results of the in vitro experiment revealed that predicted SID of CP and AA do not provide direct estimates of SID values in grain legumes, because the in vitro predicted SID values were generally higher than in vivo SID values. The use of multiple linear regression analysis with grain legume species as a covariate resulted in strong linear relationships. Further investigations are warranted to study if these principles apply to other feed ingredients as well. Moreover, the use of in vitro predicted SID values in practical diet formulations for pigs needs to be further validated.
Author: Dagmar Jezierny
Publisher: Cuvillier Verlag
ISBN: 3736931786
Category : Science
Languages : en
Pages : 156
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Book Description
Since there is a general ban on meat and bone meal and its by-products in diets for livestock in the EU, it is crucial to focus on alternative protein feed ingredients to be used in pig nutrition. Beside soybean meal as a commonly used plant protein supplement, grain legumes may also be used as alternative protein sources in diets for pigs. Current protein evaluation system for feed ingredients for pigs are based on the concept of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA), but there is a scarcity of information on SID of CP and AA in grain legumes grown in Central Europe. Therefore, the objective of the thesis was to determine the chemical composition and the SID of CP and AA in seed-grade cultivars of faba beans (Vicia faba), peas (Pisum sativum) and lupins (Lupinus spp.) in growing pigs using the difference method. Furthermore, to obtain SID of CP and AA, the basal ileal endogenous CP and AA losses in growing pigs were estimated by means of regression analysis from apparent ileal digestible and total dietary contents of CP and AA. A further aim of the thesis was directed to the validation of an in vitro procedure for estimates of SID of CP and AA in the same grain legumes by means of a two-step enzymatic method with subsequent pepsin and pancreatin incubations. The in vitro predicted SID values were compared with their corresponding in vivo SID values. The chemical composition and SID values of CP and AA measured in faba bean, pea and lupin cultivars generally were in good agreement with tabulated values. The average SID of CP and most AA in lupin cultivars (CP, 87%) were higher than in pea (CP, 79%) or faba bean cultivars (CP, 76%) Comparably low SID values in some faba bean cultivars can be partly explained by their contents of condensed tannins. In contrast to SID values in faba bean cultivars, there were only small variations in SID values between individual cultivars of peas or lupins. Results of the in vitro experiment revealed that predicted SID of CP and AA do not provide direct estimates of SID values in grain legumes, because the in vitro predicted SID values were generally higher than in vivo SID values. The use of multiple linear regression analysis with grain legume species as a covariate resulted in strong linear relationships. Further investigations are warranted to study if these principles apply to other feed ingredients as well. Moreover, the use of in vitro predicted SID values in practical diet formulations for pigs needs to be further validated.
Author:
Publisher: ScholarlyEditions
ISBN: 1464965099
Category : Science
Languages : en
Pages : 1747
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Book Description
Issues in Animal Science and Research / 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Animal Science and Research. The editors have built Issues in Animal Science and Research: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Animal Science and Research in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Animal Science and Research: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Pia Rosenfelder
Publisher: Cuvillier Verlag
ISBN: 3736949464
Category : Science
Languages : en
Pages : 154
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Book Description
Wheat is the primarily cereal grain produced within the European Union. Due to its high starch content it is the dominating energy feed ingredient in diets for pigs. However, due to its high dietary inclusion level wheat can also supply considerable high amounts of crude protein (CP) and amino acids (AA) to the animal. Wheat co-products of bioethanol production are a valuable source of CP and AA in animal nutrition. Therefore, the objectives of the present thesis were to study the nutritive value of eight recently introduced wheat genotypes and of one liquid wheat co-product of bioethanol production for growing pigs. For this purpose a comprehensive analysis of nutrients in the assay feedstuffs was conducted and standardized ileal digestibility (SID) of CP and AA in growing pigs was determined. There were significant differences in SID of CP and AA between the eight genotypes of wheat. However, digestibility values were lower when compared to literature data, leading to the assumption that data in actual feed tables might result in an overestimation of the protein value of wheat. The liquid wheat co-product is a valuable source of CP, AA and energy in diets for pigs. It is superior to dried bioethanol co-products as it is not exposed to excessive heat treatment during manufacturing resulting in greater digestibility values. In der Europäischen Union ist Weizen die am weitesten verbreitete Getreideart. Aufgrund seines hohen Stärkegehalts wird Weizen in Mastschweinerationen als Energiefuttermittel eingesetzt. Bei hohen Anteilen in der Gesamtration kann Weizen jedoch auch einen erheblichen Beitrag zur Deckung des Rohprotein- (XP) und Aminosäuren (AS)- Bedarfs des Tieres leisten. Nebenprodukte aus der Weizenverarbeitung bei der Bioethanolproduktion sind eine wertvolle Eiweißkomponente in der Tierernährung, dazu zählen Trocken- und Flüssigschlempen. Ziele dieser Arbeit waren die Bestimmung des Futterwerts von 8 aktuell verfügbaren Weizengenotypen sowie eines flüssigen Weizenschlempefutters für Mastschweine. Zu diesem Zweck wurde eine umfangreiche Nährstoffanalyse der Versuchsfuttermittel durchgeführt, sowie die standardisierte praecaecale Verdaulichkeit (pcVQ) von XP und AS bei Mastschweinen untersucht. Es gab signifikante Unterschiede der pcVQ von XP und AS zwischen den 8 Weizengenotypen. Allerdings waren die Verdaulichkeitswerte geringer als in der Literatur angeben, so dass Daten aus aktuellen Futterwerttabellen möglicherweise den Proteinwert von Weizen überschätzen. Das flüssige Weizenschlempefutter stellt eine wertvolle XP-, AS- und Energiequelle für Schweine dar. Es ist Trockenschlempen im Nährwert überlegen, da es keiner intensiven Hitzeeinwirkung ausgesetzt ist und somit höhere Verdaulichkeitswerte aufweist.
Author: Hanna Spindler
Publisher: Cuvillier Verlag
ISBN: 3736983158
Category : Science
Languages : en
Pages : 134
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Book Description
In 2013, more than 308 million tons of cereals were produced within the European Union, of which about 60 million tons were grown as barley. The protein content of barley is rather low when compared to protein feedstuffs such as soybean meal. However, due to its high dietary inclusion level, up to half of the protein requirement of pigs can be met by barley.
Author: M. F. Fuller
Publisher: Oxford University Press, USA
ISBN:
Category : Medical
Languages : en
Pages : 230
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Book Description
What are we trying to simulate?; Digestive enzyme activities in pigs and poultry; The role of the microflora in digestion; The physical and chemical constitution of foods: effects on carbohydrate digestion; Indirect effects of foods antinutrients on protein digestibility and nutritional value of diets; In vitro simulation of digestion and absorptionin the gastro-intestinal tract of pigs and poultry - methods and results; The chemical and biological bases of a calculation system developed for predicting dietary energy values: a poultry model; Enzyme modelling of protein digestion and L-lysine availability; Use of pepsin digentibility, multienzyme pH change and protein solubility assays to predict in vivo protein quality of feedstuffs; Estimation of true ileal digestibility of amino acids with pigs by an in vitro method using intestinal fluid; prediction of the energy value of non-ruminant feeds using in vitro digestion with intestinal fluid and other chemical methods; A model for feed evaluation based on in vitro digestible dry matter and protein; In vitro simulation of protein digestion: an integrated approach; Simultaneous estimations of precaecal protein and carbohydrate digestion in the pig; The way forward; the future requirements of the feed industry; The scientific challenge.
Author: Tobias Aumiller
Publisher: Cuvillier Verlag
ISBN: 3736980949
Category : Science
Languages : en
Pages : 164
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Book Description
Native carbohydrate fractions of major dietary components in pig nutrition, such as dietary fiber, resistant starch and oligosaccharides in whole cereal grains or grain legumes appear to be appropriate to manipulate the porcine gut microbiota. Therefore, the objective of this thesis was to evaluate effects of differences in carbohydrate composition of cereal grains, grain legumes and feed supplements on the intestinal microbiota in pigs. Based on a comprehensive literature research, an overview on the current research on the effect of whole grain cereals and grain legumes as well as their by-products on the pigs’ intestinal microbiota in relation to their carbohydrate composition was given. An in vivo experiment was carried out to determine the effects of 8 recently introduced genotypes of barley or wheat on ileal and fecal microbiota of grower pigs by means of quantitative real-time PCR. In addition, in a second experiment the effect of ultra-fine ground fibers from wheat, lupins and peas in comparison to inulin and basal substrates on the microbiota composition and formation of microbial metabolites was determined in vitro using the modified Hohenheim Gas Test. In agreement with previous studies, the potential to modulate the pigs’ intestinal microbiota with cereal grains and feed supplements based on cereal grain or grain legume fibers was shown. However, it was not possible to assign the observed effects on the bacterial groups to the dietary carbohydrates known to influence the intestinal microbiota, such as arabinoxylan or β-glucan.
Author: Elisa Strang
Publisher: Cuvillier Verlag
ISBN: 3736985460
Category : Science
Languages : en
Pages : 144
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Book Description
Cereal grains, such as rye and triticale are a major source of energy in pig nutrition. The crude protein (CP) content in cereal grains is rather low compared to protein ingredients such as soybean meal. However, costs and availability of soybean meal are strongly correlated with the price development of agricultural commodities on the world market. Therefore, swine producers will make increasing efforts to use protein from locally produced cereal grains more efficiently to reduce feed costs. Measurements of apparent (AID) and standardized ileal digestibility (SID) of CP and AA are frequently used to estimate the amounts of absorbable AA from the diet which are used for pigs’ metabolic processes. However, until now there is limited data on SID of CP and AA in rye and triticale, particularly in new genotypes. Therefore, one objective of the present work was to determine the variation in nutritional composition and SID of CP and AA of 8 currently available rye and 8 triticale genotypes. Standardized ileal digestibility of CP and most AA between the 8 assayed genotypes of both rye and triticale did not differ. Furthermore, average SID values of CP and AA of both rye and triticale in the present experiment were lower when compared to values of rye and triticale published in current feed tables. Thus, future research should clarify, whether these differences in comparison to feed tables are caused by experimental conditions or reflect a consistent decrease that need to be accounted for in feed tables.
Author: Arvind Kumar
Publisher: Springer Nature
ISBN: 3030932621
Category : Science
Languages : en
Pages : 325
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Book Description
This two-volume set discusses recent approaches and technological innovations for sustainable agriculture in smallholder farming systems impacted by climate change. The systems covered include crop-based agricultural production, as well as aquaculture and livestock production as related systems using similar techniques to combat food security issues brought about by climate change and resource overuse. The chapters detail innovations involving crop diversification, soil resilience management, geoinformatics and land suitability monitoring for smart farming, information technology in livestock production, and nutrient resource management in fishery aquaculture. Researchers, practitioners and industries will be able to use this information to implement socially and economically sustainable practices to achieve food security in impoverished areas vulnerable to climate change, while also learning about the rapid evolution in information technology that is applicable for and available to small holder farmers. Volume 2 focuses on trends and technologies in food security within the context of sustainable practices, drone technology, microwave data, molecular farming, machine learning, agricultural economics, spatial modeling and agricultural policy. These chapters discuss advancements in fishery resource and aquaculture practices, and also the challenges facing these areas due to climate change.
Author: Renata Urbaityte
Publisher: Cuvillier Verlag
ISBN: 3736930003
Category : Science
Languages : en
Pages : 142
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Book Description
A cautious assessment of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA) as a measure to describe AA bioavailabilities is critical for evaluating the protein value of feed ingredients for piglets. Estimates of SID have been generated for most feed ingredients for grower-finisher pigs, whereas corresponding values for piglets hardly exist. Until now, SID values determined in grower-finisher pigs are used in diet formulation for piglets as well. However, there is some concern that these values may not be valid in piglets due to a limited digestive capacity for feed protein in piglets. A guideline for the standardisation of the experimental procedure in digestibility experiments had already been proposed for grower-finisher pigs. These guidelines firstly include threshold levels for CP and AA in the assay diets to obtain so-called plateau apparent ileal digestibility values, which are independent of dietary CP and AA levels, and secondly values for IAALB. For piglets, similar guidelines, including dietary threshold levels and values for IAALB, have currently been established. Therefore, the objective of the thesis was, based on these guidelines, to determine SID of CP and AA in 24 assay feed ingredients, including 11 products from soybean processing, 7 by-products from starch processing, 2 fish meals and 4 whey proteins. For this purpose, a total of 4 digestibility experiments with piglets fitted with simple T-cannulas at the distal ileum was conducted. For each experiment, 14 barrows were weaned at 18 days of age. Each experiment consisted of 3 periods for digesta collection. A total of 24 semisynthetic cornstarch-based diets with various inclusion levels of casein were formulated to which 1 of the 24 assay feed ingredients each was added. The daily feed allowance was restricted to 30 g/kg of individual body weight throughout all experimental periods. The SID values were determined by difference to SID values in casein. The results of the present studies show large variations in SID values among differently processed soybean products (p0.05). The SID values in extruded soybeans (SBe) were lower compared to high-protein soybean meal (SBMhp), soy protein concentrate (SPC) or partially hydrolysed soy protein isolate (SPIh) (p0.05). Aqueous alcohol or water extraction in SPC or SPIh improved SID values compared to SBMhp (p0.05). The SID values among the 4 batches of SPC were similar (p0.05) and consistently high. Moreover, the SID values were similar in SPC and SPIh (p0.05). The SID values of in alternatively processed soy proteins including enzymatically fermented SPA and microbially fermented SPB, were inconsistent. For the 3 SPA products, SID increased numerically from values similar to SBMhp to values similar to SPC with increasing duration of enzymatic treatment. For SPB SID values were low and similar to SBe, which may be attributed to protein denaturation during microbial fermentation. The results of present study show for most AA similar SID values for all by-products of starch processing including pea protein (PeaP), wheat gluten (WG), corn gluten (CG), and potato protein (PotP) compared to SPC (p0.05) except for SID of Lys in WG and CG, and SID of Trp in CG were considerably lower compared to other products (p0.05). The particular low SID of Lys and Trp in WG and CG might be simply a reflection of an experimental error, because SID values by means of the difference method rely on sufficient high AA contribution levels to the assay diets, which was not achieved for the aforementioned AA. The results of the present study show that SID values in fish meal (FM1) and extracted fish meal (FM2e) vary considerably (p
Author: Pedro E. Urriola
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
ABSTRACT: In vivo digestibility and in vitro digestibility experiments were used to study effects of feeding distillers dried grains with solubles (DDGS) and other high fiber ingredients to growing pigs. The objective of Exp. 1 was to measure the effects on digestibility of AA, energy, and fiber of adding 30% DDGS to a corn soybean meal diet and to measure intestinal transit time. Growing pigs were cannulated at the terminal ileum and in the cecum. Results showed that apparent ileal digestibility (AID) of Lys (74.1%) was reduced (P
