In-situ Bioremediation of Methyl Tertiary Butyl Ether (MTBE) PDF Download
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Author: Naval Facilities Engineering Service Center
Publisher:
ISBN:
Category : Bioremediation
Languages : en
Pages : 2
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Book Description
Author: Naval Facilities Engineering Service Center
Publisher:
ISBN:
Category : Bioremediation
Languages : en
Pages : 2
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Equilon's Westhollow Technology Center teamed with Arizona State University to evaluate an in-situ process for bioremediation of methyl tertiary butyl ether (MTBE). MTBE is a gasoline additive that was introduced in fuels to reduce emission of a number of air pollutants including carbon monoxide (CO). Navy sites where MTBE contamination is present. Since MTBE is required in regions of the country that exceed ambient standards for CO, it often is found in urban areas. The U.S. EPA issued a health advisory for MTBE in 1997 indicating that the drinking water level should be less than 40 micro g/L based in odor and taste thresholds. Recent laboratory experiments and limited field studies have demonstrated that MTBE can be aerobically degraded by a bacterial culture, BC-4, developed by the Shell Development Company. BC-4 grows naturally in activated sludge at industrial wastewater treatment plants. The BC-4 culture is a mixture of ordinary soil bacteria such as coryneforms, pseudomonads, and achromobacter species that have been acclimated to MTBE for more than a year. Bioreactor studies with BC-4 in the presence of oxygen have shown 99% removal of MTBE from groundwater, based on a 25-hour retention time. The end products of the degradation are carbon dioxide and water. The field trial, which is the first of its kind for treatment of MTBE, is focusing on the use of BC-4 as an in-situ bio-barrier to downgradient MTBE migration.
Author: Kristin Adair Hicks
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
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Author: Laura K. G. Youngster
Publisher:
ISBN:
Category : Butyl methyl ether
Languages : en
Pages : 132
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Book Description
Groundwater contamination with the synthetic fuel oxygenate, methyl tert-butyl ether (MTBE), is an extensive problem. Microbial mediated biodegradation holds promise as a tool for remediation of contaminated water supplies. However, MTBE biotransformation processes are slow and MTBE degrading organisms are difficult to isolate, creating challenges relating to site assessment, enhancement of natural attenuation and monitoring bioremediation in situ. In this study we analyzed MTBE degrading cultures using a variety of isolation independent techniques. A majority of the experiments used previously established anaerobic enrichment cultures that had been maintained on MTBE for several years. We demonstrated that low concentrations of some aryl O-methyl ether compounds enhanced the rate of MTBE degradation. Propyl iodide caused a light-reversible inhibition of MTBE depletion, suggesting that the anaerobic MTBE O-demethylation reaction was corrinoid dependent. Terminal-restriction fragment length polymorphism (T-RFLP) and sequence analysis of 16S rRNA genes from one anaerobic MTBE degrading enrichment culture showed a phylogenetically diverse population with no exact matches to previously isolated or described species. Stable isotope probing experiments verified that microorganisms from anaerobic MTBE degrading enrichment culture used 13C from 13C-MTBE for growth and cell division and that a particular subpopulation assimilated this carbon prior to the rest of the population. We also analyzed carbon and hydrogen stable isotope fractionation occurring during MTBE degradation. In anaerobic cultures, substantial fractionation of hydrogen was found only in cultures supplied with syringic acid during MTBE degradation, providing the first experimental suggestion of multiple anaerobic MTBE O-demethylation mechanisms. During aerobic MTBE degradation by the psychrophilic bacterium, Variovorax paradoxus, carbon and hydrogen fractionation were not influenced by incubation temperature during degradation. This work represents a significant contribution to the current body of knowledge about MTBE degradation and the data presented will be useful in many aspects of studying, enhancing and monitoring MTBE degradation under a variety of conditions.
Author: Julian Scott Kayne
Publisher:
ISBN:
Category :
Languages : en
Pages : 166
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Author: Victor Magar
Publisher:
ISBN:
Category : Technology & Engineering
Languages : en
Pages : 272
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- Biobarriers and Characterization of MTBE-Degrading Cultures- Natural Attenuation of MTBE- In Situ MTBE Biodegradation- MTBE Cometabolism
Author: Tong Liu
Publisher:
ISBN:
Category : Butyl methyl ether
Languages : en
Pages : 109
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Book Description
The wide use of methyl tert-butyl ether (MTBE), a synthetic fuel oxygenate, have caused extensive contamination in groundwater in past two decades and resulted in taste, odor, and potential toxicity problems in drinking water. MTBE contaminated groundwater aquifer is often depleted of oxygen and microorganisms can anaerobically degraded MTBE under a verity of redox conditions. It has been demonstrated that MTBE can be degraded anaerobically which makes in situ bioremediation of contaminated aquifers a potential solution to address this problem. Assessment and enhancement of MTBE bioremediation requires knowledge of the microorganisms that responsible for biodegradation process. However, it is challenge to isolate anaerobic MTBE-degrading microorganisms or to characterize the microbial communities. The aim of this study was to identify the organisms that mediate anaerobic biodegradation of MTBE in methanogenic or sulfidogenic cultures enriched from estuarine sediments. Stable isotope probing (SIP) combined with terminal restriction fragment length polymorphism (T-RFLP) analysis showed that Ruminococcaceae species were active in methanogenic MTBE-degrading community. T-RFLP coupled with clone library analysis of bacterial 16S rRNA genes from sulfidogenic enrichment cultures showed Deltaproterobacteria were highly enriched than the other phylogenetically diverse populations. We also investigated MTBE-degrading communities by single cell using Raman Spectroscopy and SIP. The results confirmed the MTBE degraders are not abundant in the communities. This study provides crucial information for understanding the mechanisms of anaerobic degradation of MTBE as well as for assessment of the in situ bioremediation at contaminated field sites as the microbial/molecular tools.
Author:
Publisher:
ISBN:
Category : Butyl methyl ether
Languages : en
Pages : 52
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Author: Stephanie M. Smith
Publisher:
ISBN:
Category :
Languages : en
Pages : 258
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Author: Ellen Moyer
Publisher: Springer Science & Business Media
ISBN: 9781884940293
Category : Science
Languages : en
Pages : 720
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Book Description
The MTBE Remediation Handbook is a comprehensive and up-to date compendium of knowledge of the technology and risk management of MTBE contamination. This handbook examines the remediation of MTBE in existing spills: exploring the myths which act as impediments to successful clean-up techniques, and offering effective solutions. Experience in the last decade has shown that prompt source control is key to minimizing impacts and remediation costs. Successful treatment of contamination depends on the selection of the appropriate technology, well done site characterization, sound engineering design and implementation. The focus of this volume is the remediation of MTBE in existing spills. Section I of the MTBE Remediation Handbook features an in-depth look at the history, properties, occurrence and assessment of MTBE. Section II discusses applicable remediation technologies. Section III offers remediation case studies. The MTBE Remediation Handbook presents environmental scientists and cleanup professionals an indispensable resource on the handling of MTBE contamination worldwide.
