Identification of Genes Required for Growth of Saccharomyces Cerevisiae Under the Ethanol Stress PDF Download
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Author: Renko Goyashiki
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
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Book Description
Author: Renko Goyashiki
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
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Book Description
Author: Sylvie Callegari
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 152
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Book Description
Saccharomyces cerevisiae express a high degree of ethanol tolerance compared to other microorganisms. The complete mechanism involved in this tolerance has not yet been fully identified, but is likely to have evolved during naturally occurring fermentations in the wild. Microarray data has revealed a total of 24 genes up-related greater than three-fold in cells that have adapted to 5-6% ethanol. These genes may potentially have a role in the ethanol stress mechanisms of S. cerevisiae; however, previous studies examining genome/phenome relationship under stress conditions have failed to establish a correlation between gene up-regulation and function. To analyse the genome/phenome relationship for ethanol stress, deletion mutants were obtained and confirmed by PCR for several of the genes identified in the microarray data. They were then assayed using a series of ethanol assays differing in range and sensitivity. Nine of the ten deletion mutants assayed revealed an ethanol susceptible phenotype and encode proteins with a range of functions including wall synthesis, protein stabilisation, maintenance of ionic homeostasis and response to nutrient starvation. These are believed to have a role in combating ethanol stress. Results have also given an insight into the complexity of the S. cerevisiae mechanisms of ethanol tolerance, revealing that despite being commonly up-regulated, the genes have very diverse roles in which phenotypes are condition dependant. Contrary to previous studies a genome/phenome relationship has been detected for a great majority of the ethanol induced genes assayed, signifying the value of a comprehensive and sensitive system for the functional analysis of genes.
Author: Sophia Seh-Yi Su
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Book Description
Author: Fred W. Allendorf
Publisher: John Wiley & Sons
ISBN: 1444309056
Category : Science
Languages : en
Pages : 664
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Book Description
Conservation and the Genetics of Populations gives acomprehensive overview of the essential background, concepts, andtools needed to understand how genetic information can be used todevelop conservation plans for species threatened withextinction. Provides a thorough understanding of the genetic basis ofbiological problems in conservation. Uses a balance of data and theory, and basic and appliedresearch, with examples taken from both the animal and plantkingdoms. An associated website contains example data sets and softwareprograms to illustrate population genetic processes and methods ofdata analysis. Discussion questions and problems are included at the end ofeach chapter to aid understanding. Features Guest Boxes written by leading people in the fieldincluding James F. Crow, Nancy FitzSimmons, Robert C. Lacy, MichaelW. Nachman, Michael E. Soule, Andrea Taylor, Loren H. Rieseberg,R.C. Vrijenhoek, Lisette Waits, Robin S. Waples and AndrewYoung. Supplementary information designed to support Conservationand the Genetics of Populations including: Downloadable sample chapter Answers to questions and problems Data sets illustrating problems from the book Data analysis software programs Website links An Instructor manual CD-ROM for this title is available. Pleasecontact our Higher Education team at ahref="mailto:
[email protected]"
[email protected]/afor more information.
Author: Lisa P. Elia
Publisher:
ISBN:
Category :
Languages : en
Pages : 442
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Book Description
Author: Isidora Sophia Ishtar Snoek
Publisher:
ISBN: 9789074384063
Category :
Languages : en
Pages : 149
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Book Description
Author: Stefan Hohmann
Publisher: Springer Science & Business Media
ISBN: 3540456112
Category : Science
Languages : en
Pages : 398
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Book Description
Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.
Author: Tina Thi My Tien Tran
Publisher:
ISBN:
Category : Ethanol
Languages : en
Pages :
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Book Description
S. cerevisiae has evolved the ability to tolerate high concentrations of ethanol, a trait that has contributed to this yeast being a cornerstone of beverage and biofuel industries. The key genes involved in conferring ethanol tolerance in S. cerevisiae are unknown. One strategy used to identify genes conferring ethanol tolerance, was to create ethanol-tolerant (ET) mutants from a laboratory yeast strain and use these to identify ethanol-tolerance conferring loci. Dr. Dragana Stanley (Victoria University) created two ET mutants, a spontaneous (SM) and a chemical (CM) mutant from W303, via adaptive evolution (Stanley 2008). Transcriptome analysis of the resultant ET mutants found that expression levels of hundreds of genes were altered, relative to the parent under ethanol-stress conditions. The primary objective of this thesis was to identify genes that confer ethanol tolerance in SM and CM.
Author: Antonio Morata
Publisher: BoD – Books on Demand
ISBN: 9535135996
Category : Technology & Engineering
Languages : en
Pages : 302
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Book Description
Yeast - Industrial Applications is a book that covers applications and utilities of yeasts in food, chemical, energy, and environmental industries collected in 12 chapters. The use of yeasts in the production of metabolites, enzymatic applications, fermented foods, microorganism controls, bioethanol production, and bioremediation of contaminated environments is covered showing results, methodologies, and processes and describing the specific role of yeasts in them. The traditional yeast Saccharomyces cerevisiae is complemented in many applications with the use of less known non-Saccharomyces yeasts that now are being used extensively in industry. This book compiles the experience and know-how of researchers and professors from international universities and research centers.
Author: Graeme M. Walker
Publisher: John Wiley & Sons
ISBN: 0471964468
Category : Science
Languages : en
Pages : 375
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Book Description
Yeasts are the world's premier industrial micro-organisms. In addition to their wide exploitation in the production of foods, beverages and pharmaceuticals, yeasts also play significant roles as model eukaryotic cells in furthering our knowledge in the biological and biomedical sciences. In order for modern biotechnology to fully exploit the activities of yeasts, it is essential to appreciate aspects of yeast cell physiology. In recent years, however, our knowledge of yeast physiological phenomena has lagged behind that of yeast genetics and molecular biology. Yeast Physiology and Biotechnology redresses the balance by linking key aspects of yeast physiology with yeast biotechnology. Individual chapters provide broad and timely coverage of yeast cytology, nutrition, growth and metabolism - important aspects of yeast cell physiology which are pertinent to the practical uses of yeasts in industry. The final chapter reviews traditional, modern and emerging biotechnologies in which roles of yeasts in the production of industrial commodities and their value in biomedical research are fully discussed. Relevant aspects of classical and modern yeast genetics and molecular biology are fully integrated into the appropriate chapters. This up-to-date and fully referenced book is aimed at advanced undergraduate and postgraduate bioscience students,but will also prove to be a valuable source of information for yeast researchers and technologists.
