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Author: Diana Carolina Mazo Molina
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
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Book Description
Pseudomonas syringae pv. tomato (Pst) is a persistent pathogen of tomato that causes bacterial speck disease. On tomato, resistance conferred by the gene Pto is effective against race 0 Pst strains which express the effector proteins AvrPto and/or AvrPtoB; however, race 1 strains of Pst, which do not express AvrPto/AvrPtoB but rather a different repertoire of effectors, evade Pto-mediated resistance. Race 1 strains of Pst are becoming increasingly common, and no simply-inherited genetic resistance to such strains is known. It was discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 Pst strains by recognizing the type III effector AvrRpt2. In Arabidopsis and apple, strains of Pst and Erwinia amylovora expressing AvrRpt2 degrade the RIN4 protein, thereby activating RPS2 or Mr5-mediated immunity, respectively. Ptr1 also recognized homologs of AvrRpt2 from diverse bacteria including one in Ralstonia pseudosolanacearum and this correlated with the ability of AvrRpt2 to degrade RIN4. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 detection of AvrRpt2 activity suggests it likely encodes an NLR protein or possibly a guardee such as RIN4. Ptr1 was identified by cloning of candidate NLR-encoding genes located in the Ptr1 region and testing using Agrobacterium-mediated transient expression in Nicotiana glutinosa identified one gene for the ability to activate the plant immune system in response to AvrRpt2 in the presence of tomato Rin4. Interestingly, while overexpression of Ptr1 in N. glutinosa leaves caused localized cell death, co-expression of Ptr1 with tomato Rin4 prevented this cell death. The protein encoded by Ptr1 has little similarity to RPS2 or Mr5, which suggests that Ptr1 is a third example of convergent evolution in different plant species for recognition of AvrRpt2. In summary, the Ptr1 gene has the potential to become an important component (along with Pto) in controlling bacterial speck disease. Further research focused on studying the mechanism of action between Ptr1 and Rin4 may contribute to a better understanding of the recognition of the type III effector AvrRpt2 in tomato.
Author: Diana Carolina Mazo Molina
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
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Book Description
Pseudomonas syringae pv. tomato (Pst) is a persistent pathogen of tomato that causes bacterial speck disease. On tomato, resistance conferred by the gene Pto is effective against race 0 Pst strains which express the effector proteins AvrPto and/or AvrPtoB; however, race 1 strains of Pst, which do not express AvrPto/AvrPtoB but rather a different repertoire of effectors, evade Pto-mediated resistance. Race 1 strains of Pst are becoming increasingly common, and no simply-inherited genetic resistance to such strains is known. It was discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 Pst strains by recognizing the type III effector AvrRpt2. In Arabidopsis and apple, strains of Pst and Erwinia amylovora expressing AvrRpt2 degrade the RIN4 protein, thereby activating RPS2 or Mr5-mediated immunity, respectively. Ptr1 also recognized homologs of AvrRpt2 from diverse bacteria including one in Ralstonia pseudosolanacearum and this correlated with the ability of AvrRpt2 to degrade RIN4. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 detection of AvrRpt2 activity suggests it likely encodes an NLR protein or possibly a guardee such as RIN4. Ptr1 was identified by cloning of candidate NLR-encoding genes located in the Ptr1 region and testing using Agrobacterium-mediated transient expression in Nicotiana glutinosa identified one gene for the ability to activate the plant immune system in response to AvrRpt2 in the presence of tomato Rin4. Interestingly, while overexpression of Ptr1 in N. glutinosa leaves caused localized cell death, co-expression of Ptr1 with tomato Rin4 prevented this cell death. The protein encoded by Ptr1 has little similarity to RPS2 or Mr5, which suggests that Ptr1 is a third example of convergent evolution in different plant species for recognition of AvrRpt2. In summary, the Ptr1 gene has the potential to become an important component (along with Pto) in controlling bacterial speck disease. Further research focused on studying the mechanism of action between Ptr1 and Rin4 may contribute to a better understanding of the recognition of the type III effector AvrRpt2 in tomato.
Author: Francine Mary Garland
Publisher:
ISBN:
Category :
Languages : en
Pages : 284
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Book Description
Author: Christine Maria Kraus
Publisher:
ISBN:
Category :
Languages : en
Pages : 234
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Book Description
The use of natural variation is a powerful tool to study the evolution of plant resistance genes and pathogen effectors. In tomato, the Pto protein kinase interacts directly with two Pseudomonas syringae pv. tomato (Pst) effectors, AvrPto and AvrPtoB. This direct interaction is mediated by the binding of each effector through both a shared and a unique interface with Pto. The presence of two unique interfaces suggested that the recognition of these two effectors by Pto might have evolved independently. We conducted a screen of wild tomato accessions for their ability to mount effector-triggered immunity upon recognition of AvrPto or AvrPtoB to seek evidence of natural variation that would shed further light on how Pto-like kinases recognize and respond to two structurally different effectors. Our screen of wild relatives of tomatoes uncovered 22 accessions of Solanum chmielewskii (Schm) that recognize only AvrPtoB. Through further molecular characterization we found that a single histidine-to-aspartate substitution at position 193 in the activation domain of Schm Pto-2677 was sufficient to confer recognition of AvrPto in plant cells. The reciprocal substitution of aspartate-to-histidine-193 in Pto abolished AvrPto recognition, confirming the importance of this residue for signaling in response to AvrPto. Our results reveal that there are not only distinct binding interfaces involved in the Pto response to these effectors, but that there is also a difference in downstream signaling. Based on recent worldwide collections of Pst isolates it is known that race 1 strains have displaced race 0 strains and are now the most common strains found in the field. Race 0 strains express AvrPto or AvrPtoB and elicit resistance in Pto-expressing tomato lines, whereas race 1 strains lack these effectors and do not elicit resistance. We screened Pst isolates from infected field tomato plants across New York (NY) in 2015 and characterized them for their virulence and for the presence of specific effectors. We found that all isolates encode a functional AvrPto, which can be recognized by Pto. However, this recognition is 'masked' during later stages of infection, allowing development of mild bacterial speck symptoms in Pto-expressing tomatoes when vacuum infiltrated with high bacterial populations and under laboratory conditions. Our study demonstrates that introgression of Pto is still a viable strategy to manage bacterial speck of tomatoes in NY. ...
Author: Aurelio Ciancio
Publisher: Springer Science & Business Media
ISBN: 1402085710
Category : Technology & Engineering
Languages : en
Pages : 436
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Book Description
This volume focuses on integrated pest and disease management (IPM/IDM) and biocontrol of some key diseases of perennial and annual crops. It continues a series originated during a visit of prof. K. G. Mukerji to the CNR Plant Protection Institute in Bari (Italy), in November 2005. Both editors aim at a series of five volumes embracing, in a multi-disciplinary approach, advances and achievements in the practice of crop protection, for a wide range of plant parasites and pathogens. Two volumes of the series were already produced, dedicated to general concepts in IPM and to management and biocontrol of nematodes of grain crops and vegetables. This Volume deals, in particular, with diseases due to bacteria, phytoplasma and fungi. Every day, in any agroecosystem, farmers face problems related to plant diseases. Since the beginning of agriculture, indeed, and probably for a long time in the future, farmers will continue to do so. Every year, plant diseases cause severe losses in the global production of food and other agricultural commodities, worldwide. Plant diseases are not limited to episodic events occurring in single farms or crops, and should not be regarded as single independent cases, affecting only farms on a local scale. The impact of plant disease epidemics on food shortage ignited, in the last two centuries, deep cultural, social and demographic changes, affecting million human beings, through i. e. migration, death and hunger.
Author: Satbir Singh Gosal
Publisher: Springer Nature
ISBN: 3030472981
Category : Science
Languages : en
Pages : 457
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Book Description
Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This edited volume summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.
Author: M K Razdan
Publisher: CRC Press
ISBN: 1578081793
Category : Technology & Engineering
Languages : en
Pages : 666
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Book Description
Potato is the most significant non-cereal crop. Much attention has been paid to this commercially important crop. The aim of this volume is to capture the recent advances made in improving potatoes using traditional breeding methods as well as genetic engineering technology. The book provides a critical appraisal of the state-of-the-art finding on this crop.
Author: Mathilde Causse
Publisher: Springer
ISBN: 3662533898
Category : Science
Languages : en
Pages : 260
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Book Description
This book describes the strategy used for sequencing, assembling and annotating the tomato genome and presents the main characteristics of this sequence with a special focus on repeated sequences and the ancestral polyploidy events. It also includes the chloroplast and mitochondrial genomes. Tomato (Solanum lycopersicum) is a major crop plant as well as a model for fruit development, and the availability of the genome sequence has completely changed the paradigm of the species’ genetics and genomics. The book describes the numerous genetic and genomic resources available, the identified genes and quantitative trait locus (QTL) identified, as well as the strong synteny across Solanaceae species. Lastly, it discusses the consequences of the availability of a high-quality genome sequence of the cultivated species for the research community. It is a valuable resource for students and researchers interested in the genetics and genomics of tomato and Solanaceae.
Author: M K Razdan
Publisher: CRC Press
ISBN: 1439844062
Category : Science
Languages : en
Pages : 621
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Book Description
Potato is the most significant non-cereal crop. Much attention has been paid to this commercially important crop. The aim of this volume is to capture the recent advances made in improving potatoes using traditional breeding methods as well as genetic engineering technology. The book provides a critical appraisal of the state-of-the-art finding on
Author: I. R. Crute
Publisher: Cabi
ISBN:
Category : Medical
Languages : en
Pages : 456
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Book Description
Genetics has transformed plant pathology on two occasions: first when Mendelian genetics enabled the discovery that disease resistance was a heritable trait in plants, and secondly when Flor proposed the "gene-for-gene" hypothesis to explain his observations of plant-parasite interactions, based on his work on flax rust in North Dakota starting in the 1930s. Our knowledge of the genetics of disease resistance and host-pathogen coevolution is now entering a new phase as a result of the cloning of the first resistance genes. This book provides a broad review of recent developments in this important and expanding subject. Both agricultural and natural host-pathogen situations are addressed. While most of the book focuses on plant pathology, in the usual sense of the term embracing fungal, bacterial and viral pathogens, there is also consideration of parasitic plants and a chapter demonstrating lessons to be learnt from the mammalian immune system. Three overall themes are addressed: genetic analyses and utilization of resistance; population genetics; and cell biology and molecular genetics. Chapters are based on papers presented at the British Society for Plant Pathology Presidential meeting held in December 1995, but all have been revised and updated to mid-1996. Written by leading authorities from North America, Europe and Australia, the book represents an essential update for workers in plant genetics, breeding, biotechnology and pathology.
Author: Sangita Sahni
Publisher: CRC Press
ISBN: 1003828604
Category : Technology & Engineering
Languages : en
Pages : 492
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Book Description
Crop protection is becoming increasingly important due to the need for food security and to meet growing environmental challenges. This new book, New Advances in Crop Disease Management, provides readers with an updated and comprehensive overview of diverse crop protection strategies with special emphasis on the recent advances in crop protection approaches, fundamental concepts, principles, methodology, relationships, and benefits for crop protection and human health. It covers a variety of disciplines such as plant pathology, biochemistry, cell, and molecular biology, plant physiology, host-pathogen interactions, precision agriculture, decision analysis, disease risk, new ways of exploiting host genetic diversity including host resistance deployment, biological control and microbial interactions, surveillance and detection technologies, and the consequences of climate change and signaling. The book contains twenty chapters and begins with the role of biotechnology and its implications in crop disease detection and protection. It then discusses the plant response to pathogen attack, molecular basis of host-pathogen interaction and induction of defense response, viz., R gene, phytoalexins, etc., and advancement in early detection methodologies. It also delves into induced mutation as new technology for resistant crop development. The last part details the recent approaches and applications of resistant crop lines development, including resistant pulses development approaches, mining and mapping of R genes, gene pyramiding, lactic acid bacteria as biocontrol agents, effecteromics and nanotechnology in crop disease management, and the applications of phytochemicals and CRISPR-Cas in the development of disease resistant crops for future. New Advances in Crop Disease Management will be a key reference in the field of crop science and plant pathology, agriculture for faculty and industry professionals as well as a valuable textbook/reference for undergraduate and graduate students.
