Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 0

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Author: Hans Ramløv
Publisher: Springer Nature
ISBN: 3030419290
Category : Science
Languages : en
Pages : 318

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Book Description
This first volume provides a comprehensive overview on evolutionary, environmental and systematic aspects of antifreeze proteins. It shortly explains the physical properties of ice and further intelligibly describes the biology of the antifreeze proteins in different organisms, and offers a detailed insight into their history of evolution. In addition the book discusses the status of the current knowledge and ongoing research and highlights also those parts, where further investigation needs to be done. Together with the second volume on the biochemistry and molecular biology of antifreeze proteins, this book represents a unique, comprehensive work and a must-have for students and scientists in biochemistry, evolution, physiology and physical chemistry.

Author: Ran Drori
Publisher: Springer Nature
ISBN: 1071635034
Category : Science
Languages : en
Pages : 233

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Book Description
This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins. Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and synthesize mimics of IBPs, as well as applications involving these proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Ice Binding Proteins: Methods and Protocols aims to provide both experimental and simulations protocols to help new researchers, break-the-ice, and enter this exciting field, while also supporting established researchers broaden the scope of their investigations.

Author: Sally Oi Wah Yu
Publisher:
ISBN:
Category :
Languages : en
Pages : 202

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Book Description
Antifreeze proteins (AFPs) help cold-adapted organisms survive below 0°C by binding to and inhibiting the growth of ice crystals. In this way, AFPs depress the freezing point of aqueous fluids below the melting point of ice (thermal hysteresis; TH). They also have the ability to inhibit ice recrystallization in the frozen state (ice recrystallization inhibition; IRI). Some AFPs show an order of magnitude higher TH activity than others, and are termed 'hyperactive'. One of the objectives of this thesis was to see if IRI activities of the hyperactive AFPs are also an order of magnitude higher than the moderately active AFPs. Using a capillary-based assay for IRI, the activities of three hyperactive and three moderately active AFPs were determined. There was no apparent correlation between hyperactivity in TH and high IRI activity. However, mutations of residues on the ice-binding face (IBF) of both types of AFP reduced IRI and TH activities to a similar extent. In this way, the use of IBF mutant AFPs showed that the IBF responsible for an AFP's TH activity is also responsible for its IRI activity. Analysis of the diverse AFP structures solved to date indicate that their IBFs are relatively flat, occupy a significant proportion of the protein's surface area and are more hydrophobic than other surfaces of the protein. The IBFs also often have repeating sequence motifs and tend to be rich in alanine and/or, threonine. The de novo design of an ice-binding protein was undertaken using these features to verify the underlying physicochemical requirements necessary for a protein's interaction with ice. Using site-directed mutagenesis, a total of sixteen threonine substitutions were made on one of the four faces of a cyanobacterial protein with no endogenous TH activity. The inclusion of eight paired threonines on one face of this quadrilateral helix gave the engineered protein low levels of TH activity, but at the cost of destabilizing the structure to some extent. The results of this study have validated some of the properties needed for the ice-binding activity of AFPs.

Author: Steffen P. Graether
Publisher: Nova Science Publishers
ISBN: 9781621004356
Category : Antifreeze proteins
Languages : en
Pages : 0

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Book Description
Antifreeze proteins, also known as thermal hysteresis proteins, ice binding proteins and ice structuring proteins, prevent the growth of ice crystals in several cold blooded organisms. First discovered in fish, they have also been found in insects, plants, fungi and bacteria. Antifreeze proteins cause the non-colligative depression of the freezing point of water, a property which has been exploited in the practical applications of antifreeze proteins such as improving the texture of ice cream, and could be used to extend the crop growing season or allow fish to thrive in cold waters. This book provides clear information on what is known about antifreeze proteins today and how to study them.

Author: Kathryn Vanya Ewart
Publisher: World Scientific
ISBN: 9810248997
Category : Nature
Languages : en
Pages : 249

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Book Description
A collection of works by researchers who have studied the antifreeze proteins which enable organisms to avoid freezing under extreme conditions.

Author: Rosa Margesin
Publisher: Springer Science & Business Media
ISBN: 3662062852
Category : Science
Languages : en
Pages : 408

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Book Description
Representing the latest knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, this book explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.

Author: Reeta Goel
Publisher: Springer Nature
ISBN: 9811626251
Category : Science
Languages : en
Pages : 435

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Book Description
Extremophiles have unique physiological properties, thus considered to be ideal candidates for industrial development. This book present concepts on cold-adapted microorganisms, centered on four different aspects - (i) diversity of cold adapted microbes (ii) their ecology, physiology and metabolism (iii) omics research in the field and (iv) their potential applications. This volume collates the recent developments and innovations with respect to these microorganisms. This book is meant for researchers, biochemists, industries, and government agencies interested in cold active microbes and their products. Also, would be of interest to NGOs and progressive farmers which are working for higher altitude ecosystems throughout the globe.

Author: Rosa Margesin
Publisher: Springer Science & Business Media
ISBN: 9783540649731
Category : Medical
Languages : en
Pages : 432

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Book Description
Major parts of the oceans and lands of our planet are permanently, or temporarily, exposed to temperatures below 10 C. Microorganisms, plants and animals living under these conditions have adapted to their environments in such a way that metabolic processes, reproduction and survival strategies are optimal for their natural biotopes. This book presents the most recent knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, and explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.

Author: Dirk K. Hincha
Publisher: Humana
ISBN: 9781493908431
Category : Science
Languages : en
Pages : 0

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Book Description
Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.