Author: Isadore B. Berlman
Publisher:
ISBN:
Category : Aromatic compounds
Languages : en
Pages : 280

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Author: Isadore Berlman
Publisher: Elsevier
ISBN: 0323161677
Category : Science
Languages : en
Pages : 488

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Book Description
Handbook of Fluorescence Spectra of Aromatic Molecules, Second Edition describes the fluorescence and absorption spectra of about 200 aromatic compounds, most of which fall into the following classes: p-oligophenylenes, indole derivatives, fluoranthene derivatives, napthalene derivatives, biphenyl derivatives, and biological stains. Experiments with lasers and their relevance to fluorescence studies are included. This handbook is comprised of seven chapters and begins with a historical overview of research into the fluorescence characteristics of compounds, the methods and equipment used to measure fluorescence, and elementary considerations concerning luminescence. The format for the presentation of data pertaining to each compound covered in this text is described, together with the equipment for exciting, detecting, and recording the spectrum of the emitted radiation. The discussion then turns to the free electron model and presents general information on concepts such as chromophores, planar and nonplanar molecules, effects of planarity on fluorescence, solvent and concentration effects, and polarization. The following chapters focus on compounds such as benzene and polycyclic hydrocarbons as well as some uses of fluorescent compounds. This monograph will be of interest to organic chemists and physicists.

Author: Jacob Malkin
Publisher: CRC Press
ISBN: 9780849368028
Category : Science
Languages : en
Pages : 446

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Book Description
Photophysical and Photochemical Properties of Aromatic Compounds is the first book to collect and classify all available quantitative data on the photochemistry and luminescence of aromatic compounds. Compounds are classified by both spectral-luminescent (e.g., extinction coefficients, energies and lifetimes of lower excited states) and photochemical properties. In addition, all of the quantum yields available have been collected. The variety of photochemical reactions of aromatics is examined based on eight types of elementary monomolecular and bimolecular photochemical processes. Aromatic compounds are grouped into eight categories, and the book analyzes the possibilities of occurrence of all types of elementary photoprocesses.

Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 146157658X
Category : Science
Languages : en
Pages : 487

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Book Description
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Author:
Publisher:
ISBN:
Category : Nuclear energy
Languages : en
Pages : 1558

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Author: Ammasi Periasamy
Publisher: CRC Press
ISBN: 1420078917
Category : Science
Languages : en
Pages : 474

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Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be

Author: K. D. Mielenz
Publisher:
ISBN:
Category : Fluorimetry
Languages : en
Pages : 172

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Author: Stephen G Schulman
Publisher: Elsevier
ISBN: 1483160998
Category : Science
Languages : en
Pages : 299

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Book Description
Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.

Author: Partha Pratim Mondal
Publisher: Springer Science & Business Media
ISBN: 9400775458
Category : Science
Languages : en
Pages : 219

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Book Description
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Author:
Publisher:
ISBN:
Category : Commerce
Languages : en
Pages : 176

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