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Author: Chiyedza Small
Publisher:
ISBN: 9781524988159
Category :
Languages : en
Pages :
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Book Description
Author: Chiyedza Small
Publisher:
ISBN: 9781524988159
Category :
Languages : en
Pages :
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Book Description
Author: Gregore Koliantz
Publisher:
ISBN: 9780891185611
Category : Electronic books
Languages : en
Pages : 0
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Book Description
Cell Division(Mitosis): Looking at Chromosomes -- Restriction Testing -- Extraction of Genomic DNA -- Polymerase Chain Reaction -- Cloning 1 Generating Recombinant Plasmids -- Cloning 2 Minipreping -- Southern Blotting 1 DNA Transfer -- Southern Blotting 2 DNA-DNA Hybridization and Sequence Detection -- Regulation of Gene Expression -- Physical Properties of DNA and DNA Assay -- Transmission Genetics (Heredity) -- Meiosis and Analysis of Crossing Over -- Complementation Test -- Molecular Markers: Mapping the Genome of Arabidopsis -- Population Genetics How Changes Occur within a Population -- Front Matter.
Author: Ernest Brown Babcock
Publisher:
ISBN:
Category : Breeding
Languages : en
Pages : 76
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Book Description
Author: Dennis Ray
Publisher:
ISBN: 9780757597695
Category : Science
Languages : en
Pages : 30
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Book Description
Author: Dennis Ray
Publisher: Kendall/Hunt Publishing Company
ISBN: 9780757564826
Category : Science
Languages : en
Pages : 218
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Book Description
Author: Melody S. Clark
Publisher: Springer Science & Business Media
ISBN: 3642878733
Category : Science
Languages : en
Pages : 551
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Book Description
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author: Ammar Al-Chalabi
Publisher:
ISBN: 9780879698829
Category : Medical genetics
Languages : en
Pages : 0
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Book Description
Many human diseases – including Alzheimer's disease, schizophrenia, cancer, and cardiovascular disease – have complex genetics that require sophisticated analysis. Genetics of Complex Human Diseases: A Laboratory Manual& brings together the tools that geneticists use to find disease genes with the genetic concepts and statistical theories that underpin these research approaches. Topics covered include basic genetics and Mendelian inheritance, statistical methods, genetic epidemiology, linkage studies, transmission disequilibrium test analysis, variance components analysis, genome-wide association studies, copy-number variation, methods for high-throughput genotyping, the complexity of RNA editing, and genetic computer programs. The book's chapters, written by leading investigators in the field, blend practical information and reviews of each topic, providing both the how and the why of complex disease analysis. Genetics of Complex Human Diseasesis an important guide for anyone with an interest in human genetics or who uses genetic techniques in the study of diseases with complex inheritance.
Author: Marilyn S. Arsham
Publisher: John Wiley & Sons
ISBN: 1119061229
Category : Science
Languages : en
Pages : 1216
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Book Description
Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Author: Rodney J. Scott
Publisher:
ISBN: 9780895825711
Category : Science
Languages : en
Pages : 116
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Book Description
Author: Heather B. Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 227
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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
