Functional Analysis of Codon Bias in the Alcohol Dehydrogenase Gene of Drosophila Melanogaster PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Functional Analysis of Codon Bias in the Alcohol Dehydrogenase Gene of Drosophila Melanogaster PDF full book. Access full book title Functional Analysis of Codon Bias in the Alcohol Dehydrogenase Gene of Drosophila Melanogaster by Nathan Ray Anderson. Download full books in PDF and EPUB format.
Author: Nathan Ray Anderson
Publisher:
ISBN: 9781109795929
Category : Drosophila melanogaster
Languages : en
Pages : 44
Get Book Here
Book Description
Codon bias and its effect on protein production was studied using the model organism Drosophila melanogaster. Seven preferred leucine codons in the alcohol dehydrogenase (Adh) gene were changed to unpreferred codons. This gene (7-Up) was microinjected into a line of flies to create mutants carrying the mutant gene. Total soluble protein was isolated and assayed for total protein present and activity level of the enzyme. Ten control lines with the wildtype Adh gene and twelve mutant lines with the 7-Up gene showed average activity levels of 112.8 +/- 28.8 and 124.1 +/- 57.3 mumol NAD+ reduced per minute per mug of protein x 100, respectively. These results indicate that increasing codon bias artificially has no effect on protein production, possibly due to saturated tRNA pools in the cell.
Author: Nathan Ray Anderson
Publisher:
ISBN: 9781109795929
Category : Drosophila melanogaster
Languages : en
Pages : 44
Get Book Here
Book Description
Codon bias and its effect on protein production was studied using the model organism Drosophila melanogaster. Seven preferred leucine codons in the alcohol dehydrogenase (Adh) gene were changed to unpreferred codons. This gene (7-Up) was microinjected into a line of flies to create mutants carrying the mutant gene. Total soluble protein was isolated and assayed for total protein present and activity level of the enzyme. Ten control lines with the wildtype Adh gene and twelve mutant lines with the 7-Up gene showed average activity levels of 112.8 +/- 28.8 and 124.1 +/- 57.3 mumol NAD+ reduced per minute per mug of protein x 100, respectively. These results indicate that increasing codon bias artificially has no effect on protein production, possibly due to saturated tRNA pools in the cell.
Author: Jacob Lincoln Mueller
Publisher:
ISBN:
Category :
Languages : en
Pages : 560
Get Book Here
Book Description
Author: Ronny C. Woodruff
Publisher: Springer Science & Business Media
ISBN: 9401152101
Category : Science
Languages : en
Pages : 561
Get Book Here
Book Description
Although debated since the time of Darwin, the evolutionary role of mutation is still controversial. In over 40 chapters from leading authorities in mutation and evolutionary biology, this book takes a new look at both the theoretical and experimental measurement and significance of new mutation. Deleterious, nearly neutral, beneficial, and polygenic mutations are considered in their effects on fitness, life history traits, and the composition of the gene pool. Mutation is a phenomenon that draws attention from many different disciplines. Thus, the extensive reviews of the literature will be valuable both to established researchers and to those just beginning to study this field. Through up-to-date reviews, the authors provide an insightful overview of each topic and then share their newest ideas and explore controversial aspects of mutation and the evolutionary process. From topics like gonadal mosaicism and mutation clusters to adaptive mutagenesis, mutation in cell organelles, and the level and distribution of DNA molecular changes, the foundation is set for continuing the debate about the role of mutation, fitness, and adaptability. It is a debate that will have profound consequences for our understanding of evolution.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1844
Get Book Here
Book Description
Author: B. Schierwater
Publisher: Birkhäuser
ISBN: 3034875274
Category : Science
Languages : en
Pages : 615
Get Book Here
Book Description
The past 25 years have witnessed a revolution in the way ecologists and evolutionary biologists approach their disciplines. Modern molecular techniques are now reshaping the spectrum of questions that can be addressed while studying the mechanisms and consequences of the ecology and evolution of living organisms. "Molecular Ecology and Evolution: Approaches and Applications" describes, from a molecular perspective, several methodological and technical approaches used in the fields of ecology, evolution, population biology, molecular systematics, conservation genetics, and development. Modern techniques are introduced, and older, more classic ones refined. The advantages, limitations, and potentials of each are discussed in detail, and thereby illustrate the widening range of cross-field research and applications which this modern technology is stimulating. This book will serve as an important textbook for graduate and advanced undergraduate students, and as a key reference work for researchers
Author:
Publisher: Elsevier
ISBN: 0080858511
Category : Science
Languages : en
Pages : 415
Get Book Here
Book Description
Biological Roles and Function of Modification
Author: Friedrich K. Zimmermann
Publisher: CRC Press
ISBN: 9781566764667
Category : Technology & Engineering
Languages : en
Pages : 590
Get Book Here
Book Description
Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.
Author:
Publisher:
ISBN:
Category : Genetics
Languages : en
Pages : 844
Get Book Here
Book Description
Author: Julien Y Dutheil
Publisher:
ISBN: 9781013271403
Category : Science
Languages : en
Pages : 464
Get Book Here
Book Description
This open access volume presents state-of-the-art inference methods in population genomics, focusing on data analysis based on rigorous statistical techniques. After introducing general concepts related to the biology of genomes and their evolution, the book covers state-of-the-art methods for the analysis of genomes in populations, including demography inference, population structure analysis and detection of selection, using both model-based inference and simulation procedures. Last but not least, it offers an overview of the current knowledge acquired by applying such methods to a large variety of eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, pointers to the relevant literature, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Statistical Population Genomics aims to promote and ensure successful applications of population genomic methods to an increasing number of model systems and biological questions. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.
Author: Klaus Urich
Publisher: Springer Science & Business Media
ISBN: 3662063034
Category : Science
Languages : en
Pages : 798
Get Book Here
Book Description
tribute greatly to understanding the origins of The plan for this book goes back almost 20 years. Already, at that time, it was possible to recognize organisms. an extraordinary variation in metabolites and To provide the biochemist with a ready over processes superimposed upon the basic biochem view of the structural diversity of animals, the book includes a simplified version of animal sys ical system of animals. Each species, each indi tematics; for further information on the classifica vidual, in fact each type of cell of the multicellu lar organism possesses its own biochemical char tion, structure and life of particular animal spe acter, and this molecular variety, its biological sig cies, the reader should consult the relevant text nificance, and its evolutionary development books. It is assumed that the zoologist reader has throw up many interesting questions. The com a basic knowledge of biochemistry; important general biochemical facts are in any case given for parative approach that has been so productive at many of the subjects covered. the higher levels of complexity of morphology and physiology can also be used to great effect at I had already completed several chapters of the molecular level. this book by the beginning of the 1970s.
