Author: Ewa M. Goldys
Publisher: John Wiley & Sons
ISBN: 0470083700
Category : Science
Languages : en
Pages : 398
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Book Description
A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
Author: Bernard Valeur
Publisher: Springer Science & Business Media
ISBN: 364256853X
Category : Science
Languages : en
Pages : 496
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Book Description
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Author: Chris D. Geddes
Publisher: John Wiley & Sons
ISBN: 0470642785
Category : Science
Languages : en
Pages : 655
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Book Description
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Author: Bernard Valeur
Publisher: John Wiley & Sons
ISBN: 3527328467
Category : Science
Languages : en
Pages : 595
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Book Description
This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 0387463127
Category : Science
Languages : en
Pages : 961
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Book Description
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
Author: Ritesh K Shukla
Publisher: CRC Press
ISBN: 1000600149
Category : Science
Languages : en
Pages : 367
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Book Description
Forensic Microscopy: Truth Under the Lenses provides an overview and understanding of the various types of microscopes and their techniques employed in forensic science. The book emphasizes both the theoretical and practical aspects of microscopy to enrich the reader’s understanding of the various tools, techniques, and utility—including strengths and weaknesses—of types of microscopes in analyzing certain forms of evidence. The book begins with the history of microscopes, the basic optics for microscopy, then moves to advanced microscopies such as electron microscopes and atomic force microscopes. In addition to the various types of microscopes and how to use and best utilize them, the book looks at the analysis of specific types of evidence, including hair, fiber, fingerprint, body fluids, tool marks, ink, pollen grains, spores, diatoms, bullets, cartridges, among other evidence types. Since forensic science is an applied, hands-on discipline, the book includes both a theoretical and a practical approach to the topic. Key Features: Addresses simple to advanced microscopy techniques for the effective analyses of trace evidence Pairs chapters on a particular type of microscopy, explaining it thoroughly, before delving into specific usage for forensic applications Presents theories and as well as real-world application of concepts Provides abundant micro-photographs, including graphical representations and flow charts, to illustrate concepts clearly Forensic Microscopy serves as a helpful reference for undergraduate and postgraduate students in forensic science, forensic biology, forensic chemistry and related programs. It is also recommended for research students, academicians, technicians, industry and laboratory professionals working on trace evidence analysis.
Author: David J. Suggett
Publisher: Springer Science & Business Media
ISBN: 9048192684
Category : Science
Languages : en
Pages : 332
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Book Description
Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.
Author: Judith A.K. Howard
Publisher: Springer
ISBN: 9401785503
Category : Science
Languages : en
Pages : 292
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Book Description
This work focuses on complementary crystallographic and spectroscopic areas of dynamic structural science, from papers presented at the 46th NATO sponsored course in Erice, Sicily 2013. These papers cover a range of material from background concepts to more advanced material and represent a fully inter-disciplinary collection of the latest ideas and results within the field. They will appeal to practising or novice crystallographers, both chemical and biological, who wish to learn more about modern spectroscopic methods and convergent advances and hence vice versa for experimental and computational spectroscopists. The chapters refer to the latest techniques, software and results and each chapter is fully referenced. The volume provides an excellent starting point for new comers in the emerging, multi-disciplinary area of time resolved science.
Author: Prashant Kumar Singh
Publisher: Academic Press
ISBN: 0323910491
Category : Science
Languages : en
Pages : 384
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Book Description
Environmental change is affecting the world's agricultural productivity. This is coupled with an increase in population: according to the United Nations Department for Economic and Social Affairs, the global population is estimated to reach 9.7 billion by 2050. Therefore, the current situation requires that we develop climate-smart technologies to improve crop productivity to sustain the ever-rising global population. Current-day farmers are introducing a considerable amount of agrochemicals to enhance crop productivity. Indiscriminate agrochemical application has altered not only the soil's physic-chemical and biological properties but also affected human health through food chain contamination. Cyanobacteria, under these changing environmental conditions, may help to resolve the problem significantly without changing the natural soil properties. In spite of their well-known stress tolerance potential, most of the cyanobacterial stress management and signaling pathways are yet to be fully characterized. Therefore, there is an urgent need to explore cyanobacterial metabolism under stress as well as their regulatory pathways to exploit them for sustainable agriculture. In recent decades, the application of cyanobacteria has attracted scientists because of uniqueness, better adaptability, and synthetic products. Diverse cyanobacterial communities with the ability to fix atmospheric nitrogen, together with their photosynthetic properties, have demonstrated their application under field conditions. Several cyanobacterial species have thus been exploited to enhance soil fertility, mitigate biotic and abiotic stress, and contamination management. Cyanobacterial Lifestyle and its Applications in Biotechnology has been designed to discuss different aspects of cyanobacterial physiology with the aim of helping to provide a better understanding of advanced cyanobacterial molecular biology and their metabolism to uncover the potential of cyanobacteria in the tailoring of stress smart crops for sustainable agriculture. Chapters include valuable information about the role of cyanobacteria in the evolution of life, cyanobacterial photosynthesis, stress-tolerant cyanobacterium, biological nitrogen fixation, circadian rhythms, genetics and molecular biology of abiotic stress responses. - Summarizes various aspects of cyanobacterial research. - Includes comprehensive coverage of molecular approaches for the identification of cyanobacteria and their evolution. - Identifies an expanding horizon of cyanobacterial lifestyle: stress management in cyanobacteria. - Examines cyanobacteria synthetic biology, genetic engineering, photosynthesis and metabolic engineering.
Author: Gregor Jung
Publisher: Springer Science & Business Media
ISBN: 3642233724
Category : Science
Languages : en
Pages : 275
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Book Description
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins I is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.
