Author: S. Kim R. Williams
Publisher: Springer Science & Business Media
ISBN: 3709101530
Category : Science
Languages : en
Pages : 306
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Book Description
This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.
Author: S. Kim R. Williams
Publisher: Springer Science & Business Media
ISBN: 3709101549
Category : Science
Languages : en
Pages : 306
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Book Description
This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.
Author: Martin E. Schimpf
Publisher: John Wiley & Sons
ISBN: 9780471184300
Category : Science
Languages : en
Pages : 618
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Book Description
Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.
Author: Josef Janča
Publisher:
ISBN: 9780972806152
Category : Field-flow fractionation
Languages : en
Pages : 0
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Book Description
Microthermal field-flow fractionation (micro-TFFF) is a new method of polymer and particle separation and analysis based on the effect of thermal diffusion (Ludwig-Soret effect); the relationships between molecular characteristics of the separated macromolecular and particulate species such as molar mass, particle size, chemical nature, and composition; and their distribution and separation characteristics such as retention. The book begins with a description of the theoretical principles of FFF, which apply equally to micro-TFFF, although the latter technique offers a number of important operational advantages, including superior cold-wall temperature regulation. Practical analytical aspects of experimental data analysis are discussed in detail. Typical methodological applications of micro-TFFF are given for polymers and particles of synthetic, natural, or biological origin. The book will be of interest to university and R&D scientists involved in the analysis and characterization of polymers, biopolymers, particles, and biological cells in polymer chemistry, colloid and interface science, microbiology, and the environmental sciences.
Author: Martin Masuelli
Publisher: Bentham Science Publishers
ISBN: 1681084538
Category : Technology & Engineering
Languages : en
Pages : 542
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Book Description
The last two decades have seen a number of significant advances in the methodology for evaluating the molecular weight distributions of polydispersed macromolecular systems in solution at the molecular level. This reference presents reviews on the progress in different analytical and characterization methods of biopolymers. Readers will find useful information about combinations of complex biopolymer analysis such as chromatographic or membrane based fractionation procedures combined with multiple detectors on line (multi-angle laser light scattering or MALLS). Key topics include: • refractive index, UV-Vis absorbance and intrinsic viscosity detection systems, • advances in SEC-MALLS (size exclusion chromatography coupled to multi-angle laser light scattering) and FFF-MALLS (field flow fractionation coupled on line to MALLS), • HPSEC-A4F-MALLS, matrix-assisted laser-desorption ionization (MALDI) • electrospray ionization (ESI) mass spectrometry • nuclear magnetic resonance (NMR) spectroscopy This reference is intended for students of applied chemistry and biochemistry who require information about biopolymer analysis and characterization.
Author: Josef Janca
Publisher: CRC Press
ISBN: 1000715566
Category : Science
Languages : en
Pages : 349
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Book Description
Field-flow fractionation has become a very effective method for the separation and analytical characterization of substances of macromolecular and particulate character-making this method probably the most important discovery in the analytical separation field since World War II. The first, complete, up-to-date reference on this notable separation method, Field-Flow Fractionation includes comprehensive explanations of general and theoretical principles . . . presents detailed descriptions of experimental techniques and instrumentation ... and discusses advantages over competitive processes. In addition, this resource demonstrates various applications for characterizing synthetic polymers ... analyzing biopolymers and particles in environmental samples ... and determining sizes of biological cells, viruses, and subcellular particles. Substantiating main conclusions of theories, experiments, and applications with graphs and drawings, and including numerous tables and photographs, this one-source reference is invaluable reading for analytical, industrial, physical, and polymer chemists; chrom atographers; biochemists; biotechnologists; biophysicists; and all others interested in the study and characterization of macromolecules or particles of various origins.
Author: Josef Janca
Publisher: CRC Press
ISBN: 1000723380
Category : Science
Languages : en
Pages : 358
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Book Description
Field-flow fractionation has become a very effective method for the separation and analytical characterization of substances of macromolecular and particulate character-making this method probably the most important discovery in the analytical separation field since World War II. The first, complete, up-to-date reference on this notable separation method, Field-Flow Fractionation includes comprehensive explanations of general and theoretical principles . . . presents detailed descriptions of experimental techniques and instrumentation ... and discusses advantages over competitive processes. In addition, this resource demonstrates various applications for characterizing synthetic polymers ... analyzing biopolymers and particles in environmental samples ... and determining sizes of biological cells, viruses, and subcellular particles. Substantiating main conclusions of theories, experiments, and applications with graphs and drawings, and including numerous tables and photographs, this one-source reference is invaluable reading for analytical, industrial, physical, and polymer chemists; chrom atographers; biochemists; biotechnologists; biophysicists; and all others interested in the study and characterization of macromolecules or particles of various origins.
Author: Stepan Podzimek
Publisher: John Wiley & Sons
ISBN: 111810272X
Category : Technology & Engineering
Languages : en
Pages : 325
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Book Description
A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers—light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to deliver a complete and thorough explanation of the principles, theories, and instrumentation needed to characterize polymers from the viewpoint of their molar mass distribution, size, branching, and aggregation. This comprehensive resource: Is the only book gathering light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation into a single text Systematically compares results of size exclusion chromatography with results of asymmetric flow field flow fractionation, and how these two methods complement each other Provides in-depth guidelines for reproducible and correct determination of molar mass and molecular size of polymers using SEC or A4F coupled with a multi-angle light scattering detector Offers a detailed overview of the methodology, detection, and characterization of polymer branching Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation should be of great interest to all those engaged in the polymer analysis and characterization in industrial and university research, as well as in manufacturing quality control laboratories. Both beginners and experienced can confidently rely on this volume to confirm their own understanding or to help interpret their results.
Author: Muhammad Imran Malik
Publisher: Elsevier
ISBN: 012822598X
Category : Technology & Engineering
Languages : en
Pages : 670
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Book Description
Molecular Characterization of Polymers presents a range of advanced and cutting-edge methods for the characterization of polymers at the molecular level, guiding the reader through theory, fundamentals, instrumentation, and applications, and supporting the end goal of efficient material selection and improved material performance. Each chapter focuses on a specific technique or family of techniques, including the different areas of chromatography, field flow fractionation, long chain branching, static and dynamic light scattering, mass spectrometry, NMR, X-Ray and neutron scattering, polymer dilute solution viscometry, microscopy, and vibrational spectroscopy. In each case, in-depth coverage explains how to successfully implement and utilize the technique. This practical resource is highly valuable to researchers and advanced students in polymer science, materials science, and engineering, and to those from other disciplines and industries who are unfamiliar with polymer characterization techniques. Introduces a range of advanced characterization methods, covering aspects such as molecular weight, polydispersity, branching, composition, and tacticity Enables the reader to understand and to compare the available technique, and implement the selected technique(s), with a view to improving properties of the polymeric material Establishes a strong link between basic principles, characterization techniques, and real-life applications
Author: Patrick J. Sinko
Publisher: Lippincott Williams & Wilkins
ISBN: 1975174828
Category : Medical
Languages : en
Pages : 1857
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Book Description
Consistently revised and updated for more than 60 years to reflect the most current research and practice, Martin’s Physical Pharmacy and Pharmaceutical Sciences, 8th Edition, is the original and most comprehensive text available on the physical, chemical, and biological principles that underlie pharmacology and the pharmaceutical sciences. An ideal resource for PharmD and pharmacy students worldwide, teachers, researchers, or industrial pharmaceutical scientists, this 8th Edition has been thoroughly revised, enhanced, and reorganized to provide readers with a clear, consistent learning experience that puts essential principles and concepts in a practical, approachable context. Updated content reflects the latest developments and perspectives across the full spectrum of physical pharmacy and a new full-color design makes it easier than ever to discover, distinguish, and understand information—providing users the most robust support available for applying the elements of biology, physics, and chemistry in work or study.
