Experiments with Gene Fusions

Experiments with Gene Fusions PDF Author: Thomas J. Silhavy
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 332

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Book Description

Experiments with Gene Fusions

Experiments with Gene Fusions PDF Author: Thomas J. Silhavy
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 332

Get Book Here

Book Description


Experiments with Gene Fusions

Experiments with Gene Fusions PDF Author: Silharvy, Thomas J.
Publisher:
ISBN:
Category :
Languages : en
Pages : 303

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Book Description


Construction of Gene Fusions for Testing Intracellular Sorting Signals of Plant Seed Proteins

Construction of Gene Fusions for Testing Intracellular Sorting Signals of Plant Seed Proteins PDF Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 172

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Book Description


Green Fluorescent Protein

Green Fluorescent Protein PDF Author: Martin Chalfie
Publisher: John Wiley & Sons
ISBN: 0471739480
Category : Science
Languages : en
Pages : 486

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Book Description
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Experiments in Molecular Genetics

Experiments in Molecular Genetics PDF Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 492

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Book Description


Construction of gene fusions for testing intracellular sorting signals of plant need proteins

Construction of gene fusions for testing intracellular sorting signals of plant need proteins PDF Author: Nong Van Hai
Publisher:
ISBN:
Category :
Languages : de
Pages : 172

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Book Description


Guide to Protein Purification

Guide to Protein Purification PDF Author: Murray P. Deutscher
Publisher: Gulf Professional Publishing
ISBN: 9780121820831
Category : Proteins
Languages : en
Pages : 936

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Book Description
Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.

Experimental Manipulation of Gene Expression

Experimental Manipulation of Gene Expression PDF Author: Masayori Inouye
Publisher: Academic Press
ISBN: 1483273970
Category : Science
Languages : en
Pages : 330

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Book Description
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.

Safety of Genetically Engineered Foods

Safety of Genetically Engineered Foods PDF Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254

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Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

RNA-Based Regulation in Human Health and Disease

RNA-Based Regulation in Human Health and Disease PDF Author:
Publisher: Academic Press
ISBN: 0128171944
Category : Medical
Languages : en
Pages : 444

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Book Description
RNA-based Regulation in Human Health and Disease offers an in-depth exploration of RNA mediated genome regulation at different hierarchies. Beginning with multitude of canonical and non-canonical RNA populations, especially noncoding RNA in human physiology and evolution, further sections examine the various classes of RNAs (from small to large noncoding and extracellular RNAs), functional categories of RNA regulation (RNA-binding proteins, alternative splicing, RNA editing, antisense transcripts and RNA G-quadruplexes), dynamic aspects of RNA regulation modulating physiological homeostasis (aging), role of RNA beyond humans, tools and technologies for RNA research (wet lab and computational) and future prospects for RNA-based diagnostics and therapeutics. One of the core strengths of the book includes spectrum of disease-specific chapters from experts in the field highlighting RNA-based regulation in metabolic & neurodegenerative disorders, cancer, inflammatory disease, viral and bacterial infections. We hope the book helps researchers, students and clinicians appreciate the role of RNA-based regulation in genome regulation, aiding the development of useful biomarkers for prognosis, diagnosis, and novel RNA-based therapeutics. Comprehensive information of non-canonical RNA-based genome regulation modulating human health and disease Defines RNA classes with special emphasis on unexplored world of noncoding RNA at different hierarchies Disease specific role of RNA - causal, prognostic, diagnostic and therapeutic Features contributions from leading experts in the field