Experimental Study of Laser Desorbed Biological Molecules Using Ion-trap/reflectron Time-of-flight Mass Spectrometry PDF Download
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Author: Heewon Lee
Publisher:
ISBN:
Category :
Languages : en
Pages : 462
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Author: Heewon Lee
Publisher:
ISBN:
Category :
Languages : en
Pages : 462
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Book Description
Author: Nicolas C. Polfer
Publisher: Springer Science & Business Media
ISBN: 3319012525
Category : Science
Languages : en
Pages : 128
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Book Description
This lecture notes book presents how enhanced structural information of biomolecular ions can be obtained from interaction with photons of specific frequency - laser light. The methods described in the book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" make use of the fact that the discrete energy and fast time scale of photoexcitation can provide more control in ion activation. This activation is the crucial process producing structure-informative product ions that cannot be generated with more conventional heating methods, such as collisional activation. The book describes how the powerful separation capabilities and sensitivity of mass spectrometry (MS) can be combined with the structural insights from spectroscopy by measuring vibrational and electronic spectra of trapped analytes. The implementation of laser-based photodissociation techniques in MS requires basic knowledge of tunable light sources and ion trapping devices. This book introduces the reader to key concepts and approaches in molecular spectroscopy, and the light sources and ion traps employed in such experiments. The power of the methods is demonstrated by spectroscopic interrogation of a range of important biomolecular systems, including peptides, proteins, and saccharides, with laser light in the ultraviolet-visible, and infrared range. The book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" is an indispensable resource for students and researchers engaged or interested in this emerging field. It provides the solid background of key concepts and technologies for the measurements, discusses state-of-the-art experiments, and provides an outlook on future developments and applications.
Author: Julie A. Lloyd
Publisher:
ISBN:
Category : Aerosols
Languages : en
Pages :
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Book Description
Utilizing the fundamental principles of analytical chemistry such as controlling measurement variables and developing instrumentation is essential to understanding the complexity of the atmosphere. The work in this thesis will focus on conducting controlled laboratory experiments with ozone and particulate matter as well as the development of a new mass spectrometer for organic aerosol analysis. Angiotensin II (DRV Y I H P F) and two analogs (DRV Y I A P A, and DRV A I H P A) were used as model systems to study the ozonolysis of peptides containing tyrosine and histidine residues. New oxidation products, Tyr + 3O and Tyr + 34 Da, were observed as well as previously proposed products, Tyr + O, His + 3O, and Phe + O. Second order rate constants were calculated, and the relative reactivity of the isolated His residue was slightly greater than an isolated Tyr residue. Lifetimes of the peptides in the atmosphere were estimated to be on the order of 20-30 days assuming no other decomposition pathways. A novel IT-TOF mass spectrometer with the ability to perform multiple ionization techniques such as LDI, LD-EI, and MALDI was designed and applied to the online analysis of organic aerosol. Airborne particles are first focused through an aerodynamic lens assembly and deposited onto a stainless steel collection probe inserted through a hole in the ring electrode of an ion trap. The collected sample is desorbed and ionized with a pulsed 355 or 1064 nm Nd:YAG laser beam within the ion trap cavity. Ions are collisionally cooled to the center of the trap prior to extraction into a reflectron time-of-flight mass analyzer. Parameters such as RF amplitude, laser energies, timing delays, and probe materials were investigated. The IT-TOF mass spectrometer was applied to study the reaction of ammonium nitrate particles with trimethylamine (TMA) vapor. When exposed to a large excess of TMA, complete exchange of ammonia by TMA was observed. Lower TMA concentrations resulted in partial exchange. The reactive uptake coefficient was estimated to be 2 x 10 -6, but this is likely to be a lower limit due to losses of TMA prior to introduction into the reaction system.
Author: Yan-Hui Liu
Publisher:
ISBN:
Category :
Languages : en
Pages : 460
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Author: Yongdong Zhu
Publisher:
ISBN:
Category :
Languages : en
Pages : 364
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Book Description
Author: Chava Lifshitz
Publisher: John Wiley & Sons
ISBN: 0470050411
Category : Science
Languages : en
Pages : 707
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Book Description
An extensive compilation of articles by leading professionals, this reference explains the fundamental principles of mass spectrometry as they relate to the life sciences. Topics covered include spectroscopy, energetics and mechanisms of peptide fragmentation, electron capture dissociation, ion-ion and ion-molecule reactions, reaction dynamics, collisional activation, soft-landing, protein structure and interactions, thermochemistry, and more. The book empowers readers to develop new ways of using these techniques.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 22
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Book Description
Recent efforts to detect and identify large biomolecules using matrix assisted laser desorption have shown MALDI to be an extremely sensitive technique for trace detection from a solid sample. In the DI process, ions are formed by proton transfer from an electronically excited organic acid to a suitable proton accepting analyte. However, detection and characterization of biomolecules may benefit from ionization of the isolated unprotonated gas phase neutrals. To this end, a custom time-of-flight mass spectrometer as been constructed to perform two-laser matrix assisted laser desorption/laser ionization studies using separate desorption and ionization lasers. The two chamber, differentially pumped apparatus incorporates numerous laser and sample probe entry ports, a pulsed valve, and a modified Wiley-NcLaren mass spectrometer design. Results of initial two-laser experiments on phenylalanine in a sinnapinic acid matrix have demonstrated the apparatus' ability to detect laser ionization of laser desorbed neutrals.
Author: Teresa Catherine Cain
Publisher:
ISBN:
Category :
Languages : en
Pages : 234
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Book Description
Author: A.L. Burlingame
Publisher: Gulf Professional Publishing
ISBN: 9780121828073
Category : Science
Languages : en
Pages : 520
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Book Description
Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.
Author: James Christopher Reynolds
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
