Experimental Manipulation of Gene Expression PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Experimental Manipulation of Gene Expression PDF full book. Access full book title Experimental Manipulation of Gene Expression by Masayori Inouye. Download full books in PDF and EPUB format.
Author: Masayori Inouye
Publisher: Academic Press
ISBN: 1483273970
Category : Science
Languages : en
Pages : 330
Get Book
Book Description
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
Author: Masayori Inouye
Publisher: Academic Press
ISBN: 1483273970
Category : Science
Languages : en
Pages : 330
Get Book
Book Description
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
Author: S. J. Ennion
Publisher: Cambridge University Press
ISBN: 9780521570039
Category : Nature
Languages : en
Pages : 232
Get Book
Book Description
The techniques of molecular biology offer a powerful means of investigating and controlling the genetic basis of mechanisms operating in living organisms. The development of these techniques in aquatic animals has now reached the stage where important questions relating to growth, development and adaptation to the environment can be addressed at the level of gene expression, and the introduction and expression of novel genes achieved. This volume presents some of the most exciting advances in this rapidly expanding area, with contributions on the evolution of adaptation to low temperature, adaptation to short-term fluctuations in temperature and salinity, gene expression during growth and development, myosin polymorphism and the generation of transgenic fish. As such, it will be of interest to all those working in the fields of marine and freshwater biology and also to those working in aquaculture.
Author: Sandy B. Primrose
Publisher: John Wiley & Sons
ISBN: 1405135441
Category : Science
Languages : en
Pages : 675
Get Book
Book Description
The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics. Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics Includes two new chapters on the applications of genomics An accompanying website - www.blackwellpublishing.com/primrose - provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format. An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254
Get Book
Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
Get Book
Book Description
Author: Richard W Van Norman
Publisher: Legare Street Press
ISBN: 9781022890046
Category :
Languages : en
Pages : 0
Get Book
Book Description
This comprehensive textbook covers the basic principles and techniques of experimental biology, from basic cell biology to the intricacies of genetic manipulation and gene expression. Richard W Van Norman provides a thorough and engaging introduction to the field, incorporating real-world examples and case studies to help readers deepen their understanding of how biological research is conducted. This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work is in the "public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309035422
Category : Technology & Engineering
Languages : en
Pages : 136
Get Book
Book Description
Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309034345
Category : Science
Languages : en
Pages : 97
Get Book
Book Description
"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."
Author: Paulina Balbas
Publisher: Springer Science & Business Media
ISBN: 1592597742
Category : Science
Languages : en
Pages : 505
Get Book
Book Description
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Author: Christopher Howe
Publisher: Cambridge University Press
ISBN: 1139463586
Category : Medical
Languages : en
Pages : 250
Get Book
Book Description
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
