Engineering Zymomonas Mobilis for the Production of Biofuels and Other Value-added Products PDF Download
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Languages : en
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Author:
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Languages : en
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Author: Guillermo Gosset
Publisher: Springer
ISBN: 3319517295
Category : Medical
Languages : en
Pages : 203
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Book Description
This book reviews state of the art regarding strategies for generating and improving microbial strains designed for utilizing renewable raw materials. It discusses methods for genetically engineering of thermophilic bacteria, Saccharomyces cerevisiae, Escherichia coli and Zymomonas mobilis, as well as approaches for obtaining useful products from these renewable raw materials based on biotechnological processes using microbes to chemically transform them. However, the efficient transformation of lignocellulosic biomass or glycerol to useful products represents a major challenge: Biomass has to be treated physically and chemically to release a mixture of sugars that potentially can be employed by the microbial production strains. These hydrolytic treatments result in diverse toxic compounds being generated and released, that negatively impact strain performance. Furthermore, most of the commonly used industrial microbes do not have the natural capacity to efficiently utilize and transform the generated sugar mixtures or glycerol. The microbial species reviewed in this book possess particular advantages as production strains and are currently employed for the synthesis of numerous biofuels and chemicals. The book reviews the general and strain-specific genetic engineering strategies for the improvement of sugar mixtures and glycerol catabolism. The issue of lignocellulosic hydrolysate toxicity is addressed in several chapters, where genetic engineering and adaptive laboratory evolution strategies are reviewed and discussed. The objective of this book is to provide the current knowledge regarding strategies for the generation and improvement of microbial strains designed for the transformation of renewable raw materials into useful products. This book aims to become a reference for researchers and students working in this field.
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Languages : en
Pages : 15
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Book Description
To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen Zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-BDO). 2,3-BDO is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. 2,3-BDO biosynthesis pathways from various bacterial species were examined, which include three genes encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase. Bioinformatics analysis was carried out to pinpoint potential bottlenecks for high 2,3-BDO production. Different combinations of 2,3-BDO biosynthesis metabolic pathways using genes from different bacterial species have been constructed. Our results demonstrated that carbon flux can be deviated from ethanol production into 2,3-BDO biosynthesis, and all three heterologous genes are essential to efficiently redirect pyruvate from ethanol production for high 2,3-BDO production in Z. mobilis. The down-selection of best gene combinations up to now enabled Z. mobilis to reach the 2,3-BDO production of more than 10 g/L from glucose and xylose, as well as mixed C6/C5 sugar streams derived from the deacetylation and mechanical refining process. In conclusion, this study confirms the value of integrating bioinformatics analysis and systems biology data during metabolic engineering endeavors, provides guidance for value-added chemical production in Z. mobilis, and reveals the interactions between host metabolism, oxygen levels, and a heterologous 2,3-BDO biosynthesis pathway. Taken together, this work provides guidance for future metabolic engineering efforts aimed at boosting 2,3-BDO titer anaerobically.
Author: Rajesh K. Sani
Publisher: Springer
ISBN: 3319744593
Category : Science
Languages : en
Pages : 313
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Book Description
This book presents a review and in-depth analyses of improved biotechnological processes emphasizing critical aspects and challenges of lignocellulosic biomass conversion into biofuels and value-added products especially using extremophiles and recombinant microorganisms. The book specifically comprises extremophilic production of liquid and gaseous biofuels (bioethanol, biobutanol, biodiesel, biohydrogen, and biogas) as well as value added products (e.g. single cell protein, hydrocarbons, lipids, exopolysaccharides, and polyhydroxyalkanoates). The book also provides the knowledge on how to develop safe, more efficient, sustainable, and economical integrated processes for enhanced conversion of lignocellulosic feedstocks to liquid and gaseous biofuels. Finally the book describes how to perform the techno-economical and life-cycle assessments of new integrated processes involving extremophiles. These modeling exercises are critical in addressing any deficiencies associated with the demonstration of an integrated biofuels and value-added products production process at pilot scale as well as demonstration on the commercialization scale.
Author: Shang-Tian Yang
Publisher: Elsevier
ISBN: 0080466710
Category : Technology & Engineering
Languages : en
Pages : 685
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Book Description
Bioprocessing for Value-Added Products from Renewable Resources provides a timely review of new and unconventional techniques for manufacturing high-value products based on simple biological material. The book discusses the principles underpinning modern industrial biotechnology and describes a unique collection of novel bioprocesses for a sustainable future. This book begins in a very structured way. It first looks at the modern technologies that form the basis for creating a bio-based industry before describing the various organisms that are suitable for bioprocessing - from bacteria to algae - as well as their unique characteristics. This is followed by a discussion of novel, experimental bioprocesses, such as the production of medicinal chemicals, the production of chiral compounds and the design of biofuel cells. The book concludes with examples where biological, renewable resources become an important feedstock for large-scale industrial production. This book is suitable for researchers, practitioners, students, and consultants in the bioprocess and biotechnology fields, and for others who are interested in biotechnology, engineering, industrial microbiology and chemical engineering. ·Reviews the principles underpinning modern industrial biotechnology ·Provides a unique collection of novel bioprocesses for a sustainable future ·Gives examples of economical use of renewable resources as feedstocks ·Suitable for both non-experts and experts in the bioproduct industry
Author: Preshanthan Moodley
Publisher: Woodhead Publishing
ISBN: 032391344X
Category : Science
Languages : en
Pages : 438
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Book Description
Advances in Lignocellulosic Biofuel Production Systems focuses on general topics such as novel pretreatment strategies, lignocellulosic biomass as a suitable feedstock for biofuels, lifecycle assessment and integrated biorefineries. Furthermore, the book focuses on more advanced topics such as genetically engineered feedstocks, metabolically engineered microbes, bioreactor design and configuration, cell immobilization strategies, artificial intelligence applications and nanotechnology. This book will guide readers through all aspects of lignocellulosic biofuel production rather than simply covering a single topic. - Provides information on the most advanced and innovative technologies for biomass valorization, including the design and configuration of bioreactors - Identifies research gaps in the application of artificial intelligence, nanotechnology, cell immobilization, metabolic engineering, kinetic assessment and genetically engineered feedstocks for enhancing lignocellulosic bioprocessing and biofuel yield - Presents a global overview of the supply chain for biofuels production from lignocellulosic biomass - Includes techno-economic analysis, along with environmental and socioeconomic impact assessments of various technologies
Author: Vikash Babu
Publisher: John Wiley & Sons
ISBN: 1118835735
Category : Technology & Engineering
Languages : en
Pages : 248
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Book Description
The search for alternative sources of energy to offset diminishing resources of easy and cost-effective fossil fuels has become a global initiative, and fuel generated from biomass is a leading competitor in this arena. Large-scale introduction of biofuels into the energy mix could contribute to environmentally and economicaly sustainable development on a global scale. The processes and methodologies presented in this volume will offer a cutting-edge and comprehensive approach to the production of biofuels, for engineers, researchers, and students.
Author: Sunita Varjani
Publisher: Elsevier
ISBN: 0323910467
Category : Technology & Engineering
Languages : en
Pages : 674
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Book Description
Biomass, Biofuels, Biochemicals: Circular Bioeconomy: Technologies for Biofuels and Biochemicals provides comprehensive information on strategies and approaches that facilitate the integration of technologies for the production of bio-based fuels, chemicals and other value-added products from wastes with waste biorefinery concepts and green strategies. The book also covers lifecycle assessment and techno-economic analyses of integrated biorefineries within a circular bioeconomy framework. As there has been continual research on new designs in production and consumerist approaches as we move towards sustainable development by scientists of various disciplines, law makers, environmental activists and industrialists, this book provides the latest details. Resources consumption and environment degradation necessitates a transition of our linear economy towards sustainable social and technical systems. As fossil resources are only projected to fulfill the needs of the population for the next couple of centuries, new tactics and standards must be created to ensure future success. - Covers recent developments and perspectives on biofuels and chemicals production - Provides the latest on the integration of technologies and processes for biofuels and chemicals production - Paves a way forward roadmap to achieve Sustainable Development Goals - Covers recent developments in lifecycle assessment and techno economic analysis using a waste biorefinery approach
Author: Achlesh Daverey
Publisher: Elsevier
ISBN: 0323954502
Category : Technology & Engineering
Languages : en
Pages : 630
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Book Description
Advances in Yeast Biotechnology for Biofuels and Sustainability: Value-Added Products and Environmental Remediation Applications showcases the uses for engineered yeast in environmental applications, especially as an innovative source of biofuels. Beginning with a thorough review of recent advances and future potential for yeast biotechnology, the book proceeds to outline several options for biofuels, including lignocellulosic biofuels and alternative feedstock production through hydrolysis and alternative value-added products, including industrial acids and bioplastics and applications in agriculture and environmental remediation. Placing case studies at the center of each chapter, this book presents a future-focused perspective on the potential of yeast biotechnologies to support sustainability. - Lays out methods, including multiple options for generating biofuels from engineered yeast and several additional value-added products - Presents a wide variety of real-world sustainable applications for engineered yeast, with a focus on biofuels production - Provides a selection of case studies in other value-added products and applications, including bioremediation, pollution remediation, and biofertilizers in sustainable agriculture
Author: R. Navanietha Krishnaraj
Publisher:
ISBN: 0128179511
Category :
Languages : en
Pages : 422
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Book Description
Biovalorisation of Wastes to Renewable Chemicals and Biofuels addresses advanced technologies for converting waste to biofuels and value-added products. Biovalorisation has several advantages over conventional bioremediation processes as it helps reduce the costs of bioprocesses. Examples are provided of several successfully commercialized technologies, giving insight into developing, potential processes for biovalorisation of different wastes. Different bioprocess strategies are discussed for valorising the wastes coming from the leather industry, olive oil industry, pulp and paper, winery, textile, and food industries, as well as aquaculture. A section on biorefinery for hydrocarbons and emerging contaminants is included to cover concepts on biodesulfurization of petroleum wastes, leaching of heavy metals from E - waste, and bioelectrochemical processes for CO2. Chapters on algal biorefinery are also included to focus on the technologies for conversion of CO2 sequestration and wastewater utilization. Biovalorisation of Wastes to Renewable Chemicals and Biofuels can be used as course material for graduate students in chemical engineering, chemistry, and biotechnology, and as a reference for industrial professionals and researchers who want to gain a basic understanding on the subject. Covers a wide range of topics, from the conversion of wastes to organic acids, biofuels, biopolymers and industrially relevant products Bridges the gap between academics and industry Written in a lucid and self-explanatory style Includes activities/quiz/critical questions
