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Author: Anne H. Delcour
Publisher: Springer
ISBN: 3319201492
Category : Science
Languages : en
Pages : 387
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Book Description
This book is dedicated to the channels and pores that belong to an eclectic and ubiquitous class of unconventional - perhaps at times strange - pore-forming molecules, which nevertheless play fundamental roles in various organisms. These non-canonical channels may take on various and sometimes complex architectures, such as large beta-barrels or lipid-containing pores. They may originate from bacteria, viruses or intracellular organelles. For some of them, the physiologically relevant substrate may indeed be ions, and for others folded polypeptides. Some are released by cells in a soluble form that has the ability to insert into biological membranes to exert its permeabilizing effect. Many of these unconventional pores have been investigated by electrophysiology, which, by its virtue of focusing on a few or even a single unit, has provided invaluable insight into the mechanisms and structure-function relationships of these remarkable membrane entities. The chapters of this book highlight a representative set of these interesting investigations.
Author: Anne H. Delcour
Publisher: Springer
ISBN: 3319201492
Category : Science
Languages : en
Pages : 387
Get Book Here
Book Description
This book is dedicated to the channels and pores that belong to an eclectic and ubiquitous class of unconventional - perhaps at times strange - pore-forming molecules, which nevertheless play fundamental roles in various organisms. These non-canonical channels may take on various and sometimes complex architectures, such as large beta-barrels or lipid-containing pores. They may originate from bacteria, viruses or intracellular organelles. For some of them, the physiologically relevant substrate may indeed be ions, and for others folded polypeptides. Some are released by cells in a soluble form that has the ability to insert into biological membranes to exert its permeabilizing effect. Many of these unconventional pores have been investigated by electrophysiology, which, by its virtue of focusing on a few or even a single unit, has provided invaluable insight into the mechanisms and structure-function relationships of these remarkable membrane entities. The chapters of this book highlight a representative set of these interesting investigations.
Author: Anne H. Delcour
Publisher:
ISBN: 9783319201504
Category :
Languages : en
Pages :
Get Book Here
Book Description
This book is dedicated to the channels and pores that belong to an eclectic and ubiquitous class of unconventional - perhaps at times strange - pore-forming molecules, which nevertheless play fundamental roles in various organisms. These non-canonical channels may take on various and sometimes complex architectures, such as large beta-barrels or lipid-containing pores. They may originate from bacteria, viruses or intracellular organelles. For some of them, the physiologically relevant substrate may indeed be ions, and for others folded polypeptides. Some are released by cells in a soluble form that has the ability to insert into biological membranes to exert its permeabilizing effect. Many of these unconventional pores have been investigated by electrophysiology, which, by its virtue of focusing on a few or even a single unit, has provided invaluable insight into the mechanisms and structure-function relationships of these remarkable membrane entities. The chapters of this book highlight a representative set of these interesting investigations.
Author: Tatiana K. Rostovtseva
Publisher: Springer
ISBN: 3319555391
Category : Science
Languages : en
Pages : 388
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Book Description
This book covers recent advances in the study of structure, function, and regulation of metabolite, protein and ion translocating channels, and transporters in mitochondria. A wide array of cutting-edge methods are covered, ranging from electrophysiology and cell biology to bioinformatics, as well as structural, systems, and computational biology. At last, the molecular identity of two important channels in the mitochondrial inner membrane, the mitochondrial calcium uniporter and the mitochondrial permeability transition pore have been established. After years of work on the physiology and structure of VDAC channels in the mitochondrial outer membrane, there have been multiple discoveries on VDAC permeation and regulation by cytosolic proteins. Recent breakthroughs in structural studies of the mitochondrial cholesterol translocator reveal a set of novel unexpected features and provide essential clues for defining therapeutic strategies. Molecular Basis for Mitochondrial Signaling covers these and many more recent studies of mitochondria function, their communication with other organelles, and their critical roles in development, aging, and in a plethora of stressful or degenerative events. Authored by leading researchers in the field, this volume will be an indispensable reference resource for graduate students and academics working in related areas of biophysics and cell biology as well as for professionals within industry.
Author: Megha Agrawal
Publisher: BoD – Books on Demand
ISBN: 183968626X
Category : Medical
Languages : en
Pages : 646
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Book Description
This book provides an inclusive and comprehensive discussion of the transmission, science, biology, genome sequencing, diagnostics, and therapeutics of COVID-19. It also discusses public and government health measures and the roles of media as well as the impact of society on the ongoing efforts to combat the global pandemic. It addresses almost every topic that has been studied so far in the research on SARS-CoV-2 to gain insights into the fundamentals of the disease and mitigation strategies. This volume is a useful resource for virologists, epidemiologists, biologists, medical professionals, public health and government professionals, and all global citizens who have endured and battled against the pandemic.
Author: Madan L. Nagpal Ph.D. FLS.
Publisher: Xlibris Corporation
ISBN: 1669863905
Category : Medical
Languages : en
Pages : 427
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Book Description
COVID-19 outbreak has become a worldwide pandemic. This book “CO-VI-D: Viruses/Vaccines” provides new and unique approach to the discussion of viruses and vaccines in the recent advent of Corona virus. COVID-19 disease is a novel viral disease that was first identified in Wuhan, China. The World Health Organization (WHO) announced its official name, Coronavirus disease 2019, abbreviated as COVID-19; ‘CO’ stands for ‘Corona’, ‘VI’ stands for ‘Virus’, and ‘D’ stands for ‘Disease’. Globally, more than 10 million people are reported to be infected by COVID-19 (WHO Coronavirus Disease Dashboard, 2020). Centers for Disease Control and Prevention (CDC, USA, 2020) (provided guidelines on how to reduce the risk of infection. It has been discovered that some people get really very sick and die, whereas to some people nothing happens even though they test positive for the virus. Through this book, we intend to explore etiology, development, and treatment of the COVID-19 disease. The specific topics will give details that could help in understanding symptoms, immunity, worldwide spread and treatments so that we could beat this invisible dangerous beast, the COVID-19.
Author: Holger Barth
Publisher: MDPI
ISBN: 3038424307
Category : Science
Languages : en
Pages : 125
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Book Description
This book is a printed edition of the Special Issue "Novel Pharmacological Inhibitors for Bacterial Protein Toxins" that was published in Toxins
Author: Elena G. Govorunova
Publisher: MDPI
ISBN: 3039436732
Category : Science
Languages : en
Pages : 164
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Book Description
This book provides an update for the rapidly developing technology known as “optogenetics”, which is the use of genetically encoded light-sensitive molecular elements (usually derived from lower organisms) to control or report various physiological and biochemical processes within the cell. Two ongoing clinical trials use optogenetic tools for vision restoration, and optogenetic strategies have been suggested as novel therapies for several neurological, psychiatric and cardiac disorders. This Special Issue comprises two reviews and seven experimental papers on different types of light-sensitive modules widely used in optogenetic studies. These papers demonstrate the efficiency and versatility of optogenetics and are expected to be equally relevant for advanced users and beginners considering using optogenetic tools in their research.
Author: Shuk-Kwan Lee
Publisher: Open Dissertation Press
ISBN: 9781361038475
Category : Medical
Languages : en
Pages : 142
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Book Description
This dissertation, "Characterization of Two-pore Channel 2 by Nuclear Membrane Electrophysiology" by Shuk-kwan, Lee, 李淑君, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Characterization of Two-Pore Channel 2 by Nuclear Membrane Electrophysiology Submitted by Lee Shuk Kwan for the degree of Doctor of Philosophy at The University of Hong Kong in July 2016 2+ NAADP-mediated lysosomal calcium (Ca ) release induces signalling cascades that regulate various cellular processes. The discovery of two-pore channel 2 2+ (TPC2) as the NAADP receptor advances our understanding of lysosomal Ca signalling, yet the intracellular localization of lysosomes make them unamenable to traditional patch-clamp electrophysiology. Prior attempts to record TPC2 single-channel activity placed TPC2 outside its native environment and did not accurately reproduce the physiological properties of TPC2. To examine the feasibility of using nuclear membrane electrophysiology to characterize the TPC2 channel, we constructed a stable human TPC2-expressing DT40TKO cell line lacking endogenous InsP3Rs and RyRs (DT40TKO-hTPC2). Immunocytochemistry revealed hTPC2 expression on the ER and nuclear envelope. Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 2+ cells evoked cytosolic Ca transients, suggesting that the expressed hTPC2 was i functionally active. Utilizing nuclear membrane patch clamp electrophysiology, we detected a 220 pS single-channel current activated by NAADP with K as the permeant ion. The recorded single-channel currents displayed a linear current- voltage relationship, were sensitive to Ned-19 inhibition, were biphasically regulated by NAADP concentration, and were regulated by PKA phosphorylation. In conclusion, we developed a novel cell model for the characterization of the TPC2 channel, and nuclear membrane patch-clamp methodology provided an alternative approach to robustly investigate the electrophysiological properties of TPC2 with minimal manipulation. (Word count: 209) ii Subjects: Electrophysiology
Author: Shuk-Kwan Lee
Publisher:
ISBN: 9781361038499
Category :
Languages : en
Pages :
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Book Description
This dissertation, "Characterization of Two-pore Channel 2 by Nuclear Membrane Electrophysiology" by Shuk-kwan, Lee, 李淑君, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Characterization of Two-Pore Channel 2 by Nuclear Membrane Electrophysiology Submitted by Lee Shuk Kwan for the degree of Doctor of Philosophy at The University of Hong Kong in July 2016 2+ NAADP-mediated lysosomal calcium (Ca ) release induces signalling cascades that regulate various cellular processes. The discovery of two-pore channel 2 2+ (TPC2) as the NAADP receptor advances our understanding of lysosomal Ca signalling, yet the intracellular localization of lysosomes make them unamenable to traditional patch-clamp electrophysiology. Prior attempts to record TPC2 single-channel activity placed TPC2 outside its native environment and did not accurately reproduce the physiological properties of TPC2. To examine the feasibility of using nuclear membrane electrophysiology to characterize the TPC2 channel, we constructed a stable human TPC2-expressing DT40TKO cell line lacking endogenous InsP3Rs and RyRs (DT40TKO-hTPC2). Immunocytochemistry revealed hTPC2 expression on the ER and nuclear envelope. Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 2+ cells evoked cytosolic Ca transients, suggesting that the expressed hTPC2 was i functionally active. Utilizing nuclear membrane patch clamp electrophysiology, we detected a 220 pS single-channel current activated by NAADP with K as the permeant ion. The recorded single-channel currents displayed a linear current- voltage relationship, were sensitive to Ned-19 inhibition, were biphasically regulated by NAADP concentration, and were regulated by PKA phosphorylation. In conclusion, we developed a novel cell model for the characterization of the TPC2 channel, and nuclear membrane patch-clamp methodology provided an alternative approach to robustly investigate the electrophysiological properties of TPC2 with minimal manipulation. (Word count: 209) ii Subjects: Electrophysiology
Author: Douglas P. Zipes
Publisher: Elsevier Health Sciences
ISBN: 145572856X
Category : Medical
Languages : en
Pages : 1395
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Book Description
Cardiac Electrophysiology: From Cell to Bedside puts the latest knowledge in this subspecialty at your fingertips, giving you a well-rounded, expert grasp of every cardiac electrophysiology issue that affects your patient management. Drs. Zipes, Jalife, and a host of other world leaders in cardiac electrophysiology use a comprehensive, multidisciplinary approach to guide you through all of the most recent cardiac drugs, techniques, and technologies. Get well-rounded, expert views of every cardiac electrophysiology issue that affects your patient management from preeminent authorities in cardiology, physiology, pharmacology, pediatrics, biophysics, pathology, cardiothoracic surgery, and biomedical engineering from around the world. Visually grasp and easily absorb complex concepts through an attractive full-color design featuring color photos, tables, flow charts, ECGs, and more! Integrate the latest scientific understanding of arrhythmias with the newest clinical applications, to select the right treatment and management options for each patient. Stay current on the latest advancements and developments with sweeping updates and 52 NEW chapters - written by many new authors - on some of the hottest cardiology topics, such as new technologies for the study of the molecular structure of ion channels, molecular genetics, and the development of new imaging, mapping and ablation techniques. Get expert advice from Dr. Douglas P. Zipes - a leading authority in electrophysiology and editor of Braunwald's Heart Disease and the Heart Rhythm Journal - and Dr. Jose Jalife - a world-renowned leader and researcher in basic and translational cardiac electrophysiology. Access the full text online at Expert Consult, including supplemental text, figures, tables, and video clips. Your purchase entitles you to access the web site until the next edition is published, or until the current edition is no longer offered for sale by Elsevier, whichever occurs first. If the next edition is published less than one year after your purchase, you will be entitled to online access for one year from your date of purchase. Elsevier reserves the right to offer a suitable replacement product (such as a downloadable or CD-ROM-based electronic version) should online access to the web site be discontinued.
