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Author: C Hawes
Publisher: Academic Press
ISBN: 0323148980
Category : Science
Languages : en
Pages : 477
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Book Description
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Author: C Hawes
Publisher: Academic Press
ISBN: 0323148980
Category : Science
Languages : en
Pages : 477
Get Book Here
Book Description
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Author: John Lloyd Hall
Publisher: Elsevier-North-Holland Biomedical Press
ISBN:
Category : Science
Languages : en
Pages : 464
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Book Description
Author: William V. Dashek
Publisher: Springer Science & Business Media
ISBN: 1592592325
Category : Science
Languages : en
Pages : 297
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Book Description
Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 146840010X
Category : Science
Languages : en
Pages : 368
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Book Description
In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Author: Rudolf A. Steinbrecht
Publisher: Springer Science & Business Media
ISBN: 3642728154
Category : Science
Languages : en
Pages : 308
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Book Description
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Author:
Publisher: Academic Press
ISBN: 0123914388
Category : Science
Languages : en
Pages : 465
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Book Description
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. - Covers many areas of CLEM by the best international scientists in the field - Includes a brief history and overview on CLEM methods
Author: Deepesh De
Publisher: CSIRO PUBLISHING
ISBN: 0643099441
Category : Science
Languages : en
Pages : 314
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Book Description
This book is the only comprehensive work, at introductory level, on plant cell vacuoles. Vacuoles are ubiquitous, multifaceted and indispensable organelles and yet they have been thinly treated in the literature to date. This is at odds with the amount of interest in vacuoles that has been expressed in the last two decades. This comprehensive work provides a solid foundation on vacuoles to an advanced level. The latest research findings have been included in all aspects of plant and yeast vacuoles. The book synthesizes all the available information on the plant cell vacuole. It includes methodologies, occurrence and diversity, structure and biochemistry of tonoplasts and molecular biology of biogenesis and diverse functions, all presented in a concise way. The tremendous surge in the genetic engineering of plants for commercial products requires a comprehension of the functions and possibilities of vacuole manipulation since most of the targets of improvement directly involve vacuoles. Thus the work will be valuable to students of plant sciences, plant breeding, cell biology and plant biotechnology, as well as advanced researchers who seek a better understanding of this vital organelle.
Author: Trevor L. Wang
Publisher: CUP Archive
ISBN: 9780521314800
Category : Medical
Languages : en
Pages : 260
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Book Description
Immunology is rapidly generating new insights into all areas of the plant sciences. In this volume, various disciplines in the plant sciences are brought together under the unifying theme of Immunology. New applications of both antisera and monoclonal antibodies are presented in the context of recent research in the fields of plant physiology, plant development and molecular biology. Each chapter comprises a broad review written by an international scientist of the immunological aspects of current plant studies with a particular emphasis on techniques. The presentation of these step-by-step techniques appended to each chapter will make this volume of practical interest to both the advanced undergraduate and research worker in plant biology.
Author: S.M. Khasim
Publisher: CRC Press
ISBN: 1040106234
Category : Science
Languages : en
Pages : 495
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Book Description
This book deals with the basic concepts of Plant Science including botanical micro technique and microtomy, staining techniques, molecular techniques, plant tissue culture, electron microscopy, and cryopreservation and germplasm storage. It is the outcome of several decades of research and teaching in plant biology to undergraduate and postgraduate students of Plant Science, Horticulture, Microbiology, and Biotechnology. Print edition not for sale in Bangladesh, Bhutan, India, Nepal, Pakistan, and Sri Lanka.
Author: Hans-Ferdinand Linskens
Publisher: Springer Science & Business Media
ISBN: 3642825877
Category : Science
Languages : en
Pages : 419
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Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the inavailability of many new analytical methods concealed in specialized journals not normally accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete, that experiments are difficult to reproduce. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since today there is still a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and the descriptions as applied to plant material so complete in themselves that there was little need to consult other publications.
