DNA Recognition and Transcription Activation by Catabolite Gene Activator Protein (CAP) PDF Download
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Author: Xiaoping Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Book Description
Author: Xiaoping Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Book Description
Author: Angelo Hermanjie Gunasekera
Publisher:
ISBN:
Category :
Languages : en
Pages : 476
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Author: Yuhong Zhou
Publisher:
ISBN:
Category :
Languages : en
Pages : 292
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Author: Nataliya Popovych
Publisher:
ISBN:
Category : Allosteric regulation
Languages : en
Pages : 164
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Book Description
The E.coli catabolite activator protein (CAP) is a universal transcriptional activator that regulates more than half of the cell's transcription units by responding to fluctuations of the cellular concentration of cAMP. Cyclic 3'-5'-adenosine monophosphate (cAMP) binding to CAP elicits an allosteric transition that switches CAP from the "off" state, which binds DNA weakly and nonspecifically, to the "on" state that binds DNA strongly and specifically. In the cAMP-bound state CAP binds to DNA sites located at or close to promoter sites and enhances RNA polymerase binding to the promoter by a simple recruitment mechanism thereby activating transcription. The solution structure of CAP in the absence of cAMP (apo-CAP) was determined by nuclear magnetic resonance (NMR) spectroscopy. Multidimensional heteronuclear NMR experiments in combination with a series of specifically labeled samples were used to accomplish structure determination. The structure of the 50-kDa apo-CAP provides atomic details of the allosteric transition that leads to activation of CAP for DNA binding. cAMP elicits a coil-to-helix transition that stabilizes a suboptimal coiled coil, which is extensively unwound in apo-CAP. Coiled-coil winding causes the DNA-binding domain to undergo a large rigid-body translation and rotation that poise the recognition helices for specific DNA binding. The results highlight the unique structural mechanisms that underlie activation of this prototypic transcription factor and provide an example of how effector-mediated structural changes can control the activity of regulatory proteins. In addition, we have also characterized a number of CAP* mutants in order to further understand the mechanism underlying the activation of CAP. NMR spectroscopy in combination with isothermal titration calorimetry (ITC) was used to delineate the structural and dynamic features of distinct conformational states in cAMP binding to CAP. The intrinsically cooperative binding of cAMP to CAP was characterized using the isolated effector binding domain CAPN (residues 1-138) in three conformational states: in the absence of cAMP (apo-CAPN), in singly liganded state (intermediate, cAMP1-CAPN), and in the doubly liganded state (cAMP2-CAPN). We have shown that allosteric interactions can be mediated exclusively by transmitted changes in protein motions. Thus, the observed protein allostery is exclusively dynamically driven.
Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Molecular genetics
Languages : en
Pages : 466
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Author: K. Shimizu
Publisher: Elsevier
ISBN: 1908818204
Category : Science
Languages : en
Pages : 497
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The metabolic regulation of a cell system is of critical importance in systems biology, and a robust model of these mechanisms is essential in predicting the effects on the metabolism of both the culture environment and the knockout of specific genes. Bacterial cellular metabolic systems focuses on this highly topical subject in relation to culture environment and provides a detailed analysis from gene level to metabolic level regulation, as well as offering a discussion of the most recent modelling approaches. The book begins with an introduction to metabolic mechanisms and to the metabolic regulation of a cell, before moving on to discussing the action of global regulators in response to a specific culture environment. The second half of the book examines conventional flux balance analysis and its applications, 13C-metabolic flux analysis, and the effect of a specific gene knockout on the metabolism. - Comprehensive account of metabolic regulation via global regulators in response to changes in the culture environment - Basic formulation of 13C-metabolic flux analysis based on 13C-labelling experiments - Systems biology approach for the modelling and computer simulation of the main metabolic pathways of a cell system
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 488
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Book Description
Author: Loriano Ballarin
Publisher: Academic Press
ISBN: 0128032537
Category : Medical
Languages : en
Pages : 326
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Book Description
Lessons in Immunity: From Single-cell Organisms to Mammals stems from the activity of the Italian Association of Developmental and Comparative Immunobiology (IADCI), represented by the editors. This book is presented as a series of short overviews that report on the current state of various relevant fields of immunobiology from an evolutionary perspective. The overviews are written by authors directly involved in the research, and most are members of the IADCI or have otherwise been involved in the related research for their respective overview. This publication offers scientists and teachers an easy and updated reference tool. - Provides simple and updated reviews on the immunobiology of a wide spectrum of organisms, considered in an evolutionary context - Focuses on both cells and humoral components of a variety of non-classical model organisms - Offers in a single volume many contributions which can help with understanding the evolution of immune responses and the main adaptations in animal phyla - Presents a valuable holistic cross-sectional approach for teaching immunology and its applications
Author: Bryan M. Turner
Publisher: John Wiley & Sons
ISBN: 0865427437
Category : Science
Languages : en
Pages : 312
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Book Description
Written in an informal and accessible style, Chromatin and Gene Regulation enables the reader to understand the science of this rapidly moving field. Chromatin is a fundamental component in the network of controls that regulates gene expression. Many human diseases have been linked to disruption of these control processes by genetic or environmental factors, and unravelling the mechanisms by which they operate is one of the most exciting and rapidly developing areas of modern biology. Chromatin is central both to the rapid changes in gene transcription by which cells respond to changes in their environment and also to the maintenance of gene expression patterns from one cell generation to the next. This book will be an invaluable guide to undergraduate and postgraduate students in the biological sciences and all those with an interest in the medical implications of aberrant gene expression.
